VSTM1 (NM_001288791) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	SIRL-1; SIRL1; UNQ3033
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC333961 representing NM_001288791. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGAAAAAGAATGGGTCCCACTCGGTTGCCCAGGCTGGAGTGCAGTGGTGCAATCACAGCTCACTGC AGCCTTGACCTCCCAGGCCCAGATAAACACGATGAACTTGAAGCTCCCTCAATGAAAACAGACACCAGA ACCATCTTTGTCGCCATCTTCAGCTGCATCTCCATCCTTCTCCTCTTCCTCTCAGTCTTCATCATCTAC AGATGCAGCCAGCACAGTTCATCATCTGAGGAATCCACCAAGAGAACCAGCCATTCCAAACTTCCGGAG CAGGAGGCTGCCGAGGCAGATTTATCCAATATGGAAAGGGTATCTCTCTCGACGGCAGACCCCCAAGGA GTGACCTATGCTGAGCTAAGCACCAGCGCCCTGTCTGAGGCAGCTTCAGACACCACCCAGGAGCCCCCA GGATCTCATGAATATGCGGCACTGAAAGTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001288791
Insert Size	447 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001288791.1
RefSeq Size	825 bp
RefSeq ORF	447 bp
Locus ID	284415
UniProt ID	Q6UX27
Protein Families	Transmembrane
MW	16.1 kDa
Gene Summary	Isoform 2: Behaves as a cytokine, promoting IL17A secretion by CD4+ T-cells, and differentiation and activation of IL17 producing helper T-cells (TH17).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks one exon and contains an alternate exon in the 5' coding region, which results in a frameshift and use of an alternate start codon, compared to variant 1. The encoded isoform (2) has a shorter and distinct N-terminus, compared to isoform 1.

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