PDLIM5 (NM_001256427) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ENH; ENH1; L9; LIM
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332382 representing NM_001256427. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAGCAACTACAGTGTGTCACTGGTTGGCCCAGCTCCTTGGGGTTTCCGGCTGCAGGGCGGTAAGGAT TTCAACATGCCTCTGACAATCTCTAGTCTAAAAGATGGCGGCAAGGCAGCCCAGGCAAATGTAAGAATA GGCGATGTGGTTCTCAGCATTGATGGAATAAATGCACAAGGAATGACTCATCTTGAAGCCCAGAATAAG ATTAAGGGTTGTACAGGCTCTTTGAATATGACTCTGCAAAGAGCATCTGCTGCACCCAAGCCTGAGCCG GTTCCTGTTCAAAAGCCCACAGTCACCAGCGTGTGTTCCGAGACTTCTCAGGAGCTAGCAGAGGGACAG AGAAGAGGATCCCAGGGTGACAGTAAACAGCAAAATGGGAAAATCCCACCTAAACGCCCACCAAGAAAA CACATTGTGGAGCGCTATACAGAGTTTTATCATGTACCCACTCACAGTGATGCCAGCAAGAAGAGACTG ATTGAGGATACTGAAGACTGGCGTCCAAGGACTGGAACAACTCAGTCTCGCTCTTTCCGAATCCTTGCC CAGATCACTGGGACTGAACATTTGAAAGAATCTGAAGCCGATAATACAAAGAAGGCAAATAACTCTCAG GAGCCTTCTCCGCAGTTGGCTTCCTCGGTAGCTTCCACACGGAGCATGCCCGAGAGCCTGGACAGCCCA ACCTCTGGCAGACCAGGGGTTACCAGCCTCACAACTGCAGCTGCCTTCAAGCCTGTAGGATCCACTGGC GTCATCAAGTCACCAAGCTGGCAACGGCCAAACCAAGGAGTACCTTCCACTGGAAGAATCTCAAACAGC GCTACTTACTCAGGATCAGTGGCACCAGCCAACTCAGCTTTGGGACAAACCCAGCCAAGTGACCAGGAC ACTTTAGTGCAAAGAGCTGAGCACATTCCAGCAGGGAAACGAACTCCGATGTGCGCCCATTGTAACCAG GTCATCAGAGGACCATTCTTAGTGGCACTGGGGAAATCTTGGCACCCAGAAGAATTCAACTGCGCTCAC TGCAAAAATACAATGGCCTACATTGGATTTGTAGAGGAGAAAGGAGCCCTGTATTGTGAGCTGTGCTAT GAGAAATTCTTTGCCCCTGAATGTGGTCGATGCCAAAGGAAGATCCTTGGAGAAGTCATCAGTGCGTTG AAACAAACTTGGCATGTTTCCTGTTTTGTGTGTGTAGCCTGTGGAAAGCCCATTCGGAACAATGTTTTT CACTTGGAGGATGGTGAACCCTACTGTGAGACTGATTATTATGCCCTCTTTGGTACTATATGCCATGGA TGTGAATTTCCCATAGAAGCTGGTGACATGTTCCTGGAAGCTCTGGGCTACACCTGGCATGACACTTGC TTTGTATGCTCAGTGTGTTGTGAAAGTTTGGAAGGTCAGACCTTTTTCTCCAAGAAGGACAAGCCCCTG TAA
Restriction Sites	SgfI-MluI
ACCN	NM_001256427
Insert Size	1452 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256427.1
RefSeq Size	3834 bp
RefSeq ORF	1452 bp
Locus ID	10611
Protein Families	Druggable Genome
MW	52.7 kDa
Gene Summary	This gene encodes a member of a family of proteins that possess a 100-amino acid PDZ domain at the N terminus and one to three LIM domains at the C-terminus. This family member functions as a scaffold protein that tethers protein kinases to the Z-disk in striated muscles. It is thought to function in cardiomyocyte expansion and in restraining postsynaptic growth of excitatory synapses. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (7) uses an alternate in-frame splice site in the 5' coding region, contains an additional in-frame exon in the central coding region, and uses alternate 3' exon structure and thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (g) has a distinct C-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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