DDX11 (NM_001257144) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CHL1; CHLR1; KRG2; WABS
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332506 representing NM_001257144. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCTAATGAAACACAGAAGGTTGGTGCCATCCATTTTCCTTTTCCCTTCACACCCTATTCCATCCAG GAAGACTTCATGGCAGAGCTGTACCGGGTTTTGGAGGCTGGCAAGATTGGGATATTTGAGAGTCCAACT GGCACTGGGAAGTCCTTAAGTCTTATTTGTGGGGCCCTCTCTTGGCTCCGTGACTTTGAACAGAAGAAG CGTGAAGAAGAGGCACGACTCCTTGAAACTGGAACTGGCCCCTTACATGATGAGAAAGATGAATCCCTG TGTCTGTCTTCTTCCTGCGAAGGGGCTGCAGGCACCCCGAGGCCTGCTGGAGAACCGGCCTGGGTTACT CAGTTTGTGCAGAAGAAAGAAGAGAGGGACCTGGTGGACCGACTAAAGGCGGAGCAGGCCAGGAGGAAG CAGCGAGAAGAACGCCTGCAGCAGCTGCAGCACAGGGTGCAGCTCAAGTATGCAGCCAAGCGCCTGAGG CAGGAAGAAGAAGAAAGAGAGAATCTCCTCCGCCTCAGCAGGGAGATGCTAGAGACAGGCCCGGAGGCT GAGCGGCTGGAGCAGCTGGAGTCTGGGGAGGAGGAGCTGGTCCTCGCCGAATACGAGAGTGATGAGGAG AAAAAGGTGGCGAGCAGAGTGGATGAGGATGAGGATGACCTGGAGGAAGAACACATAACTAAGATTTAT TACTGTAGTCGGACACACTCCCAGCTGGCCCAGTTTGTGCATGAGGTGAAGAAGAGCCCCTTTGGCAAG GATGTTCGGCTGGTCTCCCTTGGCTCCCGGCAGAACCTTTGTGTAAATGAAGACGTGAAAAGCCTAGGT TCTGTGCAGCTTATCAACGACCGCTGTGTGGACATGCAGAGAAGCAGGCACGAGAAGAAGAAAGGAGCT GAGGAGGAGAAGCCAAAGAGGAGGAGGCAGGAGAAGCAGGCAGCCTGCCCCTTCTACAACCACGAGCAG ATGGGCCTTCTCCGGGATGAGGCCCTGGCAGAGGTGAAGGACATGGAGCAGCTGCTGGCCCTTGGGAAG GAGGCCCGGGCCTGTCCCTATTACGGGAGCCGCCTTGCCATCCCTGCAGCCCAGCTGGTGGTGCTGCCC TATCAGATGCTGCTGCATGCGGCCACTCGGCAGGCCGCGGGCATCCGGCTGCAGGACCAGGTGGTGATC ATCGACGAGGCGCACAACCTGATCGACACCATCACGGGCATGCACAGCGTGGAGGTCAGCGGCTCCCAG CTCTGCCAGGCCCATTCCCAGCTGCTGCAGTACGTGGAGCGATACGGGAAGCGTTTGAAGGCCAAGAAC CTGATGTACCTGAAGCAGATCCTGTATTTGCTGGAGAAATTCGTGGCTGTGCTAGGGGGGAACATTAAG CAAAATCCCAATACACAGAGTCTGTCACAGACAGGGACGGAGCTGAAGACCATCAACGACTTTCTCTTC CAGAGCCAGATCGACAACATCAACCTGTTCAAGGTGCAGCGATACTGTGAGAAGAGCATGATCAGCAGA AAGCTCTTTGGATTCACTGAACGGTACGGAGCAGTGTTCTCATCCCGGGAGCAGCCCAAACTGGCTGGG TTTCAGCAATTCCTGCAGAGCCTGCAGCCCAGGACGACTGAAGCTCTTGCAGCCCCTGCAGACGAGAGT CAGGCCAGCACCCTGCGACCAGCTTCTCCACTGATGCACATCCAAGGCTTCCTGGCAGCTCTCACTACG GCCAACCAGGACGGCAGGGTCATCCTGAGCCGCCAAGGCAGCCTCAGTCAGAGCACCCTGAAGTTTTTG CTCCTGAATCCAGCTGTGCACTTTGCCCAAGTGGTGAAGGAATGCCGGGCAGTGGTCATTGCGGGGGGT ACCATGCAGCCGGTGTCTGACTTCCGGCAGCAGCTGCTGGCCTGTGCCGGGGTGGAAGCTGAGCGCGTG GTGGAGTTTTCCTGTGGTCACGTGATCCCTCCAGACAACATCCTGCCCCTCGTCATCTGCAGCGGGATC TCCAACCAGCCGCTGGAATTCACGTTCCAGAAAAGAGAGCTGCCTCAGATGATGGACGAGGTGGGTCGC ATTCTCTGTAACCTGTGCGGTGTGGTTCCTGGAGGGGTGGTCTGTTTCTTCCCCTCCTACGAGTACCTG CGCCAGGTCCATGCCCACTGGGAGAAGGGTGGCCTGCTGGGCCGTCTGGCTGCCAGGAAGAAGATATTC CAGGAACCTAAGAGCGCACACCAGGTGGAGCAGGTGCTGCTGGCATATTCCAGGTGCATCCAGGCCTGT GGCCAGGAGAGAGGCCAGGTGACAGGGGCCCTGCTCCTCTCTGTGGTTGGAGGAAAGATGAGTGAAGGG ATCAACTTCTCTGACAACCTAGGCCGGTGTGTGGTGATGGTGGGCATGCCCTTCCCCAACATCAGGTCT GCAGAGCTGCAGGAGAAGATGGCCTACTTGGATCAAACCCTCAGCCCCCGGCCAGGCACCCCCAGGGAA GGCTCTGGTGGAGAACCTGTGCATGAAGGCCGTCAACCAGTCCATAGGCAGGGCCATCAGGCACCAGAA GGATTTTGCCAGCGTAGTGCTCCTGGACCAGCGATATGCCCGGCCCCCTGTCCTGGCCAAGCTGCCGGC CTGGATCCGAGCCCGTGTGGAGGTCAAAGCTACCTTTGGCCCCGCCATTGCTGCTGTGCAGAAGTTTCA CCGGGAGAAGTCGGCCTCTTCCTGATGGGCAACCACACCACTGCCTGGCGCCGTGCCCTTCCTTTGTCC TGCCCGCTGGAGACAGTGTTTGTCGTGGGCGTGGTCTGCGGGGATCCTGTTACAAAGGTGAAACCCAGG AGGAGAGTGTGGAGTCCAGAGTGCTGCCAGGACCCAGGCACAGGCGTTAGCTCCCGTAGGAGAAAATGG GGGAATCCTGAATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001257144
Insert Size	2913 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001257144.1
RefSeq Size	3823 bp
RefSeq ORF	2913 bp
Locus ID	1663
UniProt ID	Q96FC9
Protein Families	Stem cell - Pluripotency
MW	108.3 kDa
Gene Summary	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. This gene is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 3. Variants 3 and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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