TAOK1 (NM_025142) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	hKFC-B; hTAOK1; KFC-B; MAP3K16; MARKK; PSK-2; PSK2; TAO1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333295 representing NM_025142. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCATCAACTAACAGAGCAGGCAGCCTGAAGGACCCTGAAATTGCAGAGCTCTTCTTCAAAGAAGAT CCAGAGAAGCTCTTCACAGATCTCAGAGAAATTGGCCATGGAAGCTTTGGAGCAGTGTATTTTGCACGA GATGTGCGTACCAATGAAGTGGTGGCCATCAAGAAAATGTCTTATAGTGGAAAGCAGTCTACTGAGAAA TGGCAGGATATTATTAAGGAAGTCAAGTTTCTACAAAGAATAAAACATCCCAACAGTATAGAATACAAA GGCTGTTATTTACGTGAACACACAGCATGGCTTGTAATGGAATATTGTTTAGGATCTGCTTCGGATTTA CTAGAAGTTCACAAAAAGCCATTACAAGAAGTGGAAATAGCAGCAATTACACATGGTGCTCTTCAGGGA TTAGCCTACTTACATTCTCATACTATGATTCATAGAGATATCAAAGCAGGAAATATCCTTCTGACAGAA CCAGGCCAGGTGAAACTTGCTGACTTTGGCTCTGCTTCCATGGCATCACCTGCCAATTCCTTTGTGGGA ACGCCGTATTGGATGGCCCCAGAAGTAATTTTAGCCATGGATGAAGGACAATATGATGGCAAAGTAGAT GTGTGGTCTCTTGGAATAACATGTATTGAACTAGCGGAAAGGAAGCCTCCTTTATTTAATATGAATGCA ATGAGTGCCTTATATCACATAGCCCAAAATGAATCCCCTACACTACAGTCTAATGAATGGTCTGATTAT TTTCGCAACTTTGTAGATTCTTGCCTCCAGAAAATCCCTCAAGATCGACCTACATCAGAGGAACTTTTA AAGCACATATTTGTTCTTCGGGAGCGCCCTGAAACCGTGTTAATAGATCTCATTCAGAGGACAAAGGAT GCAGTAAGAGAGCTGGACAATCTGCAGTATCGAAAGATGAAGAAACTCCTTTTCCAGGAGGCACATAAT GGACCAGCAGTAGAAGCACAGGAAGAAGAAGAGGAACAAGATCATGGTGTTGGCCGGACAGGAACAGTT AATAGTGTTGGAAGTAATCAATCCATTCCCAGCATGTCCATCAGTGCCAGCAGCCAAAGCAGTAGTGTT AACAGTCTTCCAGATGTCTCAGATGACAAGAGTGAGCTAGACATGATGGAGGGAGACCACACAGTGATG TCTAACAGTTCTGTTATCCATTTAAAACCAGAGGAAGAAAATTACAGAGAAGAGGGAGATCCTAGAACA AGAGCATCAGATCCACAATCTCCACCCCAAGTATCTCGTCACAAATCACACTATCGTAATCGAGAACAC TTTGCTACTATACGGACAGCATCACTGGTTACGAGGCAAATGCAAGAACATGAGCAGGACTCTGAGCTT AGAGAACAAATGTCTGGCTATAAGCGAATGAGGCGACAACATCAAAAGCAACTGATGACTCTGGAAAAC AAGCTAAAGGCTGAGATGGATGAACATCGCCTCAGATTAGACAAAGATCTTGAAACTCAGCGTAACAAT TTTGCTGCAGAAATGGAGAAACTTATCAAGAAACACCAGGCTGCCATGGAGAAAGAGGCTAAAGTGATG TCCAATGAAGAGAAAAAATTTCAGCAACATATTCAGGCCCAACAGAAGAAAGAACTGAATAGTTTTCTC GAGTCCCAGAAAAGAGAGTATAAACTTCGAAAAGAGCAGCTTAAAGAGTCTAAAGAACTCCAAATAAAA AAGCAGTTTCAGGATACCTGCAAAATCCAAACCAGACAGTACAAAGCATTAAGAAATCACCTGCTGGAG ACTACACCAAAGAGTGAGCACAAAGCTGTTCTGAAACGGCTCAAGGAGGAACAGACCCGGAAATTAGCT ATCTTGGCTGAGCAGTATGATCACAGCATTAATGAAATGCTCTCCACACAAGCCCTGCGTTTGGATGAA GCACAGGAAGCAGAGTGCCAGGTTTTGAAGATGCAGCTGCAGCAGGAACTGGAGCTGTTGAATGCGTAT CAGAGCAAAATCAAGATGCAAGCTGAGGCACAACATGATCGAGAGCTTCGCGAGCTTGAACAGAGGGTC TCCCTCCGGAGGGCACTCTTAGAACAAAAGATTGAAGAAGAGATGTTGGCTTTGCAGAATGAGCGCACA GAACGAATACGAAGCCTGTTGGAACGTCAAGCCAGAGAGATTGAAGCTTTTGACTCTGAAAGCATGAGA CTAGGTTTTAGTAATATGGTCCTTTCTAATCTCTCCCCTGAGGCATTCAGCCACAGCTACCCGGGAGCT TCTGGTTGGTCACACAACCCTACTGGGGGTCCAGGACCTCACTGGGGTCATCCCATGGGTGGCCCACCA CAAGCTTGGGGCCATCCAATGCAAGGTGGACCCCAGCCATGGGGTCACCCTTCAGGGCCAATGCAAGGG GTACCTCGAGGTAGCAGTATGGGAGTCCGCAATAGCCCCCAGGCTCTGAGGCGGACAGCTTCTGGGGGA CGGACGGAGCAGGGCATGAGCAGAAGCACGAGTGTCACTTCACAAATATCCAATGGGTCACACATGTCT TATACATAA
Restriction Sites	SgfI-MluI
ACCN	NM_025142
Insert Size	2562 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_025142.1
RefSeq Size	11652 bp
RefSeq ORF	2562 bp
Locus ID	57551
UniProt ID	Q7L7X3
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	MAPK signaling pathway
MW	97.5 kDa
Gene Summary	Serine/threonine-protein kinase involved in various processes such as p38/MAPK14 stress-activated MAPK cascade, DNA damage response and regulation of cytoskeleton stability. Phosphorylates MAP2K3, MAP2K6 and MARK2. Acts as an activator of the p38/MAPK14 stress-activated MAPK cascade by mediating phosphorylation and subsequent activation of the upstream MAP2K3 and MAP2K6 kinases. Involved in G-protein coupled receptor signaling to p38/MAPK14. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of MAP2K3 and MAP2K6. Acts as a regulator of cytoskeleton stability by phosphorylating 'Thr-208' of MARK2, leading to activate MARK2 kinase activity and subsequent phosphorylation and detachment of MAPT/TAU from microtubules. Also acts as a regulator of apoptosis: regulates apoptotic morphological changes, including cell contraction, membrane blebbing and apoptotic bodies formation via activation of the MAPK8/JNK cascade.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame segment compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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