JARID2 (NM_001267040) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	JMJ
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332835 representing NM_001267040. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGTGTATTTTGGAAGCTCTCAGGATGAGGAGGAAGTCGAGGAGGAAGATGATGAGACAGAAGACGTC AAAACAGCCACCAACAATGCTTCATCTTCATGCCAGTCGACCCCCAGGAAAGGAAAAACCCACAAACAT GTTCACAACGGGCATGTTTTCAATGGTTCCAGCAGGTCAACACGGGAGAAGGAACCTGTTCAAAAACAC AAAAGCAAAGAGGCCACTCCCGCAAAGGAGAAGCACAGCGATCACCGGGCTGACAGCCGCCGGGAGCAG GCTTCAGCTAACCACCCCGCAGCGGCCCCCTCCACGGGTTCCTCGGCCAAGGGGCTTGCTGCCACCCAT CACCACCCCCCTCTGCATCGGTCGGCTCAGGACTTACGGAAACAGGTTTCTAAGGTAAACGGAGTCACT CGAATGTCATCTCTGGGTGCAGGTGTAACCAGTGCCAAAAAGATGCGCGAGGTCAGACCTTCACCATCC AAAACTGTGAAGTACACTGCCACGGTGACGAAGGGGGCTGTCACATACACCAAAGCCAAGAGAGAACTG GTCAAGGACACCAAACCCAATCACCACAAGCCCAGTTCCGCTGTCAACCACACAATCTCAGGGAAAACT GAAAGTAGCAATGCAAAAACCCGCAAACAGGTGCTATCCCTCGGGGGGGCGTCCAAGTCCACTGGGCCC GCCGTCAATGGCCTCAAGGTCAGTGGCAGGTTGAACCCAAAGTCATGCACTAAGGAGGTGGGGGGGCGG CAGCTGCGGGAGGGCCTGCAGCTGCGGGAGGGGCTGCGGAACTCCAAGAGGAGACTGGAAGAGGCACAC CAGGCGGAGAAGCCGCAGTCGCCCCCCAAGAAGATGAAAGGGGCGGCTGGCCCCGCCGAAGGCCCTGGC AAGAAGGCCCCGGCCGAGAGAGGTCTGCTGAACGGACACGTGAAGAAGGAAGTGCCGGAGCGCAGTCTG GAGAGGAATCGGCCGAAGCGGGCCACGGCCGGGAAGAGCACGCCAGGCAGACAAGCACATGGCAAGGCG GACAGCGCCTCCTGTGAAAATCGTTCTACCTCGCAACCGGAGTCCGTGCACAAGCCGCAGGACTCGGGC AAGGCCGAGAAGGGCGGCGGCAAGGCCGGGTGGGCGGCCATGGACGAGATCCCCGTCCTCAGGCCCTCC GCCAAGGAGTTCCACGATCCGCTCATCTACATCGAGTCGGTCCGCGCTCAGGTGGAGAAGTTCGGGATG TGCAGGGTGATCCCCCCTCCGGACTGGCGGCCCGAGTGCAAGCTCAACGATGAGATGCGGTTTGTCACG CAGATTCAGCACATCCACAAGCTGGGCCGGCGCTGGGGCCCCAACGTGCAGCGGCTGGCCTGCATCAAG AAGCACCTCAAATCTCAGGGCATCACCATGGACGAGCTCCCGCTCATAGGGGGCTGTGAGCTCGACCTG GCCTGCTTTTTCCGGCTGATTAATGAGATGGGCGGCATGCAGCAAGTGACTGACCTCAAAAAATGGAAC AAACTAGCAGACATGCTGCGCATCCCCAGAACTGCCCAGGACCGGCTGGCCAAGCTGCAGGAGGCCTAC TGCCAGTACCTACTCTCCTACGACTCCCTGTCCCCAGAGGAGCACCGGCGGCTGGAGAAGGAGGTGCTG ATGGAGAAGGAGATCCTGGAGAAGCGCAAGGGGCCGCTGGAAGGCCACACAGAGAACGACCACCACAAG TTCCACCCTCTGCCCCGCTTCGAGCCCAAGAATGGGCTCATCCACGGCGTGGCCCCCAGGAACGGCTTC CGCAGCAAGCTCAAGGAGGTGGGCCAGGCCCAGTTGAAGACTGGCCGGCGGCGACTCTTCGCTCAGGAA AAAGAAGTGGTCAAGGAAGAGGAGGAGGACAAAGGCGTCCTCAATGACTTCCACAAGTGCATCTATAAG GGAAGGTCTGTTTCTCTAACAACTTTTTATCGAACAGCGAGGAATATCATGAGCATGTGTTTCAGCAAG GAGCCTGCCCCAGCCGAAATCGAGCAAGAGTACTGGAGGCTAGTGGAAGAGAAGGACTGCCACGTGGCA