SGLT1 (SLC5A1) (NM_001256314) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	D22S675; NAGT; SGLT1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332345 representing NM_001256314. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCAGAGTACCTGAGGAAGCGGTTTGGAGGCCAGCGGATCCAGGTCTACCTTTCCCTTCTGTCCCTG CTGCTCTACATTTTCACCAAGATCTCGGCAGACATCTTCTCGGGGGCCATATTCATCAATCTGGCCTTA GGCCTGAATCTGTATTTAGCCATCTTTCTCTTATTGGCAATCACTGCCCTTTACACAATTACAGGGGGC CTGGCGGCGGTGATTTACACGGACACCTTGCAGACGGTGATCATGCTGGTGGGGTCTTTAATCCTGACT GGGTTTGCTTTTCACGAAGTGGGAGGCTATGACGCCTTCATGGAAAAGTACATGAAAGCCATTCCAACC ATAGTGTCTGATGGCAACACCACCTTTCAGGAAAAATGCTACACTCCAAGGGCCGACTCCTTCCACATC TTCCGAGATCCCCTCACGGGAGACCTCCCATGGCCTGGGTTCATCTTTGGGATGTCCATCCTTACCTTG TGGTACTGGTGCACAGATCAGGTCATTGTGCAGCGCTGCCTCTCAGCCAAGAATATGTCTCACGTGAAG GGTGGCTGCATCCTGTGTGGGTATCTAAAGCTGATGCCCATGTTCATCATGGTGATGCCAGGAATGATC AGCCGCATTCTGTACACAGAAAAAATTGCCTGTGTCGTCCCTTCAGAATGTGAGAAATATTGCGGTACC AAGGTTGGCTGTACCAACATCGCCTATCCAACCTTAGTGGTGGAGCTCATGCCCAATGGACTGCGAGGC CTGATGCTATCAGTCATGCTGGCCTCCCTCATGAGCTCCCTGACCTCCATCTTCAACAGCGCCAGCACC CTCTTCACCATGGACATCTACGCCAAGGTCCGCAAGAGAGCATCTGAGAAAGAGCTCATGATTGCCGGA AGGTTGTTTATCCTGGTGCTGATTGGCATCAGCATCGCCTGGGTGCCCATTGTGCAGTCAGCACAAAGT GGGCAACTCTTCGATTACATCCAGTCCATCACCAGTTACTTGGGACCACCCATTGCGGCTGTCTTCCTG CTTGCTATTTTCTGGAAGAGAGTCAATGAGCCAGGAGCCTTTTGGGGACTGATCCTAGGACTTCTGATT GGGATTTCACGTATGATTACTGAGTTTGCTTATGGAACCGGGAGCTGCATGGAGCCCAGCAACTGTCCC ACGATTATCTGTGGGGTGCACTACTTGTACTTTGCCATTATCCTCTTCGCCATTTCTTTCATCACCATC GTGGTCATCTCCCTCCTCACCAAACCCATTCCGGATGTGCATCTCTACCGTCTGTGTTGGAGCCTGCGC AACAGCAAAGAGGAGCGTATTGACCTGGATGCGGAAGAGGAGAACATCCAAGAAGGCCCTAAGGAGACC ATTGAAATAGAAACACAAGTTCCTGAGAAGAAAAAAGGAATCTTCAGGAGAGCCTATGACCTATTTTGT GGGCTAGAGCAGCACGGTGCACCCAAGATGACTGAGGAAGAGGAGAAAGCCATGAAGATGAAGATGACG GACACCTCTGAGAAGCCTTTGTGGAGGACAGTGTTGAACGTCAATGGCATCATCCTGGTGACCGTGGCT GTCTTTTGCCATGCATATTTTGCCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001256314
Insert Size	1614 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256314.1
RefSeq Size	4779 bp
RefSeq ORF	1614 bp
Locus ID	6523
UniProt ID	P13866
Protein Families	Druggable Genome, Transmembrane
MW	60.1 kDa
Gene Summary	This gene encodes a member of the sodium-dependent glucose transporter (SGLT) family. The encoded integral membrane protein is the primary mediator of dietary glucose and galactose uptake from the intestinal lumen. Mutations in this gene have been associated with glucose-galactose malabsorption. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (2) lacks several 5' exons compared to variant 1. This variant represents translation initiation at a downstream AUG compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a weak Kozak sequence and a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG. The encoded protein (isoform 2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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