KCNT1 (NM_001272003) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bA100C15.2; DEE14; EIEE14; ENFL5; KCa4.1; SLACK; Slo2.2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333183 representing NM_001272003. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCACTCCCTGACGGGGCGCGGACCCCGGGGGGCGTCTGCCGGGAGGCGCGCGGCGGGGGCTACACC AACCGGACCTTCGAGTTTGACGACGGCCAATGCGCCCCCAGGGTCCAGGTGGAGTTCTACGTCAACGAG AACACCTTCAAGGAGCGGCTCAAGCTGTTCTTCATCAAAAACCAAAGATCGAGCCTGAGGATCCGGCTG TTCAACTTCTCCCTGAAGCTGCTCACCTGCCTGCTCTACATTGTGCGCGTCCTGCTCGATGACCCGGCC CTGGGCATCGGATGCTGGGGCTGCCCAAAGCAGAACTACTCCTTCAATGACTCGTCCTCCGAGATCAAC TGGGCTCCTATTCTGTGGGTGGAGAGAAAGATGACACTGTGGGCGATCCAGGTCATCGTGGCCATAATA AGCTTCCTGGAGACGATGCTTCTCATCTACCTCAGCTACAAAGGCAACATCTGGGAGCAGATCTTCCGC GTGTCCTTCGTCCTGGAGATGATCAACACTCTGCCCTTCATCATCACGATCTTCTGGCCGCCGCTGCGG AACCTGTTCATCCCCGTCTTTCTGAACTGCTGGCTGGCCAAGCACGCGCTGGAAAACATGATTAATGAC TTCCACCGTGCCATCCTGCGGACACAGTCAGCCATGTTCAACCAGGTCCTCATCCTCTTCTGCACCCTG CTGTGCCTCGTTTTCACGGGGGGTTGCAGGACCTGCGGCATCCAGCACCTGGAGCGGGCGGGCGAGAAC CTGTCCCTCCTGACCTCCTTCTACTTCTGCATCGTCACCTTCTCCACCGTGGGCTACGGTGACGTCACG CCCAAGATCTGGCCATCGCAGCTGCTGGTGGTCATCATGATCTGCGTGGCCCTCGTGGTGCTCCCACTG CAGTTCGAGGAGCTCGTCTACCTCTGGATGGAGCGGCAGAAGTCAGGGGGCAACTACAGCCGCCACCGT GCGCAGACGGAGAAGCACGTGGTCCTGTGTGTCAGCTCCCTCAAGATCGACCTTCTCATGGACTTCCTG AACGAGTTCTACGCCCACCCCCGGCTCCAGGACTATTACGTGGTCATCCTGTGCCCCACGGAGATGGAT GTCCAGGTGCGCAGAGTCCTGCAGATCCCTCTGTGGTCCCAGCGGGTCATCTACCTCCAGGGCTCTGCA CTCAAAGACCAGGACCTCATGCGAGCCAAGATGGACAATGGGGAGGCCTGCTTCATCCTCAGCAGCAGG AACGAGGTGGACCGCACGGCTGCAGACCACCAGACCATCCTGCGCGCCTGGGCCGTGAAGGACTTCGCC CCCAACTGCCCCCTCTACGTCCAGATCCTCAAACCTGAAAACAAGTTTCACGTCAAGTTTGCTGACCAC GTGGTGTGTGAGGAGGAGTGCAAGTACGCCATGCTGGCGCTGAACTGCATCTGCCCGGCGACCTCCACC CTCATCACCCTGCTGGTGCACACGTCCCGCGGCCAGGAGGGACAGGAGTCTCCGGAGCAGTGGCAGCGC ATGTATGGGCGCTGCTCCGGCAACGAGGTGTACCACATCCGCATGGGTGACAGCAAGTTCTTCCGCGAG TACGAGGGCAAGAGCTTCACCTACGCGGCCTTCCACGCCCACAAGAAGTATGGCGTGTGCCTCATCGGG CTGAAGCGGGAGGACAACAAGAGCATCCTGCTGAACCCGGGGCCCCGGCACATCCTGGCCGCCTCTGAC ACCTGCTTCTACATCAACATCACCAAGGAGGAGAACTCGGCCTTCATCTTCAAGCAGGAGGAGAAGCGG AAGAAGAGGGCCTTCTCGGGGCAGGGGCTGCACGAGGGTCCGGCCCGCCTGCCCGTGCACAGCATCATC GCCTCCATGGGGACAGTGGCCATGGACCTGCAGGGCACAGAGCACCGGCCTACGCAGAGCGGCGGTGGG GGCGGGGGCAGCAAGCTGGCACTGCCCACGGAGAACGGCTCGGGCAGCCGGCGGCCCAGCATCGCGCCC GTCCTGGAACTGGCCGACAGCTCAGCCCTGCTGCCCTGCGACCTGCTGAGCGACCAGTCGGAGGATGAG GTGACGCCGTCGGACGACGAGGGGCTCTCCGTGGTAGAGTATGTGAAGGGCTACCCTCCCAACTCGCCC TACATCGGCAGCTCCCCAACCCTGTGCCACCTCCTGCCTGTGAAAGCCCCCTTCTGCTGCCTGCGGCTG