DDX19B (NM_001257175) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DBP5; DDX19; RNAh
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332515 representing NM_001257175. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCTTAGCCAAGTAGAACCTGCAAACAAATACCCCCAGTGTCTATGTCTCTCCCCAACGTATGAGCTC GCCCTCCAAACAGGAAAAGTGATTGAACAAATGGGCAAATTTTACCCTGAACTGAAGCTAGCTTATGCT GTTCGAGGCAATAAATTGGAAAGAGGCCAGAAGATCAGTGAGCAGATTGTCATTGGCACCCCTGGGACT GTGCTGGACTGGTGCTCCAAGCTCAAGTTCATTGATCCCAAGAAAATCAAGGTGTTTGTTCTGGATGAG GCTGATGTCATGATAGCCACTCAGGGCCACCAAGATCAGAGCATCCGCATCCAGAGGATGCTGCCCAGG AACTGCCAGATGCTGCTTTTCTCCGCCACCTTTGAAGACTCTGTGTGGAAGTTTGCCCAGAAAGTGGTC CCAGACCCAAACGTTATCAAACTGAAGCGTGAGGAAGAGACCCTGGACACCATCAAGCAGTACTATGTC CTGTGCAGCAGCAGAGACGAGAAGTTCCAGGCCTTGTGTAACCTCTACGGGGCCATCACCATTGCTCAA GCCATGATCTTCTGCCATACTCGCAAAACAGCTAGTTGGCTGGCAGCAGAGCTCTCAAAAGAAGGCCAC CAGGTGGCTCTGCTGAGTGGGGAGATGATGGTGGAACAGAGGGCTGCAGTGATTGAGCGCTTCCGAGAG GGCAAAGAGAAGGTTTTGGTGACCACCAACGTGTGTGCCCGCGGCATTGATGTTGAACAAGTGTCTGTC GTCATCAACTTTGATCTTCCCGTGGACAAGGACGGGAATCCTGACAATGAGACCTACCTGCACCGGATC GGGCGCACGGGCCGCTTTGGCAAGAGGGGCCTGGCAGTGAACATGGTGGACAGCAAGCACAGCATGAAC ATCCTGAACAGAATCCAGGAGCATTTTAATAAGAAGATAGAAAGATTGGACACAGATGATTTGGACGAG ATTGAGAAAATAGCCAACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001257175
Insert Size	987 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001257175.1
RefSeq Size	1633 bp
RefSeq ORF	987 bp
Locus ID	11269
MW	37.4 kDa
Gene Summary	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which exhibits RNA-dependent ATPase and ATP-dependent RNA-unwinding activities. This protein is recruited to the cytoplasmic fibrils of the nuclear pore complex, where it participates in the export of mRNA from the nucleus. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (7) lacks 3 exons in the 5' region compared to variant 1. This results in translation initiation from a downstream start codon and a protein (isoform 5) which has a distinct N-terminus and is shorter compared to isoform 1.

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