GPR172A (SLC52A2) (NM_001253815) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BVVLS2; D15Ertd747e; GPCR41; GPR172A; hRFT3; PAR1; RFT3; RFVT2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332234 representing NM_001253815. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCAGCACCCACGCCCGCCCGTCCGGTGCTGACCCACCTGCTGGTGGCTCTCTTCGGCATGGGCTCC TGGGCTGCGGTCAATGGGATCTGGGTGGAGCTACCTGTGGTGGTCAAAGAGCTTCCAGAGGGTTGGAGC CTCCCCTCTTACGTCTCTGTGCTTGTGGCTCTGGGGAACCTGGGTCTGCTGGTGGTGACCCTCTGGAGG AGGCTGGCCCCAGGAAAGGACGAGCAGGTCCCCATCCGGGTGGTGCAGGTGCTGGGCATGGTGGGCACA GCCCTGCTGGCCTCTCTGTGGCACCATGTGGCCCCAGTGGCAGGACAGTTGCATTCTGTGGCCTTCTTA GCACTGGCCTTTGTGCTGGCACTGGCATGCTGTGCCTCGAATGTCACTTTCCTGCCCTTCTTGAGCCAC CTGCCACCTCGCTTCTTACGGTCATTCTTCCTGGGTCAAGGCCTGAGTGCCCTGCTGCCCTGCGTGCTG GCCCTAGTGCAGGGTGTGGGCCGCCTCGAGTGCCCGCCAGCCCCCATCAACGGCACCCCTGGCCCCCCG CTCGACTTCCTTGAGCGTTTTCCCGCCAGCACCTTCTTCTGGGCACTGACTGCCCTTCTGGTCGCTTCA GCTGCTGCCTTCCAGGGTCTTCTGCTGCTGTTGCCGCCACCACCATCTGTACCCACAGGGGAGTTAGGA TCAGGCCTCCAGGTGGGAGCCCCAGGAGCAGAGGAAGAGGTGGAAGAGTCCTCACCACTGCAAGAGCCA CCAAGCCAGGCAGCAGGCACCACCCCTGGTCCAGACCCTAAGGCCTATCAGCTTCTATCAGCCCGCAGT GCCTGCCTGCTGGGCCTGTTGGCCGCCACCAACGCGCTGACCAATGGCGTGCTGCCTGCCGTGCAGAGC TTTTCCTGCTTACCCTACGGGCGTCTGGCCTACCACCTGGCTGTGGTGCTGGGCAGTGCTGCCAATCCC CTGGCCTGCTTCCTGGCCATGGGTGTGCTGTGCAGGTCCTTGGCAGGGCTGGGCGGCCTCTCTCTGCTG GGCGTGTTCTGTGGGGGCTACCTGATGGCGCTGGCAGTCCTGAGCCCCTGCCCGCCCCTGGTGGGCACC TCGGCGGGGGTGGTCCTCGTGGTGCTGTCGTGGGTGCTGTGTCTTGGCGTGTTCTCCTACGTGAAGGTG GCAGCCAGCTCCCTGCTGCATGGCGGGGGCCGGCCGGCATTGCTGGCAGCCGGCGTGGCCATCCAGGTG GGCTCTCTGCTCGGCGCTGTTGCTATGTTCCCCCCGACCAGCATCTATCACGTGTTCCACAGCAGAAAG GACTGTGCAGACCCCTGTGACTCCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001253815
Insert Size	1338 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001253815.1
RefSeq Size	1806 bp
RefSeq ORF	1338 bp
Locus ID	79581
UniProt ID	Q9HAB3
Protein Families	Druggable Genome, GPCR, Transmembrane
MW	45.8 kDa
Gene Summary	This gene encodes a membrane protein which belongs to the riboflavin transporter family. In humans, riboflavin must be obtained by intestinal absorption because it cannot be synthesized by the body. The water-soluble vitamin riboflavin is processed to the coenzymes flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) which then act as intermediaries in many cellular metabolic reactions. Paralogous members of the riboflavin transporter gene family are located on chromosomes 17 and 20. Unlike other members of this family, this gene has higher expression in brain tissue than small intestine. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. Mutations in this gene have been associated with Brown-Vialetto-Van Laere syndrome 2 - an autosomal recessive progressive neurologic disorder characterized by deafness, bulbar dysfunction, and axial and limb hypotonia. [provided by RefSeq, Jul 2012] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3, 5, 6 and 7 encode the same isoform (1).

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