FBXL11 (KDM2A) (NM_001256405) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CXXC8; FBL7; FBL11; FBXL11; JHDM1A; LILINA
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332373 representing NM_001256405. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTGCTCTGGGAGATTCCAGAATATTCAAGTAAATCCGGATTTTCCCAGAGGCAGAATATCTAACTCC TTCAGGAGAACTTCCAGTACAGAAAACAAAACAAAGACGCTGGGGAAACTTCACCAAGAGCCTCGGCAG CTCCAGAGCGACGGGAAACGAAAGATTTTGCTGGAGGAGCTTGCCAACAGCGATCCCAAGTTAGCCCTC ACTGGAGTTCCTATAGTACAGTGGCCAAAAAGGGATAAGCTTAAATTCCCCACTCGGCCAAAGGTGCGG GTTCCTACCATCCCCATTACGAAGCCTCACACTATGAAACCAGCTCCACGGTTAACACCTGTGAGGCCA GCTGCTGCCTCCCCGATTGTGTCAGGAGCCAGACGGAGACGAGTGCGATGTCGAAAATGCAAAGCCTGT GTGCAAGGAGAGTGTGGTGTTTGCCACTACTGCAGAGACATGAAGAAGTTTGGGGGGCCTGGACGCATG AAGCAGTCCTGTGTCCTCCGACAGTGCTTGGCACCCAGACTGCCTCACTCAGTCACATGTTCCCTCTGT GGAGAGGTGGATCAGAATGAAGAGACACAAGACTTTGAGAAGAAACTCATGGAATGCTGTATCTGCAAT GAGATTGTTCATCCTGGCTGCCTCCAGATGGACGGAGAGGGGTTGCTTAACGAAGAATTGCCAAATTGC TGGGAATGTCCAAAGTGCTACCAGGAGGACAGCTCGGAGAAAGCCCAGAAGCGGAAAATGGAAGAGAGT GACGAAGAAGCTGTGCAAGCCAAAGTCCTGCGGCCCCTGCGGAGCTGCGATGAGCCTCTCACGCCCCCG CCTCATTCACCCACTTCCATGCTGCAGCTCATCCATGACCCGGTTTCCCCCCGGGGTATGGTGACTCGG TCATCCCCTGGGGCTGGCCCCAGCGACCACCACAGTGCCAGCCGCGATGAGCGCTTCAAACGGCGGCAG TTGCTGCGGCTGCAGGCCACAGAGCGCACCATGGTACGGGAAAAGGAGAACAATCCCAGCGGCAAAAAG GAGCTGTCTGAAGTTGAGAAAGCCAAGATCCGGGGATCGTACCTCACTGTCACGCTACAGAGGCCCACC AAAGAGCTCCACGGGACATCCATTGTGCCCAAGCTGCAGGCCATCACGGCCTCCTCTGCCAACCTTCGC CATTCCCCCCGTGTGCTAGTGCAGCACTGCCCAGCCCGAACCCCCCAGCGTGGGGATGAGGAGGGGCTG GGGGGAGAGGAGGAGGAAGAGGAGGAGGAGGAGGAGGAAGATGACAGTGCAGAGGAGGGGGGTGCAGCC AGGCTGAATGGCCGGGGCAGTTGGGCTCAGGATGGAGACGAAAGCTGGATGCAGCGGGAGGTCTGGATG TCTGTCTTCCGCTACCTCAGCCGCAGAGAACTTTGTGAATGTATGCGAGTGTGCAAGACGTGGTATAAA TGGTGCTGCGACAAGAGACTTTGGACAAAAATTGACTTGAGTAGGTGTAAGGCCATTGTGCCCCAGGCC CTCAGTGGCATCATCAAGAGGCAGCCAGTCAGCCTTGACCTCAGTTGGACCAACATCTCTAAAAAGCAA CTGACATGGCTCGTCAATAGGCTGCCAGGACTGAAAGACCTCCTCCTAGCAGGCTGCTCCTGGTCTGCA GTCTCTGCCCTCAGCACCTCCAGCTGCCCCCTTCTCAGGACCCTTGATCTTCGGTGGGCAGTAGGAATC AAGGACCCTCAAATTCGGGACTTGCTTACTCCACCGGCTGATAAACCAGGTCAGGACAATCGCAGCAAG CTCCGGAACATGACCGACTTCCGGCTGGCAGGCCTTGACATCACAGATGCCACGCTTCGCCTCATAATT CGCCACATGCCCCTCCTGTCTCGACTCGACCTCAGTCACTGCAGCCACCTTACAGATCAGTCCTCCAAT CTACTCACTGCTGTCGGGTCTTCCACTCGCTACTCTCTCACAGAGCTCAATATGGCAGGTTGCAATAAA TTGACAGACCAGACCCTGATCTACCTACGGCGCATTGCCAACGTCACCTTGATCGACCTTCGAGGATGC AAGCAGATCACTCGAAAAGCCTGCGAGCACTTCATCTCAGACTTGTCCATCAACAGCCTCTACTGCCTG TCTGACGAGAAGCTGATACAGAAGATCAGCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001256405
Insert Size	2172 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256405.1
RefSeq Size	5379 bp
RefSeq ORF	2172 bp
Locus ID	22992
UniProt ID	Q9Y2K7
Protein Families	Druggable Genome, Transcription Factors
MW	81.7 kDa
Gene Summary	This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains at least six highly degenerated leucine-rich repeats. This family member plays a role in epigenetic silencing. It nucleates at CpG islands and specifically demethylates both mono- and di-methylated lysine-36 of histone H3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (3) lacks several 5' exons but contains an alternate 5' exon, uses an internal downstream promoter, and differs in its 5' UTR and 5' coding region, compared to variant 1. The encoded isoform (b, also known as SF-KDM2A or short-form KDM2A) has a distinct and shorter N-terminus, compared to isoform a.

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