NFAT5 (NM_173215) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NF-AT5; NFATL1; NFATZ; OREBP; TONEBP
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333307 representing NM_173215. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGGCGGTGCTTGCAGCTCCTTTACCACCTCTTCCAGCCCTACCATTTATTCTACCTCAGTCACCGAC AGCAAGGCTATGCAAGTGGAGAGCTGCTCCTCAGCCGTGGGGGTAAGTAACAGAGGGGTAAGTGAAAAG CAGTTAACCAGTAACACAGTTCAGCAGCATCCATCAACACCGAAGAGGCACACAGTCTTGTACATCTCA CCACCACCTGAGGACTTGCTGGATAACAGTCGGATGTCCTGCCAGGATGAGGGGTGTGGATTGGAATCT GAGCAGAGCTGCAGTATGTGGATGGAGGATTCCCCCTCCAACTTCAGTAACATGAGCACCAGTTCCTAC AATGATAACACTGAGGTACCTCGTAAATCACGAAAACGAAATCCAAAGCAGAGGCCGGGGGTCAAACGA CGAGATTGTGAAGAATCTAATATGGATATATTTGATGCCGACAGTGCCAAAGCACCTCACTATGTGCTT TCTCAGCTTACCACGGACAACAAAGGCAACTCAAAAGCGGGAAATGGAACATTGGAAAACCAAAAAGGA ACTGGAGTAAAGAAGAGCCCTATGTTGTGTGGACAATATCCTGTTAAAAGTGAGGGAAAGGAGCTGAAG ATAGTTGTACAACCTGAGACACAGCACCGAGCTCGGTACCTGACTGAGGGCAGCCGTGGCTCAGTGAAA GATAGAACACAGCAAGGCTTTCCTACAGTAAAGCTGGAAGGCCATAATGAACCTGTAGTGTTGCAAGTG TTTGTGGGCAACGACTCTGGACGAGTGAAACCACATGGATTTTATCAGGCCTGCAGAGTAACTGGACGA AATACAACTCCTTGCAAAGAAGTGGACATTGAAGGCACTACTGTTATAGAAGTCGGCCTTGATCCTAGC AACAACATGACACTGGCGGTGGACTGCGTAGGGATATTGAAATTGAGGAATGCTGATGTCGAAGCCAGA ATAGGAATTGCTGGTTCCAAGAAGAAAAGCACTCGTGCCAGATTGGTTTTTCGAGTTAATATCATGAGG AAAGATGGCTCCACTTTGACACTGCAAACACCCTCTTCTCCAATTTTGTGTACTCAGCCAGCAGGAGTG CCAGAAATCTTAAAGAAAAGCTTGCATAGCTGTTCAGTGAAAGGAGAAGAAGAAGTGTTTTTAATCGGC AAGAACTTTCTGAAAGGAACTAAAGTTATTTTCCAAGAAAATGTTTCTGATGAAAACTCTTGGAAGTCA GAAGCTGAAATTGATATGGAACTATTTCATCAGAATCATCTTATTGTGAAGGTTCCTCCCTATCATGAC CAACATATAACTTTGCCTGTGTCAGTGGGAATATATGTAGTGACAAATGCTGGAAGATCTCATGATGTT CAACCATTCACTTACACTCCAGACCCAGCAGCAGCTGGTGCTTTGAATGTAAATGTGAAGAAGGAAATA TCTAGTCCAGCAAGACCTTGCTCTTTTGAAGAGGCCATGAAAGCAATGAAAACTACTGGATGTAATTTA GATAAGGTAAATATTATCCCTAATGCCCTGATGACTCCACTCATACCAAGCAGTATGATTAAGAGTGAA GATGTTACTCCAATGGAAGTAACAGCAGAAAAAAGATCTTCCACTATTTTTAAGACTACAAAGTCTGTT GGATCAACTCAGCAAACATTAGAAAACATCTCAAACATAGCAGGAAATGGCTCTTTTTCATCACCATCA TCTTCCCACCTACCTTCTGAAAATGAAAAACAGCAGCAGATTCAGCCCAAGGCATACAACCCAGAGACC CTGACAACTATTCAAACCCAGGACATCTCACAGCCTGGTACTTTTCCAGCAGTTTCTGCTTCTAGTCAG CTGCCCAACAGCGATGCACTATTGCAGCAGGCTACACAGTTTCAGACAAGAGAAACTCAGTCTAGAGAG ATATTACAGTCAGATGGTACAGTGGTTAATTTGTCACAACTGACTGAGGCATCACAACAACAGCAGCAG TCACCACTACAAGAACAAGCACAGACTTTACAGCAGCAGATTTCATCAAATATTTTTCCATCACCAAAT AGTGTGAGTCAGCTTCAGAATACTATTCAGCAGCTGCAAGCAGGGAGTTTCACAGGCAGTACTGCTAGT GGCAGCAGTGGAAGTGTTGACTTGGTCCAACAAGTTTTAGAGGCACAGCAGCAGTTATCTTCAGTTTTA TTTTCTGCTCCAGATGGTAATGAGAATGTTCAAGAGCAGCTTAGTGCAGATATTTTTCAACAAGTCAGT CAAATTCAGAGTGGTGTAAGCCCTGGAATGTTTTCCTCAACAGAGCCAACAGTCCATACCAGACCAGAT AATTTATTACCTGGAAGAGCTGAAAGTGTTCATCCACAGTCTGAAAACACGTTATCTAATCAACAGCAG CAGCAGCAGCAGCAACAGCAAGTGATGGAATCTTCAGCCGCAATGGTGATGGAGATGCAACAGAGTATC TGCCAGGCAGCTGCCCAGATTCAGTCAGAGTTATTCCCTTCAACTGCTTCAGCAAATGGAAACCTTCAG