Histidase (HAL) (NM_001258334) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HIS; HSTD
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332667 representing NM_001258334. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCCAGATACACGGTGCACGTACGTGGGGAATGGCTGGCAGTGCCCTGCCAGGACGCGCAGCTCACT GTGGGCTGGCTGGGCCGGGAGGCCGTGAGGCGCTATATCAAGAATAAGCCCGACAATGGTGGCTTCACC TCCGTGGATGACGCGCACTTCCTTGTGCGCCGGTGCAAGGGCCTGGGCCTGCTGGACAACGAGGACCGG CTCGAGGTGGCCCTAGAGAACAACGAGTTCGTGGAAGTGGTTATAGAGGGTGATGCCATGTCTCCTGAC TTCATTCCATCTCAACCAGAAGGAGTTTATCTATACAGCAAGTACCGGGAGCCTGAAAAGTACATCGAG TTAGATGGAGACCGTCTGACCACGGAGGATCTGGTCAACTTGGGAAAGGGACGCTACAAAATAAAGCTC ACCCCAACAGCTGAGAAGAGGGTGCAGAAATCCAGGGAGGTCATAGATAGCATCATAAAAGAGAAAACA GTTGTTTACGGTATTACTACAGGTTTTGGGAAATTTGCCAGAACTGTAATTCCTATCAATAAGCTACAG GAGCTTCAGGTCAACTTAGTACGCTCACATTCTTCAGGTGTTGGGAAACCACTAAGTCCTGAGAGGTGT CGGATGCTCTTGGCTTTAAGGATCAATGTCTTAGCCAAAGGATACAGTGGCATTTCCCTGGAGACCCTC AAACAAGTCATAGAAATGTTTAATGCCTCCTGCCTGCCCTATGTCCCAGAGAAAGGAACCGTTGGTGCC AGTGGAGACCTTGCCCCACTCTCTCATCTTGCTCTTGGGCTAGTTGGAGAAGGGAAGATGTGGTCTCCG AAGAGTGGCTGGGCTGATGCTAAATACGTGCTAGAAGCCCATGGATTGAAACCAGTTATTTTAAAACCA AAAGAGGGCCTGGCACTCATCAATGGGACGCAGATGATCACATCCCTGGGCTGTGAAGCTGTAGAGCGA GCCAGTGCTATTGCACGGCAGGCTGACATTGTGGCAGCCCTGACCCTTGAGGTGCTGAAGGGCACCACC AAAGCCTTTGACACTGACATTCATGCTCTTCGACCTCACCGTGGGCAAATTGAAGTTGCTTTTCGGTTT CGGTCACTCTTGGACTCAGATCACCACCCATCAGAAATAGCAGAGAGTCACAGGTTCTGTGATCGCGTC CAGGATGCATACACCTTGCGCTGCTGTCCACAGGTCCATGGTGTGGTGAATGATACAATAGCATTTGTG AAGAACATCATTACCACAGAACTGAACAGCGCAACAGATAATCCTATGGTCTTTGCCAATAGGGGAGAG ACAGTTTCTGGAGGAAACTTCCATGGTGAATACCCAGCCAAAGCCCTAGACTACTTGGCCATTGGCATC CATGAACTTGCTGCAATCAGTGAGAGAAGAATCGAGCGGCTCTGCAATCCCTCCCTCAGTGAGCTGCCT GCCTTCCTGGTGGCTGAAGGTGGTCTGAACTCTGGGTTCATGATAGCTCACTGCACGGCAGCAGCCCTT GTTTCTGAGAACAAGGCTCTGTGCCATCCCTCGTCTGTTGACTCCCTCTCCACCAGCGCAGCCACGGAG GACCACGTCTCCATGGGAGGATGGGCAGCAAGGAAAGCCCTCAGGGTCATCGAGCATGTGGAGCAAGTG CTGGCCATCGAGCTCCTTGCAGCCTGCCAGGGCATAGAGTTTCTACGTCCCCTGAAAACAACCACTCCG CTGGAGAAGGTCTATGACCTGGTGCGCTCTGTTGTAAGGTTTGGGAAGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001258334
Insert Size	1776 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001258334.1
RefSeq Size	3857 bp
RefSeq ORF	1776 bp
Locus ID	3034
UniProt ID	P42357
Protein Families	Druggable Genome
Protein Pathways	Histidine metabolism, Metabolic pathways, Nitrogen metabolism
MW	64.9 kDa
Gene Summary	Histidine ammonia-lyase is a cytosolic enzyme catalyzing the first reaction in histidine catabolism, the nonoxidative deamination of L-histidine to trans-urocanic acid. Histidine ammonia-lyase defects cause histidinemia which is characterized by increased histidine and histamine and decreased urocanic acid in body fluids. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] Transcript Variant: This variant (3) lacks an alternate coding exon compared to variant 1, that causes a frameshift. The resulting isoform (3) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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