PRC1 (NM_001267580) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ASE1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332860 representing NM_001267580. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAGGAGAAGTGAGGTGCTGGCGGAGGAGTCCATAGTATGTCTGCAGAAAGCCCTAAATCACCTTCGG GAAATATGGGAGCTAATTGGGATTCCAGAGGACCAGCGGTTACAAAGAACTGAGGTGGTAAAGAAGCAT ATCAAGGAAGAAGGAGAGACGACCATCTTGCAACTAGAAAAAGATTTGCGCACCCAAGTGGAATTGATG CGAAAACAGAAAAAGGAGAGAAAACAGGAACTGAAGCTACTTCAAGAGCAAGATCAAGAACTGTGCGAA ATTCTTTGTATGCCCCACTATGATATTGACAGTGCCTCAGTGCCCAGCTTAGAAGAGCTGAACCAGTTC AGGCAACATGTGACAACTTTGAGGGAAACAAAGGCTTCTAGGCGTGAGGAGTTTGTCAGTATAAAGAGA CAGATCATACTGTGTATGGAAGAATTAGACCACACCCCAGACACAAGCTTTGAAAGAGATGTGGTGTGT GAAGACGAAGATGCCTTTTGTTTGTCTTTGGAGAATATTGCAACACTACAAAAGTTGCTACGGCAGCTG GAAATGCAGAAATCACAAAATGAAGCAGTGTGTGAGGGGCTGCGTACTCAAATCCGAGAGCTCTGGGAC AGGTTGCAAATACCTGAAGAAGAAAGAGAAGCTGTGGCCACCATTATGTCTGGGTCAAAGGCCAAGGTC CGGAAAGCGCTGCAATTAGAAGTGGATCGGTTGGAAGAACTGAAAATGCAAAACATGAAGAAAGTGATT GAGGCAATTCGAGTGGAGCTGGTTCAGTACTGGGACCAGTGCTTTTATAGCCAGGAGCAGAGACAAGCT TTTGCCCCTTTCTGTGCTGAGGACTACACAGAAAGTCTGCTCCAGCTCCACGATGCTGAGATTGTGCGG TTAAAAAACTACTATGAAGTTCACAAGGAACTCTTTGAAGGTGTCCAGAAGTGGGAAGAAACCTGGAGG CTTTTCTTAGAGTTTGAGAGAAAAGCTTCAGATCCAAATCGATTTACAAACCGAGGAGGAAATCTTCTA AAAGAAGAAAAACAACGAGCCAAGCTCCAGAAAATGCTGCCCAAGCTGGAAGAAGAGTTGAAGGCACGA ATTGAATTGTGGGAACAGGAACATTCAAAGGCATTTATGGTGAATGGGCAGAAATTCATGGAGTATGTG GCAGAACAATGGGAGATGCATCGATTGGAGAAAGAGAGAGCCAAGCAGGAAAGACAACTGAAGAACAAA AAACAGACAGAGACAGAGATGCTGTATGGCAGCGCTCCTCGAACACCTAGCAAGCGGCGAGGACTGGCT CCCAATACACCGGGCAAAGCACGTAAGCTGAACACTACCACCATGTCCAATGCTACGGCCAATAGTAGC ATTCGGCCTATCTTTGGAGGGACAGTCTACCACTCCCCCGTGTCTCGACTTCCTCCTTCTGGCAGCAAG CCAGTCGCTGCTTCCACCTGTTCAGGGAAGAAAACACCCCGTACTGGCAGGCATGGAGCCAACAAGGAG AACCTGGAGCTCAACGGCAGCATCCTGAGTGCGAGAACTTTCAAAGGCTTCCAAATCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001267580
Insert Size	1578 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001267580.1
RefSeq Size	2965 bp
RefSeq ORF	1578 bp
Locus ID	9055
UniProt ID	O43663
MW	61.4 kDa
Gene Summary	This gene encodes a protein that is involved in cytokinesis. The protein is present at high levels during the S and G2/M phases of mitosis but its levels drop dramatically when the cell exits mitosis and enters the G1 phase. It is located in the nucleus during interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). It is necessary for polarizing parallel microtubules and concentrating the factors responsible for contractile ring assembly. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012] Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 5' coding region, and uses and alternate splice site and lacks an exon that results in a frameshift in the 3' coding region, compared to variant 1. The encoded isoform (4) has a distinct C-terminus and is shorter than isoform 1.

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