Gelsolin (GSN) (NM_001258029) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ADF; AGEL
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332582 representing NM_001258029. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCCGAGGAAGAAGCCCTCAGGGGCAATTCTGACCCATGGCCCAACAGCATGGTGGTGGAACACCCC GAGTTCCTCAAGGCAGGGAAGGAGCCTGGCCTGCAGATCTGGCGTGTGGAGAAGTTCGATCTGGTGCCC GTGCCCACCAACCTTTATGGAGACTTCTTCACGGGCGACGCCTACGTCATCCTGAAGACAGTGCAGCTG AGGAACGGAAATCTGCAGTATGACCTCCACTACTGGCTGGGCAATGAGTGCAGCCAGGATGAGAGCGGG GCGGCCGCCATCTTTACCGTGCAGCTGGATGACTACCTGAACGGCCGGGCCGTGCAGCACCGTGAGGTC CAGGGCTTCGAGTCGGCCACCTTCCTAGGCTACTTCAAGTCTGGCCTGAAGTACAAGAAAGGAGGTGTG GCATCAGGATTCAAGCACGTGGTACCCAACGAGGTGGTGGTGCAGAGACTCTTCCAGGTCAAAGGGCGG CGTGTGGTCCGTGCCACCGAGGTACCTGTGTCCTGGGAGAGCTTCAACAATGGCGACTGCTTCATCCTG GACCTGGGCAACAACATCCACCAGTGGTGTGGTTCCAACAGCAATCGGTATGAAAGACTGAAGGCCACA CAGGTGTCCAAGGGCATCCGGGACAACGAGCGGAGTGGCCGGGCCCGAGTGCACGTGTCTGAGGAGGGC ACTGAGCCCGAGGCGATGCTCCAGGTGCTGGGCCCCAAGCCGGCTCTGCCTGCAGGTACCGAGGACACC GCCAAGGAGGATGCGGCCAACCGCAAGCTGGCCAAGCTCTACAAGGTCTCCAATGGTGCAGGGACCATG TCCGTCTCCCTCGTGGCTGATGAGAACCCCTTCGCCCAGGGGGCCCTGAAGTCAGAGGACTGCTTCATC CTGGACCACGGCAAAGATGGGAAAATCTTTGTCTGGAAAGGCAAGCAGGCAAACACGGAGGAGAGGAAG GCTGCCCTCAAAACAGCCTCTGACTTCATCACCAAGATGGACTACCCCAAGCAGACTCAGGTCTCGGTC CTTCCTGAGGGCGGTGAGACCCCACTGTTCAAGCAGTTCTTCAAGAACTGGCGGGACCCAGACCAGACA GATGGCCTGGGCTTGTCCTACCTTTCCAGCCATATCGCCAACGTGGAGCGGGTGCCCTTCGACGCCGCC ACCCTGCACACCTCCACTGCCATGGCCGCCCAGCACGGCATGGATGACGATGGCACAGGCCAGAAACAG ATCTGGAGAATCGAAGGTTCCAACAAGGTGCCCGTGGACCCTGCCACATATGGACAGTTCTATGGAGGC GACAGCTACATCATTCTGTACAACTACCGCCATGGTGGCCGCCAGGGGCAGATAATCTATAACTGGCAG GGTGCCCAGTCTACCCAGGATGAGGTCGCTGCATCTGCCATCCTGACTGCTCAGCTGGATGAGGAGCTG GGAGGTACCCCTGTCCAGAGCCGTGTGGTCCAAGGCAAGGAGCCCGCCCACCTCATGAGCCTGTTTGGT GGGAAGCCCATGATCATCTACAAGGGCGGCACCTCCCGCGAGGGCGGGCAGACAGCCCCTGCCAGCACC CGCCTCTTCCAGGTCCGCGCCAACAGCGCTGGAGCCACCCGGGCTGTTGAGGTATTGCCTAAGGCTGGT GCACTGAACTCCAACGATGCCTTTGTTCTGAAAACCCCCTCAGCCGCCTACCTGTGGGTGGGTACAGGA GCCAGCGAGGCAGAGAAGACGGGGGCCCAGGAGCTGCTCAGGGTGCTGCGGGCCCAACCTGTGCAGGTG GCAGAAGGCAGCGAGCCAGATGGCTTCTGGGAGGCCCTGGGCGGGAAGGCTGCCTACCGCACATCCCCA CGGCTGAAGGACAAGAAGATGGATGCCCATCCTCCTCGCCTCTTTGCCTGCTCCAACAAGATTGGACGT TTTGTGATCGAAGAGGTTCCTGGTGAGCTCATGCAGGAAGACCTGGCAACGGATGACGTCATGCTTCTG GACACCTGGGACCAGGTCTTTGTCTGGGTTGGAAAGGATTCTCAAGAAGAAGAAAAGACAGAAGCCTTG ACTTCTGCTAAGCGGTACATCGAGACGGACCCAGCCAATCGGGATCGGCGGACGCCCATCACCGTGGTG AAGCAAGGCTTTGAGCCTCCCTCCTTTGTGGGCTGGTTCCTTGGCTGGGATGATGATTACTGGTCTGTG GACCCCTTGGACAGGGCCATGGCTGAGCTGGCTGCCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001258029
Insert Size	2247 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001258029.1
RefSeq Size	2579 bp
RefSeq ORF	2247 bp
Locus ID	2934
UniProt ID	P06396
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton
MW	82.5 kDa
Gene Summary	The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (9) uses an alternate first exon compared to that of variant 1. The resulting isoform (d) has a shorter and distinct N-terminus compared to isoform a.

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