EPS15R (EPS15L1) (NM_001258376) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EPS15R
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332681 representing NM_001258376. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCGGCGCCGCTCATCCCCCTCTCCCAGCAGATTCCCACTGGAAATTCGTTGTATGAATCTTATTAC AAGCAGGTCGATCCGGCATACACAGGGAGGGTGGGGGCGAGTGAAGCTGCGCTTTTTCTAAAGAAGTCT GGCCTCTCGGACATTATCCTTGGGAAGATATGGGACTTGGCCGATCCAGAAGGTAAAGGGTTCTTGGAC AAACAGGGTTTCTATGTTGCACTGAGACTGGTGGCCTGTGCACAGAGTGGCCATGAAGTTACCTTGAGC AATCTGAATTTGAGCATGCCACCGCCTAAATTTCACGACACCAGCAGCCCTCTGATGGTCACACCGCCC TCTGCAGAGGCCCACTGGGCTGTGAGGGTGGAAGAAAAGGCCAAATTTGATGGGATTTTTGAAAGCCTC TTGCCCATCAATGGTTTGCTCTCTGGAGACAAAGTCAAGCCAGTCCTCATGAACTCAAAGCTGCCTCTT GATGTCCTGGGCAGGGTCTGGGACCTCAGTGACATTGACAAGGATGGGCACTTGGATCGAGATGAGTTC GCTGTGGCCATGCACTTGGTGTACCGAGCCCTGGAGAAGGAGCCCGTGCCCTCCGCCCTGCCCCCGTCC CTCATCCCACCCTCCAAGAGAAAGAAGACTGTGTTCCCTGGCGCCGTCCCCGTCCTGCCTGCCAGCCCC CCACCAAAAGACAGCCTCCGCTCCACGCCGTCCCACGGCAGCGTCAGCAGCCTCAACAGCACAGGGAGC CTGTCCCCCAAGCACAGCCTCAAGCAAACACAGCCAACAGTGAACTGGGTGGTGCCCGTGGCAGACAAG ATGCGATTTGATGAGATATTCCTGAAGACCGACCTGGACCTGGATGGCTACGTGAGTGGCCAGGAGGTG AAGGAGATCTTCATGCACTCGGGCCTCACCCAGAACCTTCTAGCACACATATGGGCCCTGGCCGATACG AGGCAAACGGGGAAGTTAAGCAAAGACCAATTCGCGTTAGCTATGTATTTCATTCAGCAGAAGGTCAGT AAAGGCATCGACCCTCCTCAAGTCCTCTCGCCGGACATGGTCCCGCCTTCGGAGAGAGGCACGCCCGGC CCGGACAGTTCAGGCTCTCTCGGCTCCGGGGAGTTTACTGGCGTGAAGGAGCTTGATGACATCAGTCAA GAGATTGCCCAGTTACAAAGAGAGAAATATTCACTGGAACAAGACATTCGAGAAAAGGAAGAGGCAATC AGACAGAAAACCAGCGAGGTGCAGGAATTACAAAATGACCTAGACCGGGAAACAAGCAGTTTGCAGGAG CTCGAGGCTCAGAAACAGGATGCTCAAGACCGCCTGGACGAGATGGACCAGCAGAAGGCCAAGCTCCGA GACATGCTGAGCGACGTCCGGCAGAAGTGCCAGGATGAGACTCAGATGATCTCATCACTGAAAACGCAA ATCCAATCTCAGGAATCTGACTTAAAGTCCCAGGAAGACGATCTGAACCGAGCCAAGTCGGAGCTGAAC CGATTGCAGCAGGAGGAAACCCAGCTGGAGCAGAGCATTCAGGCTGGGCGAGTCCAGCTGGAAACCATC ATCAAGTCCCTGAAGTCAACGCAAGACGAAATCAACCAGGCAAGGAGCAAACTTTCCCAGCTGCATGAA AGCCGCCAGGAGGCCCACAGGAGCCTGGAGCAGTATGACCAGGTGCTCGATGGAGCCCATGGTGCCAGC CTGACCGACCTGGCCAACCTGAGCGAAGGCGTCTCCCTGGCAGAGAGGGGCAGTTTTGGAGCCATGGTA AAGGTTGAATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001258376
Insert Size	1806 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001258376.1
RefSeq Size	3566 bp
RefSeq ORF	1806 bp
Locus ID	58513
UniProt ID	Q9UBC2
Protein Families	Druggable Genome
MW	66.5 kDa
Gene Summary	Seems to be a constitutive component of clathrin-coated pits that is required for receptor-mediated endocytosis. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 3' UTR, compared to variant 1. The encoded isoform (4) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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