Beta Arrestin 2 (ARRB2) (NM_001257331) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARB2; ARR2; BARR2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332528 representing NM_001257331. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGGGGAGAAACCCGGGACCAGGGTCTTCAAGAAGTCGAGCCCTAACTGCAAGCTCACCGTGTACTTG GGCAAGCGGGACTTCGTAGATCACCTGGACAAAGTGGACCCTGTAGTGTTTGTGACCCTCACCTGCGCC TTCCGCTATGGCCGTGAAGACCTGGATGTGCTGGGCTTGTCCTTCCGCAAAGACCTGTTCATCGCCACC TACCAGGCCTTCCCCCCGGTGCCCAACCCACCCCGGCCCCCCACCCGCCTGCAGGACCGGCTGCTGAGG AAGCTGGGCCAGCATGCCCACCCCTTCTTCTTCACCATACCCCAGAATCTTCCATGCTCCGTCACACTG CAGCCAGGCCCAGAGGATACAGGAAAGGCCTGCGGCGTAGACTTTGAGATTCGAGCCTTCTGTGCTAAA TCACTAGAAGAGAAAAGCCACAAAAGGAACTCTGTGCGGCTGGTGATCCGAAAGGTGCAGTTCGCCCCG GAGAAACCCGGCCCCCAGCCTTCAGCCGAAACCACACGCCACTTCCTCATGTCTGACCGGTCCCTGCAC CTCGAGGCTTCCCTGGACAAGGAGCTGTACTACCATGGGGAGCCCCTCAATGTAAATGTCCACGTCACC AACAACTCCACCAAGACCGTCAAGAAGATCAAAGTCTCTGTGAGACAGTACGCCGACATCTGCCTCTTC AGCACCGCCCAGTACAAGTGTCCTGTGGCTCAACTCGAACAAGATGACCAGGTATCTCCCAGCTCCACA TTCTGTAAGGTGTACACCATAACCCCACTGCTCAGCGACAACCGGGAGAAGCGGGGTCTCGCCCTGGAT GGGAAACTCAAGCACGAGGACACCAACCTGGCTTCCAGCACCATCGTGAAGGAGGGTGCCAACAAGGAG GTGCTGGGAATCCTGGTGTCCTACAGGGTCAAGGTGAAGCTGGTGGTGTCTCGAGGCGGGGATGTCTCT GTGGAGCTGCCTTTTGTTCTTATGCACCCCAAGCCCCACGACCACATCCCCCTCCCCAGACCCCAGTCA GCACCCACCCCCACACCCCCTCTTCCCGTCCCCCCAGCCGCTCCGGAGACAGATGTCCCTGTGGACACC AACCTCATTGAATTTGATACCAACTATGCCACAGATGATGACATTGTGTTTGAGGACTTTGCCCGGCTT CGGCTGAAGGGGATGAAGGATGACGACTATGATGATCAACTCTGCTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001257331
Insert Size	1221 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001257331.1
RefSeq Size	1927 bp
RefSeq ORF	1221 bp
Locus ID	409
UniProt ID	P32121
Protein Families	Druggable Genome
Protein Pathways	Chemokine signaling pathway, Endocytosis, MAPK signaling pathway, Olfactory transduction
MW	45.6 kDa
Gene Summary	Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012] Transcript Variant: This variant (6) lacks an alternate in-frame exon, uses an alternate in-frame splice junction at the 3' end of an exon, and uses an alternate in-frame splice junction at the 5' end of another exon compared to variant 3. The resulting isoform (6) lacks two alternate internal segments and contains another alternate internal segment compared to isoform 3.

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