DDX11 (NM_001257145) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CHL1; CHLR1; KRG2; WABS
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332507 representing NM_001257145. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCAGAGCTGTACCGGGTTTTGGAGGCTGGCAAGATTGGGATATTTGAGAGTCCAACTGGCACTGGG AAGTCCTTAAGTCTTATTTGTGGGGCCCTCTCTTGGCTCCGTGACTTTGAACAGAAGAAGCGTGAAGAA GAGGCACGACTCCTTGAAACTGGAACTGGCCCCTTACATGATGAGAAAGATGAATCCCTGTGTCTGTCT TCTTCCTGCGAAGGGGCTGCAGGCACCCCGAGGCCTGCTGGAGAACCGGCCTGGGTTACTCAGTTTGTG CAGAAGAAAGAAGAGAGGGACCTGGTGGACCGACTAAAGGCGGAGCAGGCCAGGAGGAAGCAGCGAGAA GAACGCCTGCAGCAGCTGCAGCACAGGGTGCAGCTCAAGTATGCAGCCAAGCGCCTGAGGCAGGAAGAA GAAGAAAGAGAGAATCTCCTCCGCCTCAGCAGGGAGATGCTAGAGACAGGCCCGGAGGCTGAGCGGCTG GAGCAGCTGGAGTCTGGGGAGGAGGAGCTGGTCCTCGCCGAATACGAGAGTGATGAGGAGAAAAAGGTG GCGAGCAGAGTGGATGAGGATGAGGATGACCTGGAGGAAGAACACATAACTAAGATTTATTACTGTAGT CGGACACACTCCCAGCTGGCCCAGTTTGTGCATGAGGTGAAGAAGAGCCCCTTTGGCAAGGATGTTCGG CTGGTCTCCCTTGGCTCCCGGCAGAACCTTTGTGTAAATGAAGACGTGAAAAGCCTAGGTTCTGTGCAG CTTATCAACGACCGCTGTGTGGACATGCAGAGAAGCAGGCACGAGAAGAAGAAAGGAGCTGAGGAGGAG AAGCCAAAGAGGAGGAGGCAGGAGAAGCAGGCAGCCTGCCCCTTCTACAACCACGAGCAGATGGGCCTT CTCCGGGATGAGGCCCTGGCAGAGGTGAAGGACATGGAGCAGCTGCTGGCCCTTGGGAAGGAGGCCCGG GCCTGTCCCTATTACGGGAGCCGCCTTGCCATCCCTGCAGCCCAGCTGGTGGTGCTGCCCTATCAGATG CTGCTGCATGCGGCCACTCGGCAGGCCGCGGGCATCCGGCTGCAGGACCAGGTGGTGATCATCGACGAG GCGCACAACCTGATCGACACCATCACGGGCATGCACAGCGTGGAGGTCAGCGGCTCCCAGCTCTGCCAG GCCCATTCCCAGCTGCTGCAGTACGTGGAGCGATACGGGAAGCGTTTGAAGGCCAAGAACCTGATGTAC CTGAAGCAGATCCTGTATTTGCTGGAGAAATTCGTGGCTGTGCTAGGGGGGAACATTAAGCAAAATCCC AATACACAGAGTCTGTCACAGACAGGGACGGAGCTGAAGACCATCAACGACTTTCTCTTCCAGAGCCAG ATCGACAACATCAACCTGTTCAAGGTGCAGCGATACTGTGAGAAGAGCATGATCAGCAGAAAGCTCTTT GGATTCACTGAACGGTACGGAGCAGTGTTCTCATCCCGGGAGCAGCCCAAACTGGCTGGGTTTCAGCAA TTCCTGCAGAGCCTGCAGCCCAGGACGACTGAAGCTCTTGCAGCCCCTGCAGACGAGAGTCAGGCCAGC ACCCTGCGACCAGCTTCTCCACTGATGCACATCCAAGGCTTCCTGGCAGCTCTCACTACGGCCAACCAG GACGGCAGGGTCATCCTGAGCCGCCAAGGCAGCCTCAGTCAGAGCACCCTGAAGTTTTTGCTCCTGAAT CCAGCTGTGCACTTTGCCCAAGTGGTGAAGGAATGCCGGGCAGTGGTCATTGCGGGGGGTACCATGCAG CCGGTGTCTGACTTCCGGCAGCAGCTGCTGGCCTGTGCCGGGGTGGAAGCTGAGCGCGTGGTGGAGTTT TCCTGTGGTCACGTGATCCCTCCAGACAACATCCTGCCCCTCGTCATCTGCAGCGGGATCTCCAACCAG CCGCTGGAATTCACGTTCCAGAAAAGAGAGCTGCCTCAGATGATGGACGAGGTGGGTCGCATTCTCTGT AACCTGTGCGGTGTGGTTCCTGGAGGGGTGGTCTGTTTCTTCCCCTCCTACGAGTACCTGCGCCAGGTC CATGCCCACTGGGAGAAGGGTGGCCTGCTGGGCCGTCTGGCTGCCAGGAAGAAGATATTCCAGGAACCT AAGAGCGCACACCAGGTGGAGCAGGTGCTGCTGGCATATTCCAGGTGCATCCAGGCCTGTGGCCAGGAG AGAGGCCAGGTGACAGGGGCCCTGCTCCTCTCTGTGGTTGGAGGAAAGATGAGTGAAGGGATCAACTTC TCTGACAACCTAGGCCGGTGTGTGGTGATGGTGGGCATGCCCTTCCCCAACATCAGGTCTGCAGAGCTG CAGGAGAAGATGGCCTACTTGGATCAAACCCTCCCCAGAGCCCCCGGCCAGGCACCCCCAGGGAAGGCT CTGGTGGAGAACCTGTGCATGAAGGCCGTCAACCAGTCCATAGGCAGGGCCATCAGGCACCAGAAGGAT TTTGCCAGCGTAGTGCTCCTGGACCAGCGATATGCCCGGCCCCCTGTCCTGGCCAAGCTGCCGGCCTGG ATCCGAGCCCGTGTGGAGGTCAAAGCTACCTTTGGCCCCGCCATTGCTGCTGTGCAGAAGTTTCACCGG GAGAAGTCGGCCTCTTCCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001257145
Insert Size	2643 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001257145.1
RefSeq Size	3737 bp
RefSeq ORF	2643 bp
Locus ID	1663
UniProt ID	Q96FC9
Protein Families	Stem cell - Pluripotency
MW	98.7 kDa
Gene Summary	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. This gene is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (5) differs in the 5' UTR and coding region, and uses a downstream AUG compared to variant 3. The resulting protein (isoform 4) has shorter N- and C-termini compared to isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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