VWA2 (NM_001272046) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AMACO; CCSP-2; CCSP2; NET42
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333192 representing NM_001272046. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCCCCTTTCCTGTTGCTGGAAGCCGTCTGTGTTTTCCTGTTTTCCAGAGTGCCCCCATCTCTCCCT CTCCAGGAAGTCCATGTAAGCAAAGAAACCATCGGGAAGATTTCAGCTGCCAGCAAAATGATGTGGTGC TCGGCTGCAGTGGACATCATGTTTCTGTTAGATGGGTCTAACAGCGTCGGGAAAGGGAGCTTTGAAAGG TCCAAGCACTTTGCCATCACAGTCTGTGACGGTCTGGACATCAGCCCCGAGAGGGTCAGAGTGGGAGCA TTCCAGTTCAGTTCCACTCCTCATCTGGAATTCCCCTTGGATTCATTTTCAACCCAACAGGAAGTGAAG GCAAGAATCAAGAGGATGGTTTTCAAAGGAGGGCGCACGGAGACGGAACTTGCTCTGAAATACCTTCTG CACAGAGGGTTGCCTGGAGGCAGAAATGCTTCTGTGCCCCAGATCCTCATCATCGTCACTGATGGGAAG TCCCAGGGGGATGTGGCACTGCCATCCAAGCAGCTGAAGGAAAGGGGTGTCACTGTGTTTGCTGTGGGG GTCAGGTTTCCCAGGTGGGAGGAGCTGCATGCACTGGCCAGCGAGCCTAGAGGGCAGCACGTGCTGTTG GCTGAGCAGGTGGAGGATGCCACCAACGGCCTCTTCAGCACCCTCAGCAGCTCGGCCATCTGCTCCAGC GCCACGCCAGACTGCAGGGTCGAGGCTCACCCCTGTGAGCACAGGACGCTGGAGATGGTCCGGGAGTTC GCTGGCAATGCCCCATGCTGGAGAGGATCGCGGCGGACCCTTGCGGTGCTGGCTGCACACTGTCCCTTC TACAGCTGGAAGAGAGTGTTCCTAACCCACCCTGCCACCTGCTACAGGACCACCTGCCCAGGCCCCTGT GACTCGCAGCCCTGCCAGAATGGAGGCACATGTGTTCCAGAAGGACTGGACGGCTACCAGTGCCTCTGC CCGCTGGCCTTTGGAGGGGAGGCTAACTGTGCCCTGAAGCTGAGCCTGGAATGCAGGGTCGACCTCCTC TTCCTGCTGGACAGCTCTGCGGGCACCACTCTGGACGGCTTCCTGCGGGCCAAAGTCTTCGTGAAGCGG TTTGTGCGGGCCGTGCTGAGCGAGGACTCTCGGGCCCGAGTGGGTGTGGCCACATACAGCAGGGAGCTG CTGGTGGCGGTGCCTGTGGGGGAGTACCAGGATGTGCCTGACCTGGTCTGGAGCCTCGATGGCATTCCC TTCCGTGGTGGCCCCACCCTGACGGGCAGTGCCTTGCGGCAGGCGGCAGAGCGTGGCTTCGGGAGCGCC ACCAGGACAGGCCAGGACCGGCCACGTAGAGTGGTGGTTTTGCTCACTGAGTCACACTCCGAGGATGAG GTTGCGGGCCCAGCGCGTCACGCAAGGGCGCGAGAGCTGCTCCTGCTGGGTGTAGGCAGTGAGGCCGTG CGGGCAGAGCTGGAGGAGATCACAGGCAGCCCAAAGCATGTGATGGTCTACTCGGATCCTCAGGATCTG TTCAACCAAATCCCTGAGCTGCAGGGGAAGCTGTGCAGCCGGCAGCGGCCAGGGTGCCGGACACAAGCC CTGGACCTCGTCTTCATGTTGGACACCTCTGCCTCAGTAGGGCCCGAGAATTTTGCTCAGATGCAGAGC TTTGTGAGAAGCTGTGCCCTCCAGTTTGAGGTGAACCCTGACGTGACACAGGTCGGCCTGGTGGTGTAT GGCAGCCAGGTGCAGACTGCCTTCGGGCTGGACACCAAACCCACCCGGGCTGCGATGCTGCGGGCCATT AGCCAGGCCCCCTACCTAGGTGGGGTGGGCTCAGCCGGCACCGCCCTGCTGCACATCTATGACAAAGTG ATGACCGTCCAGAGGGGTGCCCGGCCTGGTGTCCCCAAAGCTGTGGTGGTGCTCACAGGCGGGAGAGGC GCAGAGGATGCAGCCGTTCCTGCCCAGAAGCTGAGGAACAATGGCATCTCTGTCTTGGTCGTGGGCGTG GGGCCTGTCCTAAGTGAGGGTCTGCGGAGGCTTGCAGGTCCCCGGGATTCCCTGATCCACGTGGCAGCT TACGCCGACCTGCGGTACCACCAGGACGTGCTCATTGAGTGGCTGTGTGGAGAAGCCAAGCAGCCAGTC AACCTCTGCAAACCCAGCCCGTGCATGAATGAGGGCAGCTGCGTCCTGCAGAATGGGAGCTACCGCTGC AAGTGTCGGGATGGCTGGGAGGGCCCCCACTGCGAGAACCGATTCTTGAGACGCCCCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001272046
Insert Size	2268 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001272046.1
RefSeq Size	5857 bp
RefSeq ORF	2268 bp
Locus ID	340706
UniProt ID	Q5GFL6
MW	82 kDa
Gene Summary	This gene encodes a member of the von Willebrand factor A-like domain protein superfamily. The encoded protein is localized to the extracellular matrix and may serve as a structural component in basement membranes or in anchoring structures on scaffolds of collagen VII or fibrillin. This gene has been linked to type 1A diabetes and is a candidate serological marker for colon cancer. [provided by RefSeq, Jan 2013] Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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