CKAP1 (TBCB) (NM_001300971) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CG22; CKAP1; CKAPI
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001300971, the custom clone sequence may differ by one or more nucleotides ATGGAACTGGAGCTGTATGGAGTTGACGACAAGTTCTACAGCAAGCTGGATCAAGAGGATGCGCTCCTGG GCTCCTACCCTGTAGATGACGGCTGCCGCATCCACGTCATTGACCACAGTGGCGCCCGCCTTGGTGAGTA TGAGGACGTGTCCCGGGTGGAGAAGTACACGATCTCACAAGAAGCCTACGACCAGAGGCAAGACACGGTC CGCTCTTTCCTGAAGCGCAGCAAGCTCGGCCGGTACAACGAGGAGGAGCGGGCTCAGCAGGAGGCCGAGG CCGCCCAGCGCCTGGCCGAGGAGAAGGCCCAGGCCAGCTCCATCCCCGTGGGCAGCCGCTGTGAGGTGCG GGCGGCGGGACAATCCCCTCGCCGGGGCACCGTCATGTATGTAGGTCTCACAGATTTCAAGCCTGGCTAC TGGATTGGTGTCCGCTATGATGAGCCACTGGGGAAAAATGATGGCAGTGTGAATGGGAAACGCTACTTCG AATGCCAGGCCAAGTATGGCGCCTTTGTCAAGCCAGCAGTCGTGACGGTGGGGGACTTCCCGGAGGAGGA CTACGGGTTGGACGAGATATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001300971
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001300971.1, NP_001287900.1
RefSeq Size	900 bp
RefSeq ORF	582 bp
Locus ID	1155
UniProt ID	Q99426
Gene Summary	Binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer (PubMed:9265649). Involved in regulation of tubulin heterodimer dissociation. May function as a negative regulator of axonal growth (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 2, which has a shorter N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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