MARCHF6 (NM_001270661) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DOA10; FAME3; MARCH-VI; MARCH6; RNF176; TEB4
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332991 representing NM_001270661. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGACACCGCGGAGGAAGGCCGCATCTACAAGTGCTTGTTTACTGGCTCCGTGAGCTCACTACTGACG CTGCCATTAGATATGCTGTCAACGGAAAATTTGTTGGCAGATTGTTTGCAGGGTTGTTTTGTGGTGACG TGCACACTGTGTGCATTCATCAGCCTGGTGTGGTTGAGAGAGCAGATAGTCCATGGGGGAGCACCAATT TGGTTGGAGCATGCTGCCCCACCGTTCAATGCTGCGGGGCATCACCAAAATGAGGCTCCAGCAGGAGGA AATGGTGCAGAAAATGTTGCTGCTGATCAGCCTGCTAACCCACCAGCTGAGAACGCAGTGGTGGGGGAA AACCCTGATGCCCAGGATGACCAGGCAGAAGAGGAGGAGGAGGACAATGAGGAGGAAGATGACGCTGGT GTGGAGGATGCGGCAGATGCTAATAACGGAGCCCAGGATGACATGAATTGGAATGCTTTAGAATGGGAC CGAGCTGCTGAAGAGCTTACATGGGAAAGAATGCTAGGACTTGATGGATCACTAGTTTTTCTGGAACAT GTCTTCTGGGTGGTATCTTTAAATACACTGTTCATTCTTGTTTTTGCATTTTGCCCTTACCATATTGGT CATTTCTCCCTTGTTGGTTTGGGATTTGAAGAACACGTCCAAGCATCTCATTTTGAAGGCCTAATCACA ACCATAGTTGGGTATATACTTTTAGCAATAACACTGATAATTTGTCATGGCTTGGCAACTCTTGTGAAA TTTCATAGATCTCGTCGCTTACTGGGAGTCTGCTATATTGTTGTTAAGGTCTCTTTGTTAGTGGTGGTA GAAATTGGAGTATTCCCTCTCATTTGTGGTTGGTGGCTGGATATCTGTTCCTTGGAAATGTTTGATGCT ACTCTGAAAGATCGAGAACTGAGCTTTCAGTCGGCTCCAGGTACTACCATGTTTCTGCATTGGCTAGTG GGAATGGTATATGTCTTCTACTTTGCCTCCTTCATTCTACTACTGAGAGAGGTACTTCGACCTGGTGTC CTGTGGTTTCTAAGGAATTTGAATGATCCAGATTTCAATCCAGTACAGGAAATGATCCATTTGCCAATA TATAGGCATCTCCGAAGATTTATTTTGTCAGTGATTGTCTTTGGCTCCATTGTCCTCCTGATGCTTTGG CTTCCTATACGTATAATTAAGAGTGTGCTGCCTAATTTTCTTCCATACAATGTCATGCTCTACAGTGAT GCTCCAGTGAGTGAACTGTCCCTCGAGCTGCTTCTGCTTCAGGTTGTCTTGCCAGCATTACTCGAACAG GGACACACGAGGCAGTGGCTGAAGGGGCTGGTGCGAGCGTGGACTGTGACCGCCGGATACTTGCTGGAT CTTCATTCTTATTTATTGGGAGACCAGGAAGAAAATGAAAACAGTGCAAATCAACAAGTTAACAATAAT CAGCATGCTCGAAATAACAACGCTATTCCTGTGGTGGGAGAAGGCCTTCATGCAGCCCACCAAGCCATA CTCCAGCAGGGAGGGCCTGTTGGCTTTCAGCCTTACCGCCGACCTTTAAATTTTCCACTCAGGATATTT CTGTTGATTGTCTTCATGTGTATAACATTACTGATTGCCAGCCTCATCTGCCTTACTTTACCAGTATTT GCTGGCCGTTGGTTAATGTCGTTTTGGACGGGGACTGCCAAAATCCATGAGCTCTACACAGCTGCTTGT GGTCTCTATGTTTGCTGGCTAACCATAAGGGCTGTGACGGTGATGGTGGCATGGATGCCTCAGGGACGC AGAGTGATCTTCCAGAAGGTTAAAGAGTGGTCTCTCATGATCATGAAGACTTTGATAGTTGCGGTGCTG TTGGCTGGAGTTGTCCCTCTCCTTCTGGGGCTCCTGTTTGAGCTGGTCATTGTGGCTCCCCTGAGGGTT CCCTTGGATCAGACTCCTCTTTTTTATCCATGGCAGGACTGGGCACTTGGAGTCCTGCATGCCAAAATC ATTGCAGCTATAACATTGATGGGTCCTCAGTGGTGGTTGAAAACTGTAATTGAACAGGTTTACGCAAAT GGCATCCGGAACATTGACCTTCACTATATTGTTCGTAAACTGGCAGCTCCCGTGATCTCTGTGCTGTTG CTTTCCCTGTGTGTACCTTATGTCATAGCTTCTGGTGTTGTTCCTTTACTAGGTGTTACTGCGGAAATG CAAAACTTAGTCCATCGGCGGATTTATCCATTTTTACTGATGGTCGTGGTATTGATGGCAATTTTGTCC TTCCAAGTCCGCCAGTTTAAGCGCCTTTATGAACATATTAAAAATGACAAGTACCTTGTGGGTCAACGA CTCGTGAACTACGAACGGAAATCTGGCAAACAAGGCTCATCTCCACCACCTCCACAGTCATCCCAAGAA TAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270661
Insert Size	2418 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270661.1
RefSeq Size	9395 bp
RefSeq ORF	2418 bp
Locus ID	10299
UniProt ID	O60337
Protein Families	Transmembrane
MW	90.4 kDa
Gene Summary	This gene encodes a member of a family of membrane-associated E3 ubiquitin ligases containing RING-CH-type zinc finger motifs. Ubiquitination of type II deiodinase by the encoded protein is an important regulatory step in thyroid hormone signalling. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012] Transcript Variant: This variant (3) lacks three alternate in-frame exons in the coding region, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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