TIE1 (NM_001253357) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	JTK14; LMPHM11; TIE
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332213 representing NM_001253357. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTGGGCCGGGGCGGGGAGGGGCTCGGACGCCTGGGGCCCGCCCCTGCTGCTGGAGAAGGACGACCGT ATCGTGCGCACCCCGCCCGGGCCACCCCTGCGCCTGGCGCGCAACGGTTCGCACCAGGTCACGCTTCGC GGCTTCTCCAAGCCCTCGGACCTCGTGGGCGTCTTCTCCTGCGTGGGCGGTGCTGGGGCGCGGCGCACG CGCGTCATCTACGTGCACAACAGCCCTGGAGCCCACCTGCTTCCAGACAAGGTCACACACACTGTGAAC AAAGGTGACACCGCTGTACTTTCTGCACGTGTGCACAAGGAGAAGCAGACAGACGTGATCTGGAAGAGC AACGGATCCTACTTCTACACCCTGGACTGGCATGAAGCCCAGGATGGGCGGTTCCTGCTGCAGCTCCCA AATGTGCAGCCACCATCGAGCGGCATCTACAGTGCCACTTACCTGGAAGCCAGCCCCCTGGGCAGCGCC TTCTTTCGGCTCATCGTGCGGGGTTGTGGGGCTGGGCGCTGGGGGCCAGGCTGTACCAAGGAGTGCCCA GGTTGCCTACATGGAGGTGTCTGCCACGACCATGACGGCGAATGTGTATGCCCCCCTGGCTTCACTGGC ACCCGCTGTGAACAGGCCTGCAGAGAGGGCCGTTTTGGGCAGAGCTGCCAGGAGCAGTGCCCAGGCATA TCAGGCTGCCGGGGCCTCACCTTCTGCCTCCCAGACCCCTATGGCTGCTCTTGTGGATCTGGCTGGAGA GGAAGCCAGTGCCAAGAAGCTTGTGCCCCTGGTCATTTTGGGGCTGATTGCCGACTCCAGTGCCAGTGT CAGAATGGTGGCACTTGTGACCGGTTCAGTGGTTGTGTCTGCCCCTCTGGGTGGCATGGAGTGCACTGT GAGAAGTCAGACCGGATCCCCCAGATCCTCAACATGGCCTCAGAACTGGAGTTCAACTTAGAGACGATG CCCCGGATCAACTGTGCAGCTGCAGGGAACCCCTTCCCCGTGCGGGGCAGCATAGAGCTACGCAAGCCA GACGGCACTGTGCTCCTGTCCACCAAGGCCATTGTGGAGCCAGAGAAGACCACAGCTGAGTTCGAGGTG CCCCGCTTGGTTCTTGCGGACAGTGGGTTCTGGGAGTGCCGTGTGTCCACATCTGGCGGCCAAGACAGC CGGCGCTTCAAGGTCAATGTGAAAGTGCCCCCCGTGCCCCTGGCTGCACCTCGGCTCCTGACCAAGCAG AGCCGCCAGCTTGTGGTCTCCCCGCTGGTCTCGTTCTCTGGGGATGGACCCATCTCCACTGTCCGCCTG CACTACCGGCCCCAGGACAGTACCATGGACTGGTCGACCATTGTGGTGGACCCCAGTGAGAACGTGACG TTAATGAACCTGAGGCCAAAGACAGGATACAGTGTTCGTGTGCAGCTGAGCCGGCCAGGGGAAGGAGGA GAGGGGGCCTGGGGGCCTCCCACCCTCATGACCACAGACTGTCCTGAGCCTTTGTTGCAGCCGTGGTTG GAGGGCTGGCATGTGGAAGGCACTGACCGGCTGCGAGTGAGCTGGTCCTTGCCCTTGGTGCCCGGGCCA CTGGTGGGCGACGGTTTCCTGCTGCGCCTGTGGGACGGGACACGGGGGCAGGAGCGGCGGGAGAACGTC TCATCCCCCCAGGCCCGCACTGCCCTCCTGACGGGACTCACGCCTGGCACCCACTACCAGCTGGATGTG CAGCTCTACCACTGCACCCTCCTGGGCCCGGCCTCGCCCCCTGCACACGTGCTTCTGCCCCCCAGTGGG CCTCCAGCCCCCCGACACCTCCACGCCCAGGCCCTCTCAGACTCCGAGATCCAGCTGACATGGAAGCAC CCGGAGGCTCTGCCTGGGCCAATATCCAAGTACGTTGTGGAGGTGCAGGTGGCTGGGGGTGCAGGAGAC