GTGCACTGCGGCAAGGTGGACACCAACACTCACGGCAGTGGATTCCCAGTAGGAAAATCAGAACCCTTT TCGAGGCATGGATGGAACCTCACCGTCCTCCCCAATAACACAGGGTCCATCCTGCGTCACCTCGGTGCT GTGCCTGGAGTGACTATTCCCTGGCTAAATATTGGCATGGTCTTTTCTACCTCATGCTGGTCTCGAGAC CAAAATCACCTTCCATACATTGACTACTTACACACTGGTGCTGACTGCATTTGGTATTGCATTCCTGCT GAGGAGGAGAACAAGCTGGAAGATGTGGTCCACACCCTGCTGCAAGCCAATGGCACCCCAGGGCTGCAG ATGCTGGAAAGCAACGTCATGATCTCCCCGGAGGTGCTGTGCAAAGAGGGGATCAAGGTGCACAGGACC GTGCAGCAGAGTGGCCAGTTTGTCGTCTGCTTCCCGGGATCCTTTGTGTCCAAAGTGTGCTGTGGGTAC AGCGTGTCTGAAACCGTGCACTTTGCTACCACCCAGTGGACAAGTATGGGCTTTGAGACCGCCAAGGAA ATGAAGCGTCGCCATATAGCTAAGCCATTCTCCATGGAGAAGTTACTCTACCAGATTGCACAAGCAGAA GCAAAAAAAGAAAACGGTCCCACTCTCAGTACCATCTCAGCCCTCCTGGATGAGCTCAGGGATACAGAG CTGCGGCAGCGCAGGCAGCTGTTCGAGGCTGGCCTCCACTCCTCCGCACGCTATGGCAGCCACGATGGC AGCAGCACGGTGGCGGACGGGAAGAAAAAGCCTCGAAAGTGGCTGCAGTTGGAGACGTCAGAGAGGAGG TGTCAGATCTGCCAGCACCTGTGCTACCTGTCCATGGTGGTACAAGAGAACGAAAACGTCGTGTTCTGT CTGGAGTGTGCTCTGCGCCACGTGGAGAAACAGAAGTCCTGCCGAGGGCTGAAGTTGATGTACCGCTAC GATGAGGAACAGATTATCAGTCTGGTCAATCAGATCTGCGGCAAAGTGTCTGGTAAAAACGGCAGCATT GAGAACTGTCTCAGTAAACCCACACCAAAAAGAGGTCCCCGCAAGAGAGCGACAGTGGACGTGCCCCCC TCCCGTCTGTCAGCCTCCAGTTCATCCAAAAGTGCTTCGAGCTCATCATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001267040
Insert Size	3225 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001267040.1
RefSeq Size	5612 bp
RefSeq ORF	3225 bp
Locus ID	3720
UniProt ID	Q92833
Protein Families	Transcription Factors
MW	119.7 kDa
Gene Summary	This gene encodes a Jumonji- and AT-rich interaction domain (ARID)-domain-containing protein. The encoded protein is a DNA-binding protein that functions as a transcriptional repressor. This protein interacts with the Polycomb repressive complex 2 (PRC2) which plays an essential role in regulating gene expression during embryonic development. This protein facilitates the recruitment of the PRC2 complex to target genes. Alternate splicing results in multiple transcript variants. Mutations in this gene are associated with chronic myeloid malignancies. [provided by RefSeq, May 2012] Transcript Variant: This variant (2) differs in the 5' UTR and initiates translation at a downstream AUG, compared to variant 1. The resulting isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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