KIF22 (NM_001256270) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	A-328A3.2; KID; KNSL4; OBP; OBP-1; OBP-2; SEMDJL2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332331 representing NM_001256270. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGACAGCTGCTCTCTAGAGATTGCTAACTGGAGGAACCACCAGGAGACTCTCAAATACCAGTTTGAT GCCTTCTATGGGGAGAGGAGTACTCAGCAGGACATCTATGCAGGTTCAGTGCAGCCCATCCTAAGGCAC TTGCTGGAAGGGCAGAATGCCAGTGTGCTTGCCTATGGACCCACAGGAGCTGGGAAGACGCACACAATG CTGGGCAGCCCAGAGCAACCTGGGGTGATCCCGCGGGCTCTCATGGACCTCCTGCAGCTCACAAGGGAG GAGGGTGCCGAGGGCCGGCCATGGGCCCTTTCTGTCACCATGTCTTACCTAGAGATCTACCAGGAGAAG GTATTAGACCTCCTGGACCCTGCTTCGGGAGACCTGGTAATCCGAGAAGACTGCCGGGGGAATATCCTG ATTCCGGGTCTCTCCCAGAAGCCCATCAGTAGCTTTGCTGATTTTGAGCGGCACTTCCTGCCAGCCAGT CGAAATCGGACTGTAGGAGCCACCCGGCTCAACCAGCGCTCCTCCCGCAGTCATGCTGTGCTCCTGGTC AAGGTGGACCAGCGGGAACGTTTGGCCCCATTTCGCCAGCGAGAGGGAAAACTCTACCTGATTGACTTG GCTGGGTCAGAGGACAACCGGCGCACAGGCAACAAGGGCCTTCGGCTAAAAGAGAGTGGAGCCATCAAC ACCTCCCTGTTTGTCCTGGGCAAAGTGGTAGATGCGCTGAATCAGGGCCTCCCTCGTGTACCTTATCGG GACAGCAAGCTCACTCGCCTATTGCAGGACTCTCTGGGTGGCTCAGCCCACAGTATCCTTATTGCCAAC ATTGCCCCTGAGAGACGCTTCTACCTAGACACAGTCTCCGCACTCAACTTTGCTGCCAGGTCCAAGGAG GTGATCAATCGGCCTTTTACCAATGAGAGCCTGCAGCCTCATGCCTTGGGACCTGTTAAGCTGTCTCAG AAAGAATTGCTTGGTCCACCAGAGGCAAAGAGAGCCCGAGGCCCTGAGGAAGAGGAGATCGGGAGCCCT GAGCCCATGGCAGCTCCAGCCTCTGCCTCCCAGAAACTCAGCCCCCTACAGAAGCTAAGCAGCATGGAC CCGGCCATGCTGGAGCGCCTCCTCAGCTTGGACCGTCTGCTTGCCTCCCAGGGGAGCCAGGGGGCCCCT CTGTTGAGTACCCCAAAGCGAGAGCGGATGGTGCTAATGAAGACAGTGGAAGAGAAGGACCTAGAGATT GAGAGGCTTAAGACGAAGCAAAAAGAACTGGAGGCCAAGATGTTGGCCCAGAAGGCTGAGGAAAAGGAG AACCATTGTCCCACAATGCTCCGGCCCCTTTCACATCGCACAGTCACAGGGGCAAAGCCCCTGAAAAAG GCTGTGGTGATGCCCCTACAGCTAATTCAGGAGCAGGCAGCATCCCCAAATGCCGAGATCCACATCCTG AAGAATAAAGGCCGGAAGAGAAAGCTGGAGTCCCTGGATGCCCTAGAGCCTGAGGAGAAGGCTGAGGAC TGCTGGGAGCTACAGATCAGCCCGGAGCTACTGGCTCATGGGCGCCAAAAAATACTGGATCTGCTGAAC GAAGGCTCAGCCCGAGATCTCCGCAGTCTTCAGCGCATTGGCCCGAAGAAGGCCCAGCTAATCGTGGGC TGGCGGGAGCTCCACGGCCCCTTCAGCCAGGTGGAGGACCTGGAACGCGTGGAGGGCATAACGGGGAAA CAGATGGAGTCCTTCCTGAAGGCAAACATCCTGGGTCTCGCCGCCGGCCAGCGCTGTGGCGCCTCCTGA
Restriction Sites	SgfI-RsrII
ACCN	NM_001256270
Insert Size	1794 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256270.1
RefSeq Size	2296 bp
RefSeq ORF	1794 bp
Locus ID	3835
UniProt ID	Q14807
Protein Families	Druggable Genome
MW	66.3 kDa
Gene Summary	The protein encoded by this gene is a member of the kinesin-like protein family. The family members are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. The C-terminal half of this protein has been shown to bind DNA. Studies with the Xenopus homolog suggests its essential role in metaphase chromosome alignment and maintenance. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (3) has an alternate 5' exon, which results in a downstream AUG start codon, compared to variant 1. The resulting isoform (2) is shorter at the N-terminus than isoform 1. Variants 2 and 3 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.