TINP1 (NSA2) (NM_001271665) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CDK105; HCL-G1; HCLG1; HUSSY-29; HUSSY29; TINP1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333079 representing NM_001271665. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCACAGAATGAATATATTGAATTACACCGTAAACGCTATGGATACCGTTTGGATTACCATGAGAAA AAGAGAAAGAAGGAAAGTCGAGAGGCTCATGAACGTTCAAAGAAGGCAAAGAAAATGATTGGTCTGAAG GCTAAGCTTTACCATAAACAGCGTCATGCTGAGAAAATACAAATGAAAAAGACTATCAAGATGCATGAA AAGAGAAACACCAAACAAAAGAATGATGAAAAGACACCACAGGGAGCAGTACCTGCCTATCTGCTGGAC AGAGAGGGACAATCTCGAGCTAAAGTACTTTCCAATATGATTAAACAGAAAAGAAAAGAGAAGGCGGGA AAATGGGAAGTCCCTCTGCCTAAAGTACGTGCCCAGGGAGAAACAGAAGTATTAAAAGTTATTCGAACA GGAAAGAGAAAGAAGAAGGCATGGAAGAGAATGGTTACTAAAGTGTGCTTTGTTGGAGATGGCTTTACA AGAAAACCACCTAAATATGAAAGATTCATCAGGCCAATGGAAAATATGCCCAGGTTACCAACAATCCTG AAAATGATGGATGTATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271665
Insert Size	570 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271665.1
RefSeq Size	1208 bp
RefSeq ORF	570 bp
Locus ID	10412
UniProt ID	O95478
MW	22.7 kDa
Gene Summary	This gene encodes a nucleolar protein involved in cell cycle regulation and proliferation. This gene was identified based on sequence similarity to a highly conserved Saccharomyces cerevisiae gene encoding a pre-ribosomal protein, which is involved in large ribosomal subunit biogenesis. The encoded protein is found at elevated levels in diabetic nephropathy. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified. [provided by RefSeq, Nov 2012] Transcript Variant: This variant (2) lacks an exon in the coding region, which results in a frameshift and an early stop codon, compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1.

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