CAATCGCCAGTTTACCAGCAGACTTCTCACATGATGAGTGCATTGTCTACCAATGAGGATATGCAAATG CAGTGTGAATTGTTTTCTTCTCCTCCTGCAGTTTCTGGAAATGAAACTTCTACAACTACCACACAGCAG GTTGCAACCCCTGGCACTACCATGTTTCAGACATCAAGTTCAGGAGATGGAGAAGAAACTGGAACACAA GCAAAACAGATTCAGAACAGTGTCTTTCAGACCATGGTCCAAATGCAACATAGTGGGGACAATCAACCT CAAGTTAACCTTTTTTCATCCACAAAAAGTATGATGAGTGTTCAGAATAGTGGTACCCAACAACAAGGT AATGGTTTATTCCAGCAAGGGAATGAGATGATGTCACTTCAATCTGGAAATTTTTTGCAGCAGTCTTCT CATTCACAGGCCCAACTTTTTCATCCTCAAAATCCTATTGCCGATGCTCAGAACCTTTCCCAGGAAACT CAAGGTTCTCTCTTTCATAGTCCAAATCCTATTGTCCACAGTCAGACTTCTACAACCTCCTCTGAACAA ATGCAGCCTCCAATGTTTCACTCTCAAAGTACCATTGCTGTGTTACAGGGCTCTTCAGTTCCTCAAGAC CAGCAGTCAACCAACATATTTCTTTCCCAGAGTCCCATGAATAATCTTCAGACTAACACAGTAGCCCAA GAAGCATTTTTTGCAGCACCGAACTCAATTTCTCCACTTCAGTCAACATCAAACAGTGAACAACAAGCT GCTTTCCAACAGCAAGCTCCAATATCACACATCCAGACTCCTATGCTTTCCCAAGAACAGGCACAACCC CCGCAGCAGGGTTTATTTCAGCCTCAGGTGGCCCTGGGCTCCCTTCCACCTAATCCAATGCCTCAAAGC CAACAAGGAACCATGTTCCAGTCACAGCACTCAATAGTTGCCATGCAGAGTAACTCTCCATCCCAGGAA CAGCAGCAGCAGCAGCAACAGCAGCAGCAACAGCAGCAGCAACAACAACAGAGCATTTTATTCAGTAAT CAGAATACCATGGCTACAATGGCGTCTCCAAAGCAACCACCACCAAACATGATATTCAACCCAAATCAA AATCCAATGGCTAATCAGGAGCAACAGAACCAGTCAATTTTTCACCAACAAAGTAACATGGCCCCAATG AATCAAGAGCAACAGCCCATGCAATTTCAGAGTCAGTCCACAGTTTCCTCACTTCAGAACCCAGGTCCT ACCCAGTCGGAATCATCACAGACCCCCTTGTTCCATAGCTCTCCTCAGATTCAGTTGGTACAAGGGTCA CCTAGTTCTCAAGAGCAGCAAGTAACTCTCTTCTTATCTCCAGCATCCATGTCTGCCTTGCAGACCAGT ATAAATCAACAAGATATGCAACAGTCTCCTCTTTATTCCCCTCAGAACAACATGCCTGGAATTCAAGGA GCCACATCTTCGCCTCAACCACAGGCTACTTTATTTCACAACACAGCAGGAGGCACAATGAACCAACTG CAGAATTCTCCTGGCTCATCTCAGCAGACATCAGGAATGTTCTTATTTGGCATTCAAAATAACTGTAGT CAGCTTTTAACCTCTGGACCAGCTACATTGCCTGATCAGTTGATGGCCATAAGTCAGCCAGGCCAACCA CAAAACGAGGGCCAGCCACCTGTGACAACACTTCTTTCTCAGCAAATGCCAGAGAATTCTCCACTGGCA TCCTCTATAAACACCAACCAGAACATCGAAAAGATTGATTTGCTTGTTTCATTGCAAAACCAAGGGAAC AACTTGACTGGCTCCTTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_173215
Insert Size	4368 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_173215.2
RefSeq Size	5311 bp
RefSeq ORF	4368 bp
Locus ID	10725
UniProt ID	O94916
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Axon guidance, B cell receptor signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
MW	158 kDa
Gene Summary	The product of this gene is a member of the nuclear factors of activated T cells family of transcription factors. Proteins belonging to this family play a central role in inducible gene transcription during the immune response. This protein regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of this protein family, this protein exists as a homodimer and forms stable dimers with DNA elements. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (5) differs in the 5' and 3' UTRs, compared to variant 1. It encodes isoform a, which is also encoded by variants 1 and 4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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