KIF27 (NM_001271927) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333154 representing NM_001271927. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAAGAAATACCAGTAAAAGTTGCTGTAAGAATTAGACCTCTGCTTTGCAAAGAAGCTCTTCATAAT CATCAAGTTTGTGTGAGAGTTATTCCAAACAGCCAGCAAGTTATCATTGGGAGAGATAGAGTCTTCACT TTTGATTTTGTTTTTGGCAAAAATTCCACTCAAGATGAAGTTTATAACACATGTATAAAGCCCCTAGTG TTGTCACTCATTGAGGGCTATAATGCAACTGTTTTTGCCTATGGACAAACTGGATCTGGGAAGACATAC ACCATTGGAGGGGGCCATATTGCTTCAGTTGTGGAGGGCCAAAAGGGTATCATTCCTCGAGCTATTCAA GAAATATTTCAAAGCATCTCTGAACATCCTAGCATTGACTTTAATGTAAAAGTATCTTATATAGAAGTG TACAAGGAAGACCTAAGAGATCTTCTAGAATTGGAGACATCCATGAAGGATCTTCACATCCGAGAAGAT GAAAAAGGAAACACAGTGATTGTTGGGGCCAAGGAATGCCATGTGGAGAGTGCAGGTGAAGTGATGAGT CTTTTGGAGATGGGGAATGCAGCCAGACATACAGGTACCACTCAAATGAATGAGCACTCCAGCAGATCA CATGCAATTTTTACAATCAGCATTTGTCAAGTTCATAAAAATATGGAGGCAGCTGAAGATGGATCATGG TATTCCCCTCGGCATATTGTCTCAAAGTTCCACTTTGTGGATTTGGCAGGATCAGAAAGAGTAACCAAA ACGGGGAATACTGGTGAACGGTTCAAAGAATCCATTCAAATCAATAGTGGATTGCTGGCTTTAGGAAAT GTAATAAGCGCTCTTGGGGACCCACGCAGGAAGAGTTCACATATTCCATATAGGGATGCTAAAATTACC CGGCTTCTGAAAGATTCTCTGGGAGGCAGTGCTAAGACTGTCATGATCACATGTGTCAGCCCCTCCTCC TCGAATTTTGATGAGTCCTTAAATTCTCTCAAATATGCCAACAGAGCACGGAACATTAGAAACAAACCC ACTGTAAACTTCAGCCCCGAGTCAGACCGTATAGATGAAATGGAATTTGAGATTAAATTGCTTCGAGAA GCTTTGCAAAGCCAGCAGGCTGGTGTCAGCCAAACTACCCAGATCAATCGAGAAGGGAGTCCTGATACA AATAGGATTCATTCTCTTGAGGAGCAAGTAGCTCAGCTTCAAGGAGAATGTCTGGGTTACCAGTGTTGT GTAGAAGAAGCCTTTACCTTCCTGGTTGACCTAAAAGATACTGTCAGACTAAACGAAAAGCAGCAACAC AAACTGCAGGAGTGGTTTAACATGATCCAAGAGGTCAGGAAGGCTGTCCTCACCTCATTTCGAGGAATC GGAGGCACTGCAAGTCTGGAAGAAGGACCACAGCATGTTACAGTTCTCCAGCTGAAGAGAGAGCTTAAG AAATGCCAGTGTGTGCTTGCTGCTGATGAAGTAGTATTTAATCAGAAGGAACTGGAGGTGAAGGAACTG AAGAATCAAGTGCAGATGATGGTACAGGAAAACAAAGGGCATGCTGTATCTTTGAAAGAAGCGCAAAAA GTGAATAGACTGCAGAATGAAAAAATAATAGAACAACAACTTCTTGTGGATCAACTGAGTGAAGAACTA ACAAAACTTAACCTGTCAGTGACTTCTTCAGCTAAAGAAAATTGTGGAGATGGGCCAGATGCCAGGATC CCTGAAAGGAGACCATATACTGTACCATTTGATACTCATTTGGGGCATTATATTTATATCCCATCAAGA CAAGATTCCAGGAAGGTCCACACAAGTCCGCCTATGTACTCTCTGGATCGAATATTTGCTGGATTTCGA ACACGAAGTCAGATGCTGTTGGGTCACATAGAAGAACAAGATAAGGTCCTCCACTGCCAATTTTCTGAT AACAGTGATGATGAAGAATCAGAAGGCCAAGAGAAATCTGGAACTAGATGTAGAAGTCGTTCATGGATT CAGAAGCCAGACTCTGTTTGTTCCCTTGTTGAATTGAGTGATACTCAGGATGAAACACAAAAGTCAGAT TTGGAGAATGAAGATTTAAAGATTGATTGTCTCCAGGAGAGTCAAGAATTGAATTTGCAAAAATTAAAG AATTCAGAACGCATACTTACTGAAGCTAAACAAAAAATGAGAGAACTTACAATTAACATCAAGATGAAG GAAGATCTGATTAAAGAATTAATAAAAACAGGTAATGATGCCAAGTCTGTAAGCAAGCAGTATTCTTTG AAAGTAACAAAGCTAGAGCATGATGCAGAACAGGCAAAAGTCGAACTGATTGAAACACAAAAGCAGCTA CAGGAGCTGGAAAACAAAGATCTTTCTGATGTTGCAATGAAGGTAAAATTACAGAAAGAGTTTCGTAAA