SRP72 (NM_001267722) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BMFF; BMFS1; HEL103
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332875 representing NM_001267722. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCGAGCGGCGGCAGCGGGGGGGTGTCAGTACCTGCGCTGTGGAGTGAAGTGAACCGGTATGGCCAG AACGGCGACTTCACGCGCGCTCTCAAGACCGTCAATAAGATACTACAGATCAACAAAGATGACGTAACT GCCCTGCATTGTAAAGTGGTATGCCTTATCCAGAATGGAAGTTTCAAGGAAGCTTTGAATGTCATCAAT ACTCACACCAAAGTGTTAGCCAATAACTCTCTCTCCTTTGAAAAGGCATATTGCGAGTACAGGCTGAAC AGAATTGAGAATGCCTTGAAGACAATAGAAAGTGCCAACCAGCAGACAGACAAACTGAAGGAGCTTTAT GGACAAGTGTTATACCGTTTGGAACGCTATGATGAATGCTTAGCAGTGTATAGAGATCTCGTCCGAAAC TCCCAAGATGATTATGATGAGGAGAGGAAAACAAACCTTTCAGCAGTTGTTGCAGCTCAAAGCAATTGG GAAAAAGTGGTTCCAGAGAACCTGGGCCTCCAAGAAGGCACACATGAGCTGTGCTACAACACTGCATGT GCACTGATAGGCCAAGGCCAGCTGAACCAGGCCATGAAAATCCTACAAAAAGCTGAAGATCTTTGCCGC CGTTCATTATCAGAAGACACTGACCAAAATGTCTTTGACTCCAAGAAGAAAGTGAAATTAACCAATGCG GAAGGAGTAGAGTTTAAGCTTTCCAAGAAACAACTACAAGCTATAGAATTTAACAAAGCTTTACTTGCT ATGTACACAAACCAGGCTGAACAATGCCGCAAAATATCTGCCAGTTTACAGTCCCAAAGTCCCGAGCAT CTCTTACCTGTGTTAATCCAAGCTGCCCAGCTCTGCCGTGAAAAGCAGCACACAAAAGCAATAGAGCTG CTTCAGGAATTTTCAGATCAGCATCCAGAAAATGCAGCTGAAATTAAGCTGACCATGGCACAGTTGAAA ATTTCTCAAGGTAATATTTCTAAAGCATGTCTAATATTGAGAAGCATAGAGGAGTTAAAGCATAAACCA GGCATGGTATCTGCATTAGTTACCATGTATAGCCATGAAGAAGATATTGATAGTGCCATTGAGGTCTTC ACACAAGCTATCCAGTGGTATCAAAACCATCAGCCAAAATCTCCTGCTCATTTGTCCTTGATAAGAGAA GCTGCAAACTTCAAACTCAAATATGGGCGGAAGAAGGAGGCAATTAGTGACCTACAACAGCTGTGGAAA CAAAATCCAAAAGATATTCACACCCTGGCACAGCTTATTTCTGCTTACTCACTTGTAGATCCAGAGAAA GCCAAAGCTCTTAGTAAACACTTGCCATCGTCAGATAGTATGTCTCTAAAAGTAGATGTTGAGGCTCTT GAAAATTCTGCTGGTGCTACATACATTCGGAAGAAGGGTGGAAAAGTTACTGGAGATAGTCAACCAAAG GAACAAGGACAGGGAGATTTGAAAAAGAAGAAAAAGAAAAAGAAGGGAAAATTGCCTAAGAATTATGAC CCAAAAGTTACCCCAGATCCAGAAAGATGGCTGCCAATGCGAGAACGTTCTTACTACCGGGGAAGAAAG AAGGGTAAAAAGAAGGATCAGATTGGAAAAGGGACCCAGGGAGCAACTGCAGGAGCTTCATCTGAACTG GATGCCAGTAAAACTGTGAGCAGCCCACCCACCTCCCCAAGACCTGGCAGTGCTGCAACAGTATCTGCC TCTACAAGTAACATCATACCCCCAAGACACCAGAAACCTGCAGGGGCTCCAGCAACAAAAAAGAAACAG CAACAGAAAAAGAAGAAAGGTGGAAAAGGTGGCTGGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001267722
Insert Size	1833 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001267722.1
RefSeq Size	3710 bp
RefSeq ORF	1833 bp
Locus ID	6731
UniProt ID	O76094
Protein Pathways	Protein export
MW	67.9 kDa
Gene Summary	This gene encodes the 72 kDa subunit of the signal recognition particle (SRP), a ribonucleoprotein complex that mediates the targeting of secretory proteins to the endoplasmic reticulum (ER). The SRP complex consists of a 7S RNA and 6 protein subunits: SRP9, SRP14, SRP19, SRP54, SRP68, and SRP72, that are bound to the 7S RNA as monomers or heterodimers. SRP has at least 3 distinct functions that can be associated with the protein subunits: signal recognition, translational arrest, and ER membrane targeting by interaction with the docking protein. Mutations in this gene are associated with familial bone marrow failure. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012] Transcript Variant: This variant (2) lacks 2 consecutive in-frame coding exons in the 5' region compared to variant 1, which results in a shorter isoform (2) missing an internal protein segment compared to isoform 1.

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