NDRG1 (NM_001258432) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CAP43; CMT4D; DRG-1; DRG1; GC4; HMSNL; NDR1; NMSL; PROXY1; RIT42; RTP; TARG1; TDD5
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332706 representing NM_001258432. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAACCACAAAACCTGCTACAACCCCCTCTTCAACTACGAGGACATGCAGGAGATCACCCAGCACTTT GCCGTCTGCCACGTGGACGCCCCTGGCCAGCAGGACGGCGCAGCCTCCTTCCCCGCAGGGTACATGTAC CCCTCCATGGATCAGCTGGCTGAAATGCTTCCTGGAGTCCTTCAACAGTTTGGGCTGAAAAGCATTATT GGCATGGGAACAGGAGCAGGCGCCTACATCCTAACTCGATTTGCTCTAAACAACCCTGAGATGGTGGAG GGCCTTGTCCTTATCAACGTGAACCCTTGTGCGGAAGGCTGGATGGACTGGGCCGCCTCCAAGATCTCA GGATGGACCCAAGCTCTGCCGGACATGGTGGTGTCCCACCTTTTTGGGAAGGAAGAAATGCAGAGTAAC GTGGAAGTGGTCCACACCTACCGCCAGCACATTGTGAATGACATGAACCCCGGCAACCTGCACCTGTTC ATCAATGCCTACAACAGCCGGCGCGACCTGGAGATTGAGCGACCAATGCCGGGAACCCACACAGTCACC CTGCAGTGCCCTGCTCTGTTGGTGGTTGGGGACAGCTCGCCTGCAGTGGATGCCGTGGTGGAGTGCAAC TCAAAATTGGACCCAACAAAGACCACTCTCCTCAAGATGGCGGACTGTGGCGGCCTCCCGCAGATCTCC CAGCCGGCCAAGCTCGCTGAGGCCTTCAAGTACTTCGTGCAGGGCATGGGATACATGCCCTCGGCTAGC ATGACCCGCCTGATGCGGTCCCGCACAGCCTCTGGTTCCAGCGTCACTTCTCTGGATGGCACCCGCAGC CGCTCCCACACCAGCGAGGGCACCCGAAGCCGCTCCCACACCAGCGAGGGCACCCGCAGCCGCTCGCAC ACCAGCGAGGGGGCCCACCTGGACATCACCCCCAACTCGGGTGCTGCTGGGAACAGCGCCGGGCCCAAG TCCATGGAGGTCTCCTGCTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001258432
Insert Size	987 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001258432.1
RefSeq Size	3006 bp
RefSeq ORF	987 bp
Locus ID	10397
UniProt ID	Q92597
MW	35.5 kDa
Gene Summary	This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012] Transcript Variant: This variant (3) lacks two consecutive internal exons and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1.

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