THBS3 (NM_001252607) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	TSP3
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332199 representing NM_001252607. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGACGCAGGAACTTCGGGGGGCCCTGGCTCTTCTCCTCCTTTGCTTTTTCACATCTGCCAGTCAG GATCTGCAGGTAATTGACCTGCTGACTGTGGGCGAGTCTCGGCAGATGGTAGCTGTGGCAGAGAAGATC CGGACAGCCTTGCTCACTGCTGGGGACATCTACCTCTTATCCACCTTCCGCCTGCCCCCCAAGCAGGGT GGTGTCCTCTTTGGCCTCTATTCTCGCCAAGACAACACTCGATGGCTGGAGGCCTCTGTTGTAGGCAAG ATCAACAAAGTACTGGTGCGATACCAGCGGGAGGATGGCAAAGTCCACGCCGTGAACCTACAGCAAGCG GGCCTGGCTGATGGGCGCACACACACAGTTCTCCTGCGACTCCGAGGTCCCTCCAGACCCAGCCCTGCC CTACATCTCTACGTGGACTGCAAACTGGGTGACCAACATGCAGGCCTTCCAGCACTGGCCCCCATTCCT CCAGCGGAGGTCGATGGGCTGGAGATTAGGACTGGACAGAAGGCGTATTTGAGGATGCAGGGCTTTGTG GAATCTATGAAAATTATTCTGGGTGGGTCCATGGCCCGGGTAGGAGCCCTGAGTGAGTGTCCATTCCAA GGGGACGAGTCCATCCACAGTGCAGGGGAGCAGACCAAGGCGCTGGTCACCCAACTCACCCTCTTCAAC CAGATCCTGGTGGAGCTGCGGGATGATATACGAGACCAGGTGAAGGAAATGTCCCTGATCCGAAACACC ATTATGGAGTGTCAGGTGTGCGGCTTCCATGAGCAGCGTTCCCACTGCAGCCCCAATCCCTGCTTCCGA GGTGTGGACTGCATGGAAGTGTACGAGTACCCAGGCTACCGCTGTGGGCCCTGCCCCCCTGGCCTGCAG GGCAACGGCACCCACTGCAGTGACATCAATGAGTGTGCTCACGCTGACCCCTGTTTCCCGGGCTCCAGC TGCATCAACACCATGCCCGGCTTCCACTGTGAGGCCTGTCCTCGAGGGTACAAGGGCACACAGGTGTCT GGTGTGGGCATTGACTATGCCCGGGCCAGCAAACAGGTCTGCAATGACATCGATGAATGCAACGATGGC AACAATGGTGGCTGTGACCCAAACTCCATCTGCACCAACACTGTGGGCTCTTTCAAGTGTGGTCCCTGC CGCCTGGGTTTCCTGGGCAACCAGAGCCAGGGCTGCCTCCCAGCCCGGACCTGCCACAGCCCAGCCCAC AGCCCCTGCCACATCCATGCTCACTGTCTCTTTGAACGCAATGGTGCAGTGTCCTGCCAGTGTAACGTG GGCTGGGCTGGGAATGGGAACGTGTGTGGGACTGACACAGACATCGATGGCTACCCAGACCAAGCACTG CCCTGCATGGACAACAACAAACACTGCAAACAGGACAACTGCCTTTTGACACCCAACTCTGGGCAGGAA GATGCTGATAATGATGGTGTGGGGGACCAGTGTGATGATGATGCTGATGGGGATGGGATCAAGAATGTT GAGGACAACTGCCGGCTGTTCCCCAACAAAGACCAGCAGAACTCAGATACAGATTCATTTGGTGATGCC TGTGACAATTGCCCCAACGTTCCCAACAATGACCAGAAGGACACAGATGGCAATGGGGAAGGAGATGCC TGTGACAACGACGTGGATGGGGATGGCATCCCCAATGGATTGGACAATTGCCCTAAAGTCCCCAACCCA CTACAGACAGACAGGGATGAGGACGGGGTGGGAGATGCTTGCGACAGCTGCCCTGAAATGAGCAATCCT ACCCAGACAGATGCAGACAGCGACCTGGTGGGGGATGTCTGTGATACTAATGAAGACAGCGATGGGGAT GGGCATCAGGACACCAAGGACAACTGCCCACAGCTGCCAAATAGCTCCCAGCTGGACTCTGATAACGAT GGACTTGGAGATGAGTGTGATGGGGATGATGACAATGATGGCATCCCAGATTATGTGCCTCCTGGTCCC GATAACTGCCGCCTGGTACCCAATCCCAATCAGAAGGACTCAGATGGCAATGGCGTTGGTGATGTGTGT GAGGATGACTTTGACAATGATGCTGTGGTCGACCCCCTGGATGTGTGTCCTGAAAGTGCAGAGGTAACG CTTACGGATTTTCGGGCCTATCAGACCGTCGTCCTGGATCCTGAGGGTGATGCTCAGATTGACCCAAAC TGGGTTGTGCTCAACCAGGGCATGGAAATCGTTCAGACCATGAACAGTGACCCTGGCTTGGCAGTTGGA TACACGGCCTTCAATGGTGTGGACTTTGAAGGCACCTTCCATGTGAACACAGTGACTGATGATGACTAC GCAGGCTTTCTCTTCAGTTATCAAGACAGTGGCCGCTTCTACGTAGTCATGTGGAAGCAGACCGAGCAG ACCTACTGGCAGGCTACACCCTTCCGGGCGGTTGCCCAGCCCGGGCTGCAGCTCAAGGCAGTGACATCA GTGTCTGGCCCAGGTGAGCACCTCCGAAATGCCCTGTGGCATACTGGCCACACCCCTGATCAGGTACGA CTGCTGTGGACAGACCCACGAAATGTGGGCTGGCGGGACAAGACCTCCTATCGCTGGCAGCTTCTGCAC CGGCCTCAAGTTGGCTACATTCGGGTGAAGCTCTATGAGGGACCCCAGCTTGTGGCGGATTCTGGGGTG ATCATTGACACATCCATGCGAGGGGGGCGTCTTGGTGTATTCTGCTTCTCCCAAGAAAACATAATTTGG TCCAATCTCCAGTATCGATGCAATGACACAGTGCCTGAGGACTTTGAGCCATTCCGGAGGCAGCTGCTC CAGGGAAGGGTGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001252607
Insert Size	2844 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252607.1
RefSeq Size	3224 bp
RefSeq ORF	2844 bp
Locus ID	7059
UniProt ID	P49746
Protein Families	Druggable Genome
Protein Pathways	ECM-receptor interaction, Focal adhesion, TGF-beta signaling pathway
MW	103.3 kDa
Gene Summary	The protein encoded by this gene belongs to the thrombospondin family. Thrombospondin family members are adhesive glycoproteins that mediate cell-to-cell and cell-to-matrix interactions. This protein forms a pentameric molecule linked by a single disulfide bond. This gene shares a common promoter with metaxin 1. Alternate splicing results in coding and non-coding transcript variants. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.

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