ACSS1 (NM_001252677) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ACAS2L; ACECS1; AceCS2L
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332211 representing NM_001252677. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCGGCGCGCACCCTGGGCCGCGGCGTCGGGAGGCTGCTGGGCAGCCTGCGAGGGCTCTCGGGGCAG CCCGCGCGGCCGCCGTGCGGGGTGAGCGCGCCGCGCAGGGCGGCCTCGGGACCCTCGGGCAGCGCTCCC GCAGTTGCAGCAGCAGCAGCACAGCCAGGCTCGTATCCCGCGCTGAGTGCACAGGCAGCCCGGGAGCCG GCCGCCTTCTGGGGGCCTCTGGCGCGGGACACTCTCGTGTGGGACACCCCCTACCACACCGTCTGGGAC TGCGACTTCAGCACTGGCAAGATCGGCTGGTTCCTGGGAGGCCAGTTAAATGTCTCTGTCAACTGCTTG GACCAGCATGTTCGGAAGTCCCCCGAGAGCGTTGCTTTGATCTGGGAGCGCGATGAGCCTGGAACGGAA GTGAGGATCACCTACAGGGAACTACTGGAGACCACGTGCCGCCTGGCCAACACGCTGAAGAGGCATGGA GTCCACCGTGGGGACCGTGTTGCCATCTACATGCCCGTGTCCCCATTGGCTGTGGCAGCAATGCTGGCC TGTGCCAGGATCGGAGCTGTCCACACAGTCATCTTTGCTGGCTTCAGTGCAGAGTCCTTGGCTGGGAGG ATCAATGATGCCAAGTGCAAGGTGGTTATCACCTTCAACCAAGGACTCCGGGGTGGGCGCGTGGTGGAG CTGAAGAAAATAGTGGATGAGGCTGTGAAGCACTGCCCCACCGTGCAGCATGTCCTGGTGGCTCACAGG ACAGACAACAAGGTCCACATGGGGGATCTGGACGTCCCGCTGGAGCAGGAAATGGCCAAGGAGGACCCT GTTTGCGCCCCAGAGAGCATGGGCAGTGAGGACATGCTCTTCATGCTGTACACCTCAGGGAGCACCGGA ATGCCCAAGGGCATCGTCCATACCCAGGCAGGCTACCTGCTCTATGCCGCCCTGACTCACAAGCTTGTG TTTGACCACCAGCCAGGTGACATCTTTGGCTGTGTGGCCGACATCGGTTGGATTACAGGACACAGCTAC GTGGTGTATGGGCCTCTCTGCAATGGTGCCACCAGCGTCCTTTTTGAGAGCACCCCAGTTTATCCCAAT GCTGGTCGGTACTGGGAGACAGTAGAGAGGTTGAAGATCAATCAGTTCTATGGCGCCCCAACGGCTGTC CGGCTGTTGCTGAAATACGGTGATGCCTGGGTGAAGAAGTATGATCGCTCCTCCCTGCGGACCCTGGGG TCAGTGGGAGAGCCCATCAACTGTGAGGCCTGGGAGTGGCTTCACAGGGTGGTGGGGGACAGCAGGTGC ACGCTGGTGGACACCTGGTGGCAGACAGAAACAGGTGGCATCTGCATCGCACCACGGCCCTCGGAAGAA GGGGCGGAAATCCTCCCTGCCATGGCGATGAGGCCCTTCTTTGGCATCGTCCCCGTCCTCATGGATGAG AAGGGCAGCGTCGTGGAGGGCAGCAACGTCTCCGGGGCCCTGTGCATCTCCCAGGCCTGGCCGGGCATG GCCAGGACCATCTATGGCGACCACCAGCGATTTGTGGACGCCTACTTCAAGGCCTACCCAGGCTATTAC TTCACTGGAGACGGGGCTTACCGAACTGAGGGCGGCTATTACCAGATCACAGGGCGGATGGATGATGTC ATCAACATACTCCAAATAGTTGGGTTCTTCAGGGAAGCTATTAGAAACTCAGGTGACTTGTTAGAGCAC TAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252677
Insert Size	1728 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252677.1
RefSeq Size	2448 bp
RefSeq ORF	1728 bp
Locus ID	84532
UniProt ID	Q9NUB1
Protein Pathways	Glycolysis / Gluconeogenesis, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism
MW	62.7 kDa
Gene Summary	This gene encodes a mitochondrial acetyl-CoA synthetase enzyme. A similar protein in mice plays an important role in the tricarboxylic acid cycle by catalyzing the conversion of acetate to acetyl CoA. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (4) uses an alternate splice site in the 3' coding region, which results in a translational frameshift, compared to variant 1. The encoded isoform (4) is shorter and has a distinct C-terminus, compared to isoform 1.

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