CCACTGTGGATAGACGTGGACAGGCCTGAGGAGACAAGCACCATCATCCGTGGCCTCAACGCCAGCACG CGCTACCTCTTCCGCATGCGGGCCAGCATTCAGGGGCTCGGGGACTGGAGCAACACAGTAGAAGAGTCC ACCCTGGGCAACGGGCTGCAGGCTGAGGGCCCAGTCCAAGAGAGCCGGGCAGCTGAAGAGGGCCTGGAT CAGCAGCTGATCCTGGCGGTGGTGGGCTCCGTGTCTGCCACCTGCCTCACCATCCTGGCTGCCCTTTTA ACCCTGGTGTGCATCCGCAGAAGCTGCCTGCATCGGAGACGCACCTTCACCTACCAGTCAGGCTCGGGC GAGGAGACCATCCTGCAGTTCAGCTCAGGGACCTTGACACTTACCCGGCGGCCAAAACTGCAGCCCGAG CCCCTGAGCTACCCAGTGCTAGAGTGGGAGGACATCACCTTTGAGGACCTCATCGGGGAGGGGAACTTC GGCCAGGTCATCCGGGCCATGATCAAGAAGGACGGGCTGAAGATGAACGCAGCCATCAAAATGCTGAAA GAGTATGCCTCTGAAAATGACCATCGTGACTTTGCGGGAGAACTGGAAGTTCTGTGCAAATTGGGGCAT CACCCCAACATCATCAACCTCCTGGGGGCCTGTAAGAACCGAGGTTACTTGTATATCGCTATTGAATAT GCCCCCTACGGGAACCTGCTAGATTTTCTGCGGAAAAGCCGGGTCCTAGAGACTGACCCAGCTTTTGCT CGAGAGCATGGGACAGCCTCTACCCTTAGCTCCCGGCAGCTGCTGCGTTTCGCCAGTGATGCGGCCAAT GGCATGCAGTACCTGAGTGAGAAGCAGTTCATCCACAGGGACCTGGCTGCCCGGAATGTGCTGGTCGGA GAGAACCTAGCCTCCAAGATTGCAGACTTCGGCCTTTCTCGGGGAGAGGAGGTTTATGTGAAGAAGACG ATGGGGCGTCTCCCTGTGCGCTGGATGGCCATTGAGTCCCTGAACTACAGTGTCTATACCACCAAGAGT GATGTCTGGTCCTTTGGAGTCCTTCTTTGGGAGATAGTGAGCCTTGGAGGTACACCCTACTGTGGCATG ACCTGTGCCGAGCTCTATGAAAAGCTGCCCCAGGGCTACCGCATGGAGCAGCCTCGAAACTGTGACGAT GAAGTGTACGAGCTGATGCGTCAGTGCTGGCGGGACCGTCCCTATGAGCGACCCCCCTTTGCCCAGATT GCGCTACAGCTAGGCCGCATGCTGGAAGCCAGGAAGGCCTATGTGAACATGTCGCTGTTTGAGAACTTC ACTTACGCGGGCATTGATGCCACAGCTGAGGAGGCCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001253357
Insert Size	3282 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001253357.1
RefSeq Size	3917 bp
RefSeq ORF	3282 bp
Locus ID	7075
Protein Families	Druggable Genome, Protein Kinase, Transmembrane
MW	120.2 kDa
Gene Summary	This gene encodes a member of the tyrosine protein kinase family. The encoded protein plays a critical role in angiogenesis and blood vessel stability by inhibiting angiopoietin 1 signaling through the endothelial receptor tyrosine kinase Tie2. Ectodomain cleavage of the encoded protein relieves inhibition of Tie2 and is mediated by multiple factors including vascular endothelial growth factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1.

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