EAAT2 (SLC1A2) (NM_001252652) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DEE41; EAAT2; EIEE41; GLT-1; HBGT
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332208 representing NM_001252652. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCCAAGCAGGTGGAAGTGCGAATGCACGACAGTCATCTTGGCTCAGAGGAACCCAAGCACCGGCAC CTGGGCCTGCGCCTGTGTGACAAGCTGGGGAAGAATCTGCTGCTCACCCTGACGGTGTTTGGTGTCATC CTGGGAGCAGTGTGTGGAGGGCTTCTTCGCTTGGCATCTCCCATCCACCCTGATGTGGTTATGTTAATA GCCTTCCCAGGGGATATACTCATGAGGATGCTAAAAATGCTCATTCTCCCTCTAATCATCTCCAGCTTA ATCACAGGGTTGTCAGGCCTGGATGCTAAGGCTAGTGGCCGCTTGGGCACGAGAGCCATGGTGTATTAC ATGTCCACGACCATCATTGCTGCAGTACTGGGGGTCATTCTGGTCTTGGCTATCCATCCAGGCAATCCC AAGCTCAAGAAGCAGCTGGGGCCTGGGAAGAAGAATGATGAAGTGTCCAGCCTGGATGCCTTCCTGGAC CTTATTCGAAATCTCTTCCCTGAAAACCTTGTCCAAGCCTGCTTTCAACAGATTCAAACAGTGACGAAG AAAGTCCTGGTTGCACCACCGCCGGACGAGGAGGCCAACGCAACCAGCGCTGTTGTCTCTCTGTTGAAC GAGACTGTGACTGAGGTGCCGGAGGAGACTAAGATGGTTATCAAGAAGGGCCTGGAGTTCAAGGATGGG ATGAACGTCTTAGGTCTGATAGGGTTTTTCATTGCTTTTGGCATCGCTATGGGGAAGATGGGAGATCAG GCCAAGCTGATGGTGGATTTCTTCAACATTTTGAATGAGATTGTAATGAAGTTAGTGATCATGATCATG TGGTACTCTCCCCTGGGTATCGCCTGCCTGATCTGTGGAAAGATCATTGCAATCAAGGACTTAGAAGTG GTTGCTAGGCAACTGGGGATGTACATGGTAACAGTGATCATAGGCCTCATCATCCACGGGGGCATCTTT CTCCCCTTGATTTACTTTGTAGTGACCAGGAAAAACCCCTTCTCCTTTTTTGCTGGCATTTTCCAAGCT TGGATCACTGCCCTGGGCACCGCTTCCAGTGCTGGAACTTTGCCTGTCACCTTTCGTTGCCTGGAAGAA AATCTGGGGATTGATAAGCGTGTGACTAGATTCGTCCTTCCTGTTGGAGCAACCATTAACATGGATGGT ACAGCCCTTTATGAAGCGGTAGCCGCCATCTTTATAGCCCAAATGAATGGTGTTGTCCTGGATGGAGGA CAGATTGTGACTGTAAGCCTCACAGCCACCCTGGCAAGCGTCGGCGCGGCCAGTATCCCCAGTGCCGGG CTGGTCACCATGCTCCTCATTCTGACAGCCGTGGGCCTGCCAACAGAGGACATCAGCCTGCTGGTGGCT GTGGACTGGCTGCTGGACAGGATGAGAACTTCAGTCAATGTTGTGGGTGACTCTTTTGGGGCTGGGATA GTCTATCACCTCTCCAAGTCTGAGCTGGATACCATTGACTCCCAGCATCGAGTGCATGAAGATATTGAA ATGACCAAGACTCAATCCATTTATGATGACATGAAGAACCACAGGGAAAGCAACTCTAATCAATGTGTC TATGCTGCACACAACTCTGTCATAGTAGATGAATGCAAGGTAACTCTGGCAGCCAATGGAAAGTCAGCC GACTGCAGTGTTGAGGAAGAACCTTGGAAACGTGAGAAATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252652
Insert Size	1698 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252652.1
RefSeq Size	11724 bp
RefSeq ORF	1698 bp
Locus ID	6506
UniProt ID	P43004
Protein Families	Transmembrane
Protein Pathways	Amyotrophic lateral sclerosis (ALS)
MW	61.2 kDa
Gene Summary	This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Improper regulation of this gene is thought to be associated with several neurological disorders. Alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jun 2017] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream, in-frame start codon, compared to variant 1. Variants 2 and 3 encode the same isoform (2), which is shorter than isoform 1.

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