GUCY1A1 (NM_001256449) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3; GUCA3; GUCSA3; GUCY1A3; MYMY6
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332387 representing NM_001256449. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTTCTGCACGAAGCTCAAGGATCTCAAGATCACAGGAGAGTGTCCTTTCTCCTTACTGGCACCAGGT CAAGTTCCTAACGAGTCTTCAGAGGAGGCAGCAGGAAGCTCAGAGAGCTGCAAAGCAACCGTGCCCATC TGTCAAGACATTCCTGAGAAGAACATACAAGAAAGTCTTCCTCAAAGAAAAACCAGTCGGAGCCGAGTC TATCTTCACACTTTGGCAGAGAGTATTTGCAAACTGATTTTCCCAGAGTTTGAACGGCTGAATGTTGCA CTTCAGAGAACATTGGCAAAGCACAAAATAAAAGAAAGCAGGAAATCTTTGGAAAGAGAAGACTTTGAA AAAACAATTGCAGAGCAAGCAGTTGCAGCAGGAGTTCCAGTGGAGGTTATCAAAGAATCTCTTGGTGAA GAGGTTTTTAAAATATGTTACGAGGAAGATGAAAACATCCTTGGGGTGGTTGGAGGCACCCTTAAAGAT TTTTTAAACAGCTTCAGTACCCTTCTGAAACAGAGCAGCCATTGCCAAGAAGCAGGAAAAAGGGGCAGG CTTGAGGACGCCTCCATTCTATGCCTGGATAAGGAGGATGATTTTCTACATGTTTACTACTTCTTCCCT AAGAGAACCACCTCCCTGATTCTTCCCGGCATCATAAAGGCAGCTGCTCACGTATTATATGAAACGGAA GTGGAAGTGTCGTTAATGCCTCCCTGCTTCCATAATGATTGCAGCGAGTTTGTGAATCAGCCCTACTTG TTGTACTCCGTTCACATGAAAAGCACCAAGCCATCCCTGTCCCCCAGCAAACCCCAGTCCTCGCTGGTG ATTCCCACATCGCTATTCTGCAAGACATTTCCATTCCATTTCATGTTTGACAAAGATATGACAATTCTG CAATTTGGCAATGGCATCAGAAGGCTGATGAACAGGAGAGACTTTCAAGGAAAGCCTAATTTTGAAGAA TACTTTGAAATTCTGACTCCAAAAATCAACCAGACGTTTAGCGGGATCATGACTATGTTGAATATGCAG TTTGTTGTACGAGTGAGGAGATGGGACAACTCTGTGAAAAAATCTTCAAGGGTTATGGACCTCAAAGGC CAAATGATCTACATTGTTGAATCCAGTGCAATCTTGTTTTTGGGGTCACCCTGTGTGGACAGATTAGAA GATTTTACAGGACGAGGGCTCTACCTCTCAGACATCCCAATTCACAATGCACTGAGGGATGTGGTCTTA ATAGGGGAACAAGCCCGAGCTCAAGATGGCCTGAAGAAGAGGCTGGGGAAGCTGAAGGCTACCCTTGAG CAAGCCCACCAAGCCCTGGAGGAGGAGAAGAAAAAGACAGTAGACCTTCTGTGCTCCATATTTCCCTGT GAGGTTGCTCAGCAGCTGTGGCAAGGGCAAGTTGTGCAAGCCAAGAAGTTCAGTAATGTCACCATGCTC TTCTCAGACATCGTTGGGTTCACTGCCATCTGCTCCCAGTGCTCACCGCTGCAGGTCATCACCATGCTC AATGCACTGTACACTCGCTTCGACCAGCAGTGTGGAGAGCTGGATGTCTACAAGGTGGAGACCATTGGC GATGCCTATTGTGTAGCTGGGGGATTACACAAAGAGAGTGATACTCATGCTGTTCAGATAGCGCTGATG GCCCTGAAGATGATGGAGCTCTCTGATGAAGTTATGTCTCCCCATGGAGAACCTATCAAGATGCGAATT GGACTGCACTCTGGATCAGTTTTTGCTGGCGTCGTTGGAGTTAAAATGCCCCGTTACTGTCTTTTTGGA AACAATGTCACTCTGGCTAACAAATTTGAGTCCTGCAGTGTACCACGAAAAATCAATGTCAGCCCAACA ACTTACAGATTACTCAAAGACTGTCCTGGTTTCGTGTTTACCCCTCGATCAAGGGAGGAACTTCCACCA AACTTCCCTAGTGAAATCCCCGGAATCTGCCATTTTCTGGATGCTTACCAACAAGGAACAAACTCAAAA CCATGCTTCCAAAAGAAAGATGTGGAAGATGGCAATGCCAATTTTTTAGGCAAAGCATCAGGAATAGAT TAG
Restriction Sites	SgfI-MluI
ACCN	NM_001256449
Insert Size	2073 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256449.1
RefSeq Size	9291 bp
RefSeq ORF	2073 bp
Locus ID	2982
UniProt ID	Q02108
Protein Families	Druggable Genome
Protein Pathways	Gap junction, Long-term depression, Purine metabolism, Vascular smooth muscle contraction
MW	77.5 kDa
Gene Summary	Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (8) differs in the 5' UTR, compared to variant 1, and encodes isoform A. Variants 1, 2, 3, 4, and 8 all encode the same isoform (A). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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