AAGATGGATGCTGCAAAGCTGAGAGTTCAGGAAATACAATTAAAAACAGGACAGGAAGAAGGTCTAAAA CCGAAAGCTGAGGACCTTGATGCATGTAACTTGAAAAGGAGAAAAGGTTCGTTTGGAAGTATAGACCAT CTCCAGAAATTGGATGAGCAAAAGAAATGGTTAGATGAAGAAGTAGAGAAAGTTCTGAACCAACGCCAA GAATTAGAGGAGCTGGAAGCAGACTTAAAGAAACGGGAGGCCATAGTTTCTAAGAAGGAGGCTCTGTTA CAGGAGAAGAGTCACCTGGAAAATAAGAAATTGAGATCTAGTCAGGCCTTAAACACAGATAGTTTGAAA ATATCAACTCGCCTGAACTTACTGGAACAAGAGTTGTCTGAAAAGAATGTGCAGCTCCAGACCAGTACA GCTGAGGAGAAAACAAAGATTTCAGAACAAGTTGAAGTCCTCCAGAAAGAAAAGGATCAGCTCCAGAAA CGCAGACACAATGTGGATGAAAAACTTAAAAATGGTAGAGTGTTATCACCTGAAGAAGAACATGTTCTT TTCCAACTTGAAGAAGGGATTGAAGCTTTGGAAGCTGCAATTGAATACAGGAATGAAAGTATCCAGAAT CGCCAGAAGTCACTTAGAGCATCATTCCATAACCTCTCTCGTGGTGAAGCAAATGTCTTGGAAAAGCTA GCTTGCCTGAGTCCTGTTGAGATTAGAACTATTCTTTTCAGATATTTCAATAAGGTGGTGAATTTGCGA GAAGCTGAACGGAAACAACAGTTATATAATGAAGAAATGAAAATGAAAGTTCTGGAACGGGATAATATG GTTCGTGAATTAGAATCTGCACTGGACCATCTAAAATTGCAGTGTGACCGGAGACTGACCCTCCAGCAA AAGGAACACGAACAAAAGATGCAGTTGCTATTACATCATTTCAAAGAACAAGATGGAGAAGGCATTATG GAAACTTTCAAAACATATGAAGATAAAATCCAGCAGTTGGAAAAAGATCTTTATTTCTATAAGAAAACC AGCCGGGATCATAAGAAGAAACTTAAGGAACTGGTAGGGGAAGCAATTCGGCGGCAACTAGCACCATCA GAGTATCAAGAGGCTGGAGATGGAGTCCTGAAGCCAGAAGGAGGAGGCATGCTTTCAGAAGAATTAAAA TGGGCATCCAGACCTGAAAGTATGAAATTAAGTGGAAGAGAAAGAGAAATGGACAGTTCAGCAAGCAGC TTAAGAACACAGCCAAATCCTCAAAAGCTCTGGGAAGATATCCCAGAATTACCTCCAATTCATAGTTCT TTAGCACCCCCCAGTGGGCATATGTTAGGTAATGAGAATAAAACAGAAACAGATGATAATCAGTTTACA AAATCTCACAGTCGACTGTCATCCCAAATTCAGGTTGTGGGAAATGTGGGACGACTTCATGGTGTCACA CCTGTAAAACTGTGTCGAAAAGAATTACGTCAAATTTCCGCCTTGGAACTATCATTGCGACGTTCCAGT CTTGGAGTTGGCATTGGATCAATGGCTGCTGATTCCATCGAAGTATCTAGGAAACCAAGGGACTTAAAA ACTTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001271927
Insert Size	4008 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271927.1
RefSeq Size	4493 bp
RefSeq ORF	4008 bp
Locus ID	55582
UniProt ID	Q86VH2
Protein Families	Druggable Genome
MW	152.3 kDa
Gene Summary	This gene is a member of the KIF27 (kinesin 4) sub-family of the mammalian kinesin family. The gene is an ortholog of the Drosophila Cos2 gene, which plays an important role in the Hedgehog signaling pathway. The encoded protein contains an N-terminal motor domain which includes nucleotide-binding and microtubule-interacting regions, a stalk domain containing a predicted coiled coil motif and a C-terminal tail domain. Alternatively spliced transcript variants have been observed for this gene. Pseudogenes associated with this gene are located on chromosome 9. [provided by RefSeq, Dec 2012] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1. The encoded isoform (B) is shorter than isoform A.

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