PPP1R7 (NM_001282414) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	SDS22
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282414, the custom clone sequence may differ by one or more nucleotides ATGGAAGAACATGAGCTGCCTGTGGACATGGAAACCATCAACCTGGACAGAGATGCAGAGGATGTTGATT TGAATCACTATCGCATAGGGAAGATTGAAGGATTTGAGGTACTGAAGAAAGTGAAGACTCTCTGCCTCCG CCAAAATTTAATTAAATGCATTGAGAATCTGGAGGAGCTACAGAGTCTTCGAGAGCTGGATCTTTACGAC AACCAGATCAAGAAGATTGAGAATCTGGAGGCGCTAACAGAGCTGGAGATTCTAGATATTTCTTTTAATC TGCTGAGAAACATCGAAGGGGTTGACAAGTTGACACGACTGAAAAAACTCTTCTTGGTCAACAATAAAAT CAGTAAAATTGAGAACTTAAGCAACTTACATCAACTACAGATGCTAGAGCTGGGATCTAACCGCATCCGG GCAATCGAAAATATCGACACCTTAACCAACCTGGAGAGTTTGTTTTTGGGGAAAAACAAAATTACTAAAC TTCAGAACCTGGATGCGCTCACCAACCTGACAGTCCTCAGTATGCAGAGCAACCGGCTGACCAAGATCGA GGGTCTGCAGAACCTGGTGAACCTGCGGGAGCTGTACCTTAGCCACAATGGCATCGAGGTCATCGAGGGC CTGGAGAACAATGTGCAGGACAGCCTCACGTACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001282414
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282414.1, NP_001269343.1
RefSeq Size	920 bp
RefSeq ORF	666 bp
Locus ID	5510
UniProt ID	Q15435
Protein Families	Druggable Genome, Phosphatase
Gene Summary	This gene encodes a protein subunit that regulates the activity of the serine/threonine phosphatase, protein phosphatase-1. The encoded protein is required for completion of the mitotic cycle and for targeting protein phosphatase-1 to mitotic kinetochores. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (7) differs in the 5' and 3' UTRs and has multiple coding region differences compared to variant 1. The encoded protein (isoform 7) is shorter and has distinct N- and C-termini compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.