ARHGAP22 (NM_001256026) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RhoGAP2; RhoGap22
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332289 representing NM_001256026. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCCTTCTGGCCCATCAGGTGTCTGAAGAGGAGCAGGCGTATGCCCAGGGGTGGTGCCGGGGAGCGG GAGAAGGTGCCGGCCAACCCCGAGGCGCTCCTGCTCATGGCCAGCTCCCAGCGTGACATGGAGGACTGG GTGCAGGCCATCCGCCGAGTCATCTGGGCCCCGCTGGGCGGAGGGATCTTTGGGCAGCGCCTAGAGGAA ACAGTCCACCACGAGCGGAAGTATGGCCCCCGCCTGGCGCCCCTGCTGGTGGAGCAGTGTGTGGACTTC ATCCGGGAGCGCGGGCTCACTGAGGAGGGGCTGTTCCGCATGCCAGGCCAGGCCAACCTGGTGAGGGAC CTGCAGGATTCCTTCGACTGTGGGGAGAAGCCACTGTTTGACAGCACAACAGACGTGCACACGGTGGCC TCCCTGCTGAAGCTGTACCTGCGGGAGCTCCCCGAGCCCGTGGTCCCCTTCGCCAGGTACGAGGACTTC CTCAGCTGCGCCCAGCTGCTCACCAAGGACGAGGGGGAGGGCACTCTGGAGTTGGCTAAACAAGTGAGC AACCTTCCTCAGGCAAATTACAACCTGCTCAGATACATCTGCAAGTTTCTGGATGAAGTTCAGGCATAC TCAAATGTCAACAAGATGAGTGTCCAGAATCTGGCAACCGTTTTTGGACCTAACATTCTGCGGCCACAG GTAGAGGACCCAGTAACCATCATGGAAGGCACTTCCCTCGTCCAGCACCTGATGACCGTCCTCATCCGC AAACACAGCCAGCTCTTCACGGCACCGGTCCCGGAAGGGCCCACCTCCCCGCGCGGGGGCCTGCAATGC GCAGTGGGGTGGGGCTCCGAGGAGGTCACCAGGGACAGCCAAGGAGAGCCCGGCGGCCCCGGCCTGCCC GCGCACAGGACCTCTTCCCTGGACGGGGCGGCCGTGGCGGTGCTCTCCAGAACAGCCCCCACGGGGCCG GGGAGCCGGTGCAGCCCTGGGAAGAAGGTGCAGACCCTGCCCAGTTGGAAGTCCTCCTTCCGGCAGCCG AGGTCCCTATCGGGAAGCCCGAAGGGGGGCGGCTCATCCCTGGAGGTGCCCATCATCTCCTCCGGCGGG AACTGGCTTATGAACGGGCTGTCCTCCCTGCGCGGACACCGCCGGGCCTCGTCGGGAGACCGGCTCAAG GACTCGGGCTCCGTGCAGAGACTCTCCACCTACGACAATGTGCCCGCGCCGGGCCTGGTCCCCGGCATA CCCAGCGTGGCCAGTATGGCGTGGTCCGGGGCCTCGTCCAGCGAGTCGTCGGTGGGGGGCTCACTCAGC AGCTGCACGGCCTGCCGCGCCAGCGACTCGTCTGCCCGCAGTTCCCTGCACACCGACTGGGCCCTGGAG CCCTCCCCGCTCCCCAGCAGCAGCGAGGACCCCAAGTCCCTGGACCTGGACCACAGCATGGACGAGGCG GGCGCGGGTGCCAGCAACAGCGAGCCCAGCGAGCCGGACAGCCCCACCCGGGAACACGCGCGCCGCTCC GAGGCCTTACAGGGGCTGGTCACTGAGCTCAGGGCCGAGCTGTGCCGCCAGCGGACTGAGTACGAGAGG AGTGTGAAAAGAATCGAAGAAGGGAGTGCTGACCTGAGAAAACGAATGTCCCGGTTAGAAGAAGAACTG GACCAGGAAAAGAAAAAATACATCATGCTGGAAATAAAGCTGCGGAACTCTGAACGGGCGCGGGAGGAT GCGGAGAGGAGGAACCAGCTGTTGCAGAGGGAAATGGAGGAGTTTTTTTCGACCCTAGGAAGCTTGACT GTTGGGGCAAAAGGTGCCAGGGCCCCAAAGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001256026
Insert Size	1827 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256026.1
RefSeq Size	2352 bp
RefSeq ORF	1827 bp
Locus ID	58504
UniProt ID	Q7Z5H3
MW	66.6 kDa
Gene Summary	This gene encodes a member of the GTPase activating protein family which activates a GTPase belonging to the RAS superfamily of small GTP-binding proteins. The encoded protein is insulin-responsive, is dependent on the kinase Akt and requires the Akt-dependent 14-3-3 binding protein which binds sequentially to two serine residues. The result of these interactions is regulation of cell motility. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] Transcript Variant: This variant (4) differs in the 5' UTR and lacks a portion of the 5' coding region, uses an alternate in-frame splice site in a 5' exon, and initiates translation at an alternate start codon compared to variant 1. The resulting protein (isoform 4) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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