Bile salt activated lipase (CEL) (NM_001807) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BAL; BSDL; BSSL; CEase; CELL; FAP; FAPP; LIPA; MODY8
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333280 representing NM_001807. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCTCACCATGGGGCGCCTGCAACTGGTTGTGTTGGGCCTCACCTGCTGCTGGGCAGTGGCGAGTGCC GCGAAGCTGGGCGCCGTGTACACAGAAGGTGGGTTCGTGGAAGGCGTCAATAAGAAGCTCGGCCTCCTG GGTGACTCTGTGGACATCTTCAAGGGCATCCCCTTCGCAGCTCCCACCAAGGCCCTGGAAAATCCTCAG CCACATCCTGGCTGGCAAGGGACCCTGAAGGCCAAGAACTTCAAGAAGAGATGCCTGCAGGCCACCATC ACCCAGGACAGCACCTACGGGGATGAAGACTGCCTGTACCTCAACATTTGGGTGCCCCAGGGCAGGAAG CAAGTCTCCCGGGACCTGCCCGTTATGATCTGGATCTATGGAGGCGCCTTCCTCATGGGGTCCGGCCAT GGGGCCAACTTCCTCAACAACTACCTGTATGACGGCGAGGAGATCGCCACACGCGGAAACGTCATCGTG GTCACCTTCAACTACCGTGTCGGCCCCCTTGGGTTCCTCAGCACTGGGGACGCCAATCTGCCAGGTAAC TATGGCCTTCGGGATCAGCACATGGCCATTGCTTGGGTGAAGAGGAATATCGCGGCCTTCGGGGGGGAC CCCAACAACATCACGCTCTTCGGGGAGTCTGCTGGAGGTGCCAGCGTCTCTCTGCAGACCCTCTCCCCC TACAACAAGGGCCTCATCCGGCGAGCCATCAGCCAGAGCGGCGTGGCCCTGAGTCCCTGGGTCATCCAG AAAAACCCACTCTTCTGGGCCAAAAAGGTGGCTGAGAAGGTGGGTTGCCCTGTGGGTGATGCCGCCAGG ATGGCCCAGTGTCTGAAGGTTACTGATCCCCGAGCCCTGACGCTGGCCTATAAGGTGCCGCTGGCAGGC CTGGAGTACCCCATGCTGCACTATGTGGGCTTCGTCCCTGTCATTGATGGAGACTTCATCCCCGCTGAC CCGATCAACCTGTACGCCAACGCCGCCGACATCGACTATATAGCAGGCACCAACAACATGGACGGCCAC ATCTTCGCCAGCATCGACATGCCTGCCATCAACAAGGGCAACAAGAAAGTCACGGAGGAGGACTTCTAC AAGCTGGTCAGTGAGTTCACAATCACCAAGGGGCTCAGAGGCGCCAAGACGACCTTTGATGTCTACACC GAGTCCTGGGCCCAGGACCCATCCCAGGAGAATAAGAAGAAGACTGTGGTGGACTTTGAGACCGATGTC CTCTTCCTGGTGCCCACCGAGATTGCCCTAGCCCAGCACAGAGCCAATGCCAAGAGTGCCAAGACCTAC GCCTACCTGTTTTCCCATCCCTCTCGGATGCCCGTCTACCCCAAATGGGTGGGGGCCGACCATGCAGAT GACATTCAGTACGTTTTCGGGAAGCCCTTCGCCACCCCCACGGGCTACCGGCCCCAAGACAGGACAGTC TCTAAGGCCATGATCGCCTACTGGACCAACTTTGCCAAAACAGGGGACCCCAACATGGGCGACTCGGCT GTGCCCACACACTGGGAACCCTACACTACGGAAAACAGCGGCTACCTGGAGATCACCAAGAAGATGGGC AGCAGCTCCATGAAGCGGAGCCTGAGAACCAACTTCCTGCGCTACTGGACCCTCACCTATCTGGCGCTG CCCACAGTGACCGACCAGGAGGCCACCCCTGTGCCCCCCACAGGGGACTCCGAGGCCACTCCCGTGCCC CCCACGGGTGACTCCGAGACCGCCCCCGTGCCGCCCACGGGTGACTCCGGGGCCCCCCCCGTGCCGCCC ACGGGTGACTCCGGGGCCCCCCCCGTGCCGCCCACGGGTGACTCCGGGGCCCCCCCCGTGCCGCCCACG GGTGACTCCGGGGCCCCCCCCGTGCCGCCCACGGGTGACTCCGGGGCCCCCCCCGTGCCGCCCACGGGT GACTCCGGGGCCCCCCCCGTGCCGCCCACGGGTGACTCCGGGGCCCCCCCCGTGCCGCCCACGGGTGAC TCCGGCGCCCCCCCCGTGCCGCCCACGGGTGACGCCGGGCCCCCCCCCGTGCCGCCCACGGGTGACTCC GGCGCCCCCCCCGTGCCGCCCACGGGTGACTCCGGGGCCCCCCCCGTGACCCCCACGGGTGACTCCGAG ACCGCCCCCGTGCCGCCCACGGGTGACTCCGGGGCCCCCCCTGTGCCCCCCACGGGTGACTCTGAGGCT GCCCCTGTGCCCCCCACAGATGACTCCAAGGAAGCTCAGATGCCTGCAGTCATTAGGTTTTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001807
Insert Size	2271 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001807.4
RefSeq Size	2386 bp
RefSeq ORF	2271 bp
Locus ID	1056
Protein Families	Druggable Genome
Protein Pathways	Glycerolipid metabolism, Metabolic pathways, Steroid biosynthesis
MW	79.7 kDa
Gene Summary	The protein encoded by this gene is a glycoprotein secreted from the pancreas into the digestive tract and from the lactating mammary gland into human milk. The physiological role of this protein is in cholesterol and lipid-soluble vitamin ester hydrolysis and absorption. This encoded protein promotes large chylomicron production in the intestine. Also its presence in plasma suggests its interactions with cholesterol and oxidized lipoproteins to modulate the progression of atherosclerosis. In pancreatic tumoral cells, this encoded protein is thought to be sequestrated within the Golgi compartment and is probably not secreted. This gene contains a variable number of tandem repeat (VNTR) polymorphism in the coding region that may influence the function of the encoded protein. [provided by RefSeq, Jul 2008]

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