SRP68 (NM_001260502) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332745 representing NM_001260502. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCTGCTGAGAAGCAGGTCCCAGGCGGCGGCGGCGGCGGCGGCAGTGGCGGCGGCGGTGGCAGTGGC GGCGGCGGTAGCGGCGGTGGACGTGGTGCCGGAGGGGAAGAAAATAAAGAAAACGAACGCCCTTCGGCC GGATCGAAGGCAAACAAAGAATTTGGGGATAGCCTGAGTTTGGAGATTCTTCAGATTATTAAGGAATCC CAGCAGCAGCATGGTTTACGGCATGGAGATTTTCAGAGGTACAGATACTTGCTTCTGGTTCTGATGGAT GCTGAAAGAGCCTGGAGCTACGCCATGCAGCTGAAACAGGAAGCCAACACTGAACCCCGAAAACGGTTT CACTTGTTATCTCGCCTACGCAAAGCCGTGAAGCATGCAGAGGAATTGGAACGCTTGTGTGAGAGCAAT CGCGTGGATGCCAAGACCAAATTAGAGGCTCAGGCTTACACAGCTTACCTCTCAGGAATGCTACGTTTT GAACATCAAGAATGGAAAGCTGCCATTGAGGCTTTTAACAAATGCAAAACTATCTATGAGAAGCTAGCC AGTGCTTTCACAGAGGAGCAGGCTGTGCTGTATAACCAACGTGTGGAAGAGATTTCACCCAACATCCGC TATTGTGCATATAATATTGGGGACCAGTCAGCCATCAATGAACTCATGCAGATGAGATTGAGGTCTGGG GGCACTGAGGGTCTCTTGGCTGAAAAATTGGAGGCTTTGATCACTCAGACTCGAGCCAAACAGGCAGCT ACCATGAGTGAAGTGGAGTGGAGAGGGAGAACGGTTCCAGTGAAGATTGACAAAGTGCGCATTTTCTTA TTAGGACTGGCTGATAACGAAGCAGCTATTGTCCAGGCTGAAAGCGAAGAAACTAAGGAGCGCCTGTTT GAATCAATGCTCAGCGAGTGTCGGGACGCCATCCAGGTGGTTCGGGAGGAGCTCAAGCCAGATCAGAAA CAGAGAGATTATATCCTTGAAGGAGAGCCAGGGAAGGTGTCTAATCTTCAATACTTGCATAGCTACCTG ACTTACATCAAGCTATCAACGGCAATCAAGCGTAATGAGAACATGGCCAAAGGTCTGCAGAGGGCTCTG CTGCAGCAGCAGCCAGAGGATGACAGCAAGCGCTCACCCCGGCCCCAGGACCTGATCCGACTCTATGAC ATCATCTTACAGAATCTGGTGGAATTGCTCCAGCTTCCTGGTTTAGAGGAAGACAAAGCCTTCCAGAAA GAGATAGGCCTCAAGACTCTGGTGTTCAAAGCTTACAGGTGTTTTTTCATTGCTCAGTCCTATGTGCTG GTGAAGAAGTGGAGCGAAGCCCTTGTCCTGTATGACAGAGTCCTGAAATATGCAAATGAAGTAAATTCT GATGCTGGCGCCTTCAAGAACAGCCTAAAGGACCTGCCTGATGTGCAAGAGCTCATCACTCAAGTGCGG TCAGAGAAGTGCTCCCTGCAGGCCGCAGCCATCCTTGATGCAAACGACGCTCATCAAACAGAGACCTCC TCCTCCCAAGTCAAGGACAATAAGCCTCTGGTTGAACGGTTTGAGACATTCTGCCTGGACCCTTCCCTT GTCACCAAGCAAGCCAACCTTGTGCACTTCCCACCAGGCTTCCAGCCCATTCCCTGCAAGCCTTTGTTC TTTGACCTGGCCCTCAACCATGTGGCTTTCCCACCCCTTGAGGACAAGTTGGAACAGAAGACCAAGAGT GGCCTCACTGGATACATCAAGGGCATCTTTGGATTCAGGAGCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001260502
Insert Size	1770 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001260502.1
RefSeq Size	2736 bp
RefSeq ORF	1770 bp
Locus ID	6730
UniProt ID	Q9UHB9
Protein Pathways	Protein export
MW	66.1 kDa
Gene Summary	This gene encodes a subunit of the signal recognition particle (SRP). The SRP is a ribonucleoprotein complex that transports secreted and membrane proteins to the endoplasmic reticulum for processing. The complex includes a 7S RNA and six protein subunits. The encoded protein is the 68kDa component of the SRP, and forms a heterodimer with the 72kDa subunit that is required for SRP function. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and three pseudogenes of this gene are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, May 2012] Transcript Variant: This variant (2) lacks an exon in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

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