GACAAGGGCTGCAAGCACAACAGCTATGAAGACGCCAAGGCCTACGGGTTCAAGAACAAGCTGATCATC GTCTCGGCAGAGACGGCCGGCAATGGGCTGTACAACTTCATCGTGCCACTGCGGGCCTACTACAGATCC CGCAAGGAGCTGAACCCCATCGTGCTGCTGCTGGACAACAAGCCCGACCACCACTTCCTGGAAGCCATC TGCTGCTTCCCCATGGTCTACTACATGGAGGGCTCTGTGGACAACCTGGACAGCCTGCTGCAGTGTGGC ATCATCTATGCGGACAACCTGGTGGTGGTGGACAAGGAGAGCACCATGAGCGCCGAGGAGGACTACATG GCGGACGCCAAGACCATCGTCAACGTGCAGACCATGTTCCGGCTCTTCCCCAGCCTCAGCATCACCACG GAGCTCACCCACCCTTCCAACATGCGCTTCATGCAGTTCCGCGCCAAGGACAGCTACTCTCTGGCTCTT TCCAAACTAGAAAAGAGGGAGCGAGAGAATGGCTCCAACCTGGCCTTCATGTTCCGCCTGCCGTTCGCC GCCGGCCGCGTCTTCAGCATCAGCATGTTGGACACACTGCTCTACCAGTCCTTCGTGAAGGACTACATG ATCACCATCACCCGGCTGCTGCTGGGCCTGGACACCACGCCGGGCTCGGGGTACCTCTGTGCCATGAAA ATCACCGAGGGCGACCTGTGGATCCGCACGTACGGCCGCCTCTTCCAGAAGCTCTGCTCCTCCAGCGCC GAGATCCCCATTGGCATCTACCGGACAGAGAGCCACGTCTTCTCCACCTCGGAGCCCCACGACCTCAGA GCCCAGTCCCAGATCTCGGTGAACGTGGAGGACTGTGAGGACACACGGGAAGTGAAGGGGCCCTGGGGC TCCCGCGCTGGCACCGGAGGCAGCTCCCAGGGCCGCCACACGGGCGGCGGTGACCCCGCAGAGCACCCA CTGCTACGGCGCAAGAGCCTGCAGTGGGCCCGGAGGCTGAGCCGCAAGGCGCCCAAGCAGGCAGGCCGG GCGGCGGCCGCGGAGTGGATCAGCCAGCAGCGCCTCAGCCTGTACCGGCGCTCTGAGCGCCAGGAGCTC TCCGAGCTGGTGAAGAACCGCATGAAGCACCTGGGGCTGCCCACCACCGGCTACGAGGACGTAGCAAAT TTAACAGCCAGTGATGTCATGAATCGGGTAAACCTGGGATATTTGCAAGACGAGATGAACGACCACCAG AACACCCTCTCCTACGTCCTCATCAACCCTCCGCCCGACACGAGGCTGGAGCCCAGTGACATTGTCTAT CTCATCCGCTCCGACCCCCTGGCTCACGTGGCCAGCAGCTCCCAGAGCCGGAAGAGCAGCTGCAGCCAC AAGCTGTCGTCCTGCAACCCCGAGACTCGCGACGAGACACAGCTCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001272003
Insert Size	3636 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001272003.1
RefSeq Size	4696 bp
RefSeq ORF	3636 bp
Locus ID	57582
UniProt ID	Q5JUK3
Protein Families	Druggable Genome, Ion Channels: Potassium, Transmembrane
MW	137 kDa
Gene Summary	Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a sodium-activated potassium channel subunit which is thought to function in ion conductance and developmental signaling pathways. Mutations in this gene cause the early-onset epileptic disorders, malignant migrating partial seizures of infancy and autosomal dominant nocturnal frontal lobe epilepsy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012] Transcript Variant: This variant (2) lacks an alternate exon in the 5' coding region, uses an alternate splice site in the 5' region and contains an additional exon in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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