Dystrobrevin beta (DTNB) (NM_001256303) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332338 representing NM_001256303. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGATTGAGGAAAGTGGGAACAAGCGGAAGACCATGGCAGAGAAGAGGCAGCTGTTCATAGAAATGCGT GCTCAGAATTTTGATGTCATACGACTATCAACTTACAGAACAGCCTGCAAATTACGATTTGTACAAAAA CGATGCAACCTTCATCTTGTTGATATCTGGAACATGATTGAAGCCTTCCGAGACAATGGCCTTAATACA CTGGACCATACCACCGAGATCAGTGTGTCCCGCCTCGAAACTGTCATCTCCTCCATCTACTATCAGTTG AACAAGCGCCTTCCTTCTACTCACCAAATTAGTGTGGAACAATCTATCAGCCTCCTCCTCAACTTTATG ATTGCTGCATATGACAGTGAGGGCCGAGGCAAGTTGACGGTATTTTCAGTTAAAGCTATGTTAGCAACC ATGTGTGGTGGAAAAATGCTGGACAAATTGAGATATGTTTTCTCCCAGATGTCAGATTCCAATGGCTTA ATGATATTTAGCAAGTTTGACCAGTTTCTGAAGGAAGTTCTGAAGCTCCCAACAGCTGTCTTTGAAGGG CCATCTTTTGGTTACACAGAGCACTCAGTCCGCACCTGTTTTCCACAGCAGAGAAAGATAATGCTAAAT ATGTTTTTAGACACAATGATGGCTGACCCTCCTCCCCAGTGCCTTGTCTGGCTACCTCTCATGCACAGG CTTGCCCATGTTGAGAATGTCTTCCATCCCGTGGAGTGCTCCTACTGCCGATGTGAGAGTATGATGGGT TTCCGGTACCGATGCCAGCAGTGCCACAACTATCAGCTCTGCCAGAATTGCTTTTGGCGTGGCCATGCC GGCGGCCCTCACAGCAACCAGCACCAGATGAAGGAGCATTCCTCTTGGAAATCTCCTGCAAAGAAGCTG AGCCATGCAATTAGTAAATCTTTGGGGTGTGTACCCACGAGAGAACCCCCGCATCCTGTTTTTCCTGAG CAACCAGAGAAACCACTTGACCTTGCACATATAGTTCCTCCTCGCCCTCTGACTAATATGAATGACACC ATGGTTAGCCACATGTCCTCTGGAGTGCCCACTCCCACCAAGAGGTTACAGTATAGCCAGGATATACCC AGTCACTTGGCCGATGAGCATGCGCTGATAGCCTCCTATGTGGCCCGTCTGCAGCACTGTGCACGTGTT CTGGACAGTCCTAGCCGACTGGATGAGGAACACCGTCTTATAGCTCGCTATGCTGCCCGGCTGGCTGCA GAAGCAGGAAACGTGACTCGTCCTCCCACTGACTTGAGCTTTAACTTTGATGCCAACAAACAACAAAGA CAGCTTATTGCAGAACTGGAAAACAAAAACAGAGAGATCCTGCAGGAGATTCAGCGTCTCCGCCTGGAA CACGAGCAGGCCTCCCAGCCCACCCCTGAGAAGGCACAGCAGAACCCCACGCTGCTGGCAGAGCTGCGG CTGCTGAGGCAAAGGAAGGATGAACTGGAGCAGAGGATGTCGGCCCTGCAGGAGAGCAGGCGGGAGCTG ATGGTCCAGCTGGAAGAGCTGATGAAGTTGCTGAAGGCTCAGGCCACAGGGTCACCACATACATCGCCC ACCCATGGAGGCGGCCGGCCAATGCCCATGCCAGTGCGCTCCACGTCTGCCGGCTCCACCCCCACCCAC TGTCCGCAGGACTCGCTGAGCGGAGTCGGGGGAGACGTGCAGGAGGCCTTCGCACAAGGTACGAGGAGA AACCTCCGCAATGACCTGCTGGTGGCAGCTGACTCCATCACCAACACCATGTCATCCCTGGTGAAGGAG CTCCATTCAGCAGAGGAAGGTGCAGAGGAAGAAGAAGAGAAGATGCAGAATGGGAAAGACAGAGGTTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001256303
Insert Size	1863 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256303.1
RefSeq Size	2486 bp
RefSeq ORF	1863 bp
Locus ID	1838
UniProt ID	O60941
MW	70.5 kDa
Gene Summary	This gene encodes dystrobrevin beta, a component of the dystrophin-associated protein complex (DPC). The DPC consists of dystrophin and several integral and peripheral membrane proteins, including dystroglycans, sarcoglycans, syntrophins and dystrobrevin alpha and beta. The DPC localizes to the sarcolemma and its disruption is associated with various forms of muscular dystrophy. Dystrobrevin beta is thought to interact with syntrophin and the DP71 short form of dystrophin. [provided by RefSeq, Mar 2016] Transcript Variant: This variant (6) lacks an in-frame exon in the 3' coding region, compared to variant 1. The resulting isoform (6) lacks an internal segment, compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.