SOAT 1 (SOAT1) (NM_001252512) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ACACT; ACAT; ACAT-1; ACAT1; SOAT; STAT
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332198 representing NM_001252512. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAAGGAAGTTGGCAGTCACTTTGATGATTTTGTGACCAATCTCATTGAAAAGTCAGCATCATTAGAT AATGGTGGGTGCGCTCTCACAACCTTTTCTGTTCTTGAAGGAGAGAAAAACAACCATAGAGCGAAGGAT TTGAGAGCACCTCCAGAACAAGGAAAGATTTTTATTGCAAGGCGCTCTCTCTTAGATGAACTGCTTGAA GTGGACCACATCAGAACAATATATCACATGