CHD1L (NM_001256337) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ALC1; CHDL
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332359 representing NM_001256337. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTCAGCATTGCAGAAGAAATACTACAAGGCCATTTTGATGAAAGACCTAGATGCATTTGAAAATGAG ACGGCAAAGAAAGTTAAACTACAGAACATTTTGTCCCAGCTTCGAAAGTGTGTGGATCACCCATATTTG TTTGATGGTGTGGAGCCGGAGCCTTTTGAAGTTGGAGACCACCTGACTGAGGCTAGTGGGAAGCTTCAC CTGCTGGATAAGCTACTAGCATTCCTGTATTCTGGGGGCCATCGGGTTTTACTTTTCTCCCAAATGACC CAGATGTTGGATATTCTCCAAGACTATATGGATTACAGAGGCTACAGCTATGAGCGTGTGGATGGTTCT GTGAGAGGAGAAGAGAGACACTTGGCCATTAAGAACTTTGGACAGCAGCCCATTTTCGTTTTTCTCCTG AGTACTAGGGCAGGTGGAGTTGGCATGAACTTAACAGCAGCAGATACTGTGATTTTTGTTGACAGTGAC TTTAATCCTCAGAATGACTTGCAAGCAGCTGCCAGGGCTCATCGCATTGGCCAAAACAAGTCTGTTAAA GTTATTCGGCTGATTGGTCGAGACACTGTGGAAGAAATAGTCTATAGGAAAGCAGCCTCCAAACTGCAG CTCACCAACATGATCATAGAAGGAGGCCATTTTACTCTGGGAGCCCAGAAACCCGCTGCCGATGCTGAC CTCCAGTTGAGTGAGATACTCAAATTTGGTTTGGATAAACTGCTGGCCTCTGAGGGGAGCACCATGGAT GAAATAGACCTGGAGTCCATCCTGGGAGAAACAAAAGATGGCCAGTGGGTCTCTGATGCCTTGCCTGCA GCAGAAGGAGGGAGCAGAGATCAAGAGGAAGGAAAAAATCATATGTACTTATTTGAAGGTAAAGATTAT TCTAAAGAGCCCAGTAAGGAAGACAGAAAATCATTTGAACAACTGGTAAACCTTCAGAAAACCCTTTTG GAGAAAGCTAGTCAAGAGGGCCGATCACTCCGAAATAAAGGCAGTGTTCTCATCCCAGGCCTTGTGGAG GGATCTACCAAAAGGAAGCGGGTTCTGAGTCCAGAAGAGCTGGAGGACAGACAGAAGAAAAGACAAGAA GCAGCTGCCAAGAGAAGGAGACTCATAGAGGAGAAGAAGAGGCAAAAGGAAGAGGCTGAACATAAGAAA AAGATGGCCTGGTGGGAATCCAACAATTACCAGTCCTTCTGCCTGCCCTCTGAGGAGAGCGAGCCAGAG GACCTTGAGAATGGGGAAGAGAGCTCTGCTGAGCTGGATTACCAAGACCCAGATGCTACTTCCCTCAAG TACGTTAGTGGTGATGTCACCCACCCTCAGGCTGGGGCCGAGGATGCTCTCATTGTGCACTGCGTAGAT GACTCTGGCCACTGGGGCAGAGGTGGTTTATTTACAGCTCTGGAAAAGCGATCCGCTGAGCCAAGAAAA ATATATGAGCTGGCTGGGAAAATGAAAGACCTGAGTTTGGGAGGTGTCCTTTTATTTCCTGTTGATGAT AAAGAATCAAGAAACAAAGGGCAAGATTTGTTGGCCTTGATTGTGGCTCAGCATCGTGATCGTTCCAAT GTCCTGTCTGGCATTAAGATGGCAGCCCTAGAAGAGGGCCTGAAGAAGATATTTTTAGCAGCAAAAAAG AAGAAAGCAAGTGTTCATCTTCCACGTATTGGACATGCCACGAAAGGTTTTAACTGGTATGGTACTGAG CGACTTATTCGGAAACATCTGGCTGCAAGAGGCATCCCAACTTACATATATTATTTTCCTAGAAGCAAG TCTGCTGTCCTTCATTCACAGTCTTCATCTTCCTCCTCAAGACAGCTGGTGCCTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001256337
Insert Size	1851 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256337.1
RefSeq Size	2506 bp
RefSeq ORF	1851 bp
Locus ID	9557
UniProt ID	Q86WJ1
MW	69.2 kDa
Gene Summary	This gene encodes a DNA helicase protein involved in DNA repair. The protein converts ATP to add poly(ADP-ribose) as it regulates chromatin relaxation following DNA damage. Overexpression of this gene has been linked to several types of cancers. [provided by RefSeq, Feb 2017] Transcript Variant: This variant (4) lacks several exons in the 5' coding region which results in translation from a downstream start codon, compared to variant 1. The resulting protein (isoform 4) is shorter when it is compared to isoform 1. Variants 4 and 12-22 all encode the same isoform (4). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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