ADGRE1 (NM_001256253) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EMR1; TM7LN3
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332325 representing NM_001256253. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCGTGGCTTCAACCTGCTCCTCTTCTGGGGATGTTGTGTTATGCACAGCTGGGAAGGGCACATAAGA CCCACACGGAAACCAAACACAAAGGGTAATAACTGTAGAGACAGTACCTTGTGCCCAGCTTATGCCACC TGCACCAATACAGTGGACAGTTACTATTGCGCTTGCAAACAAGGCTTCCTGTCCAGCAATGGGCAAAAT CACTTCAAGGATCCAGGAGTGCGATGCAAAGATATTGATGAATGTTCTCAAAGCCCCCAGCCCTGTGGT CCTAACTCATCCTGCAAAAACCTGTCAGGGAGGTACAAGTGCAGCTGTTTAGATGGTTTCTCTTCTCCC ACTGGAAATGACTGGGTCCCAGGAAAGCCGGGCAATTTCTCCTGTACTGATATCAATGAGTGCCTCACC AGCAGCGTCTGCCCTGAGCATTCTGACTGTGTCAACTCCATGGGAAGCTACAGTTGCAGCTGTCAAGTT GGATTCATCTCTAGAAACTCCACCTGTGAAGACGTGGATGAATGTGCAGATCCAAGAGCTTGCCCAGAG CATGCAACTTGTAATAACACTGTTGGAAACTACTCTTGTTTCTGCAACCCAGGATTTGAATCCAGCAGT GGCCACTTGAGTTTCCAGGGTCTCAAAGCATCGTGTGAAGATATTGATGAATGCACTGAAATGTGCCCC ATCAATTCAACATGCACCAACACTCCTGGGAGCTACTTTTGCACCTGCCACCCTGGCTTTGCACCAAGC AATGGACAGTTGAATTTCACAGACCAAGGAGTGGAATGTAGAGATATTGATGAGTGCCGCCAAGATCCA TCAACCTGTGGTCCTAATTCTATCTGCACCAATGCCCTGGGCTCCTACAGCTGTGGCTGCATTGCAGGC TTTCATCCCAATCCAGAAGGCTCCCAGAAAGATGGCAACTTCAGCTGCCAAAGGGTTCTCTTCAAATGT AAGGAAGATGTGATACCCGATAATAAGCAGATCCAGCAATGCCAAGAGGGAACCGCAGTGAAACCTGCA TATGTCTCCTTTTGTGCACAAATAAATAACATCTTCAGCGTTCTGGACAAAGTGTGTGAAAATAAAACG ACCGTAGTTTCTCTGAAGAATACAACTGAGAGCTTTGTCCCTGTGCTTAAACAAATATCCACGTGGACT AAATTCACCAAGGAAGAGACGTCCTCCCTGGCCACAGTCTTCCTGGAGAGTGTGGAAAGCATGACACTG GCATCTTTTTGGAAACCCTCAGCAAATATCACTCCGGCTGTTCGGACGGAATACTTAGACATTGAGAGC AAAGTTATCAACAAAGAATGCAGTGAAGAGAATGTGACGTTGGACTTGGTAGCCAAGGGGGATAAGATG AAGATCGGGTGTTCCACAATTGAGGAATCTGAATCCACAGAGACCACTGGTGTGGCTTTTGTCTCCTTT GTGGGCATGGAATCGGTTTTAAATGAGCGCTTCTTCAAAGACCACCAGGCTCCCTTGACCACCTCTGAG ATCAAGCTGAAGATGAATTCTCGAGTCGTTGGGGGCATAATGACTGGAGAGAAGAAAGACGGCTTCTCA GATCCAATCATCTACACTCTGGAGAACATTCAGCCAAAGCAGAAGTTTGAGAGGCCCATCTGTGTTTCC TGGAGCACTGATGTGAAGGGTGGAAGATGGACATCCTTTGGCTGTGTGATCCTGGAAGCTTCTGAGACA TATACCATCTGCAGCTGTAATCAGATGGCAAATCTTGCCGTTATCATGGCGTCTGGGGAGCTCACGATG GGCTGCGCCATCATCGCGGGCTTCCTGCACTACCTTTTCCTTGCCTGCTTCTTCTGGATGCTGGTGGAG GCTGTGATACTGTTCTTGATGGTCAGAAACCTGAAGGTGGTGAATTACTTCAGCTCTCGCAACATCAAG ATGCTGCACATCTGTGCCTTTGGTTATGGGCTGCCGATGCTGGTGGTGGTGATCTCTGCCAGTGTGCAG CCACAGGGCTATGGAATGCATAATCGCTGCTGGCTGAATACAGAGACAGGGTTCATCTGGAGTTTCTTG GGGCCAGTTTGCACAGTTATAGTGATCAACTCCCTTCTCCTGACCTGGACCTTGTGGATCCTGAGGCAG AGGCTTTCCAGTGTTAATGCCGAAGTCTCAACGCTAAAAGACACCAGGTTACTGACCTTCAAGGCCTTT GCCCAGCTCTTCATCCTGGGCTGCTCCTGGGTGCTGGGCATTTTTCAGATTGGACCTGTGGCAGGTGTC ATGGCTTACCTGTTCACCATCATCAACAGCCTGCAGGGGGCCTTCATCTTCCTCATCCACTGTCTGCTC AACGGCCAGGTACGAGAAGAATACAAGAGGTGGATCACTGGGAAGACGAAGCCCAGCTCCCAGTCCCAG ACCTCAAGGATCTTGCTGTCCTCCATGCCATCCGCTTCCAAGACGGGTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001256253
Insert Size	2466 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256253.1
RefSeq Size	2966 bp
RefSeq ORF	2466 bp
Locus ID	2015
UniProt ID	Q14246
Protein Families	Druggable Genome, Transmembrane
MW	90.4 kDa
Gene Summary	This gene encodes a protein that has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (3) lacks an in-frame exon in the coding region, compared to variant 1. The resulting isoform (3) lacks an internal segment, compared to isoform 1.

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