GGT6 (NM_001288704) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC334107 representing NM_001288704. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGCGGGCAGAAGAGCCCGTGGTCTATCAGAAGCTGCTGCCCTGGGAGCCAAGCTTGGAGTCGGAG GAGGAAGTGGAGGAGGAGGAGACATCAGAGGCGCTGGTTCTAAACCCCCGGAGGCACCAGGACTCTTCC AGGAACAAGGCTGGCGGGCTGCCCGGAACCTGGGCCCGTGTAGTGGCAGCCCTGCTGCTGCTGGCTGTT GGCTGCTCCCTGGCTGTGAGGCAGCTCCAGAATCAGGGCAGGTCGACAGGAAGCTTGGGCTCTGTGGCC CCTCCACCCGGCGGACACTCCCACGGCCCTGGCGTATACCACCACGGTGCCATCATCAGCCCTGCAGAC TGCTGTGAGCCCCGAGAGCAGTGCCCTGGCCGCCGTGGACAGCAGCGGCTCTGTGCTCCTTCTCACCTC CTCGCTCAACTGCTCCTTTGGCTCTGCACACCTGTCCCCAAGCACTGGGGTTCTGCTCAGCAACCTGGT GGCCAAGTCTACCACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001288704
Insert Size	501 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001288704.1
RefSeq Size	1966 bp
RefSeq ORF	501 bp
Locus ID	124975
UniProt ID	Q6P531
Protein Pathways	Arachidonic acid metabolism, Cyanoamino acid metabolism, Glutathione metabolism, Metabolic pathways, Selenoamino acid metabolism, Taurine and hypotaurine metabolism
MW	18 kDa
Gene Summary	GGT6 belongs to the gamma-glutamyltransferase (GGT; EC 2.3.2.2) gene family. GGT is a membrane-bound extracellular enzyme that cleaves gamma-glutamyl peptide bonds in glutathione and other peptides and transfers the gamma-glutamyl moiety to acceptors. GGT is also key to glutathione homeostasis because it provides substrates for glutathione synthesis (Heisterkamp et al., 2008 [PubMed 18357469]).[supplied by OMIM, Oct 2008] Transcript Variant: This variant (5) lacks an alternate exon in the 5' coding region and uses an alternate splice site in the 3' coding region, which results in a frameshift and an early stop codon, compared to variant 3. It encodes isoform e, which is shorter and has a distinct C-terminus compared to isoform c.

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