PKD2L2 (NM_001258448) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	TRPP5
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332715 representing NM_001258448. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCTGAGGCGTCACGGTGGCACCGAGGCGGGGCTTCGAAACATAAGTTGCATTACAGAAAGGAAGTA GAAATTACAACCACACTTCAGGAATTGTTACTCTACTTTATTTTTTTAATAAACCTATGTATATTGACT TTTGGGATGGTAAACCCACATATGTATTACTTAAACAAGGTTATGTCATCTCTATTTTTGGACACTTCT GTGCCTGGTGAAGAAAGAACCAACTTTAAGTCCATTCGCAGCATAACTGATTTTTGGAAGTTTATGGAA GGACCCCTTTTGGAAGGTCTGTACTGGGATTCATGGTACAATAACCAGCAGCTGTATAATTTAAAGAAC AGCAGTCGCATCTACTATGAAAATATACTTCTAGGAGTTCCCAGAGTTCGTCAACTAAAAGTCCGCAAC AACACATGCAAAGTCTATTCATCTTTTCAGTCTTTGATGAGTGAATGTTATGGCAAATATACTTCTGCA AATGAAGACCTCTCTAATTTTGGCCTTCAAATTAATACTGAATGGAGATATTCTACTTCTAATACCAAC TCCCCTTGGCACTGGGGATTTCTTGGTGTTTACCGAAATGGGGGATACATTTTCACTTTATCAAAATCG AAATCTGAAACCAAAAACAAGTTCATTGACCTTCGACTGAACAGCTGGATCACAAGAGGGACTAGAGTT ATTTTTATTGATTTTTCCTTATATAATGCTAATGTAAATCTATTTTGTATTATCAGATTGGTGGCAGAA TTCCCTGCAACTGGAGGAATACTTACTTCATGGCAGTTTTACTCTGTGAAGCTCCTCAGATATGTTAGC TACTATGACTATTTTATTGCTTCCTGTGAAATCACATTCTGTATTTTTCTTTTTGTCTTCACAACACAA GAAGTCAAAAAAATAAAAGAATTTAAGTCTGCCTATTTCAAAAGTATTTGGAACTGGCTAGAATTGCTA CTTTTGCTGCTGTTGAAAAGTACTGAAAAATATTCAGATTTCTATTTTCTTGCATGCTGGCACATTTAT TACAATAATATAATTGCTATTACCATCTTTTTTGCATGGATAAAGATATTCAAATTCATAAGCTTTAAC AAGACAATGTCTCAGCTGTCATCAACCTTGTCCCGTTGTGTTAAAGACATAGTAGGATTTGCCATCATG TTTTTTATAATATTCTTTGCTTATGCCCAGTTAGGATTTCTTGTTTTTGGATCACAAGTTGATGACTTT TCCACTTTTCAGAATTCCATATTTGCACAATTTCGAATTGTTCTTGGAGATTTTAATTTTGCTGGTATT CAGCAAGCCAATCCTATCTTGGGACCCATTTACTTCATCACTTTCATCTTTTTTGTGTTCTTTGTCCTG CTGAATATGTTCTTGGCAATTATTAATGATACCTATTCTGAAGTGAAAGCTGACTATTCAATAGGCAGA AGGCTAGATTTTGAACTTGGCAAAATGATTAAACAGAGTTACAAAAATGTTCTCGAGAAATTCAGACTG AAGAAAGCTCAAAAAGATGAAGACAAGAAAACCAAAGGCAGCGGAGATTTGGCTGAACAAGCCAGAAGA GAAGGCTTTGACGAAAATGAGATTCAAAACGCAGAGCAGATGAAAAAATGGAAAGAGAGGCTTGAGAAA AAGTATTATTCTATGGAAATTCAAGATGACTACCAGCCTGTCACTCAAGAAGAATTTCGAGAACTTTTT TTATATGCTGTGGAGCTGGAGAAGGAATTACACTACATCAATTTGAAGCTAAATCAAGTGGTGAGAAAG GTTTCAGCTCTATAG
Restriction Sites	SgfI-MluI
ACCN	NM_001258448
Insert Size	1809 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001258448.1
RefSeq Size	2260 bp
RefSeq ORF	1809 bp
Locus ID	27039
UniProt ID	Q9NZM6
Protein Families	Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
MW	71.3 kDa
Gene Summary	May function as a subunit of a cation channel and play a role in fertilization.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has an alternate in-frame splice site in the central coding region and an additional exon in the 3' region, which results in an alternate translation stop codon, compared to variant 1. The resulting isoform (2) is shorter; it lacks an internal segment and has a distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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