FAM113A (PCED1A) (NM_001271168) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bA12M19.1; C20orf81; FAM113A
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333040 representing NM_001271168. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGTCTTCTGTCTGTCGAGCGAGGAGCCGCGCCGCCCGCTGCGAAGCGACATGGTCCACTTCCAGGCC TCGGAAGTCCAGCAGCTGCTACACAACAAGTTCGTGGTCATCTTGGGGGACTCCATTCAGAGGGCTGTG TACAAGGACCTGGTGCTCTTGCTCCAGAAAGACTCACTGCTCACAGCTGCCCAGCTGAAAGCCAAGTAC CTTGAGGATGTTCTGGAAGAGCTGACATATGGACCTGCCCCGGACCTGGTGATCATCAACTCCTGCCTC TGGGATCTCTCCAGATATGGTCGCTGCTCAATGGAGAGCTACCGGGAGAACCTGGAGCGGGTGTTTGTG CGCATGGACCAAGTATTGCCAGACTCCTGCCTGCTGGTGTGGAACATGGCGATGCCCCTCGGGGAACGT ATCACTGGGGGTTTCCTCCTGCCAGAGCTCCAGCCCCTGGCAGGCTCCCTGCGGCGGGATGTGGTTGAA GGGAACTTCTACAGTGCTACGCTGGCCGGGGACCACTGCTTTGATGTCCTAGACCTCCACTTTCACTTC CGGCATGCAGTACAGCACCGTCATCGGGATGGTGTCCACTGGGACCAGCATGCACACCGCCACCTCTCA CACCTGCTTCTGACCCATGTGGCTGACGCCTGGGGCGTGGAGCTGCCCAAGCGTGGCTATCCCCCTGAC CCGTGGATTGAGGACTGGGCAGAGATGAATCATCCATTCCAGGGAAGCCATAGGCAGACCCCAGACTTC GGGGAGCACCTGGCCTTGCTCCCACCCCCACCTTCTTCTTTGCCTCCTCCCATGCCTTTTCCCTACCCG CTTCCTCAGCCCTCGCCACCTCCCCTCTTCCCACCCCTGCCCCAGGATACCCCTTTTTTCCCAGGCCAG CCCTTCCCACCCCATGAATTCTTCAACTATAATCCAGTGGAGGACTTCTCGATGCCACCCCACTTAGGA TGTGGCCCTGGAGTGAACTTTGTGCCTGGCCCTCTGCCACCTCCAATCCCTGGCCCTAATCCCCATGGT CAGCACTGGGGCCCAGTGGTCCACCGGGGGATGCCACGCTATGTTCCTAACAGCCCCTACCATGTGCGG AGAATGGGGGGGCCCTGCAGGCAGCGGCTCAGACACTCAGAGAGACTGATCCACACATACAAACTGGAC AGACGGCCTCCTGCCCATTCGGGGACATGGCCTGGGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001271168
Insert Size	1212 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271168.1
RefSeq Size	1774 bp
RefSeq ORF	1212 bp
Locus ID	64773
UniProt ID	Q9H1Q7
MW	46 kDa
Gene Summary	The protein encoded by this gene is a member of the GDSL/SGNH superfamily. Members of this family are hydrolytic enzymes with esterase and lipase activity and broad substrate specificity. This protein belongs to the Pmr5-Cas1p-esterase subfamily in that it contains the catalytic triad comprised of serine, aspartate and histidine and lacks two conserved regions (glycine after strand S2 and GxND motif). A pseudogene of this gene has been identified on the long arm of chromosome 2. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Sep 2012] Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate in-frame splice site in the coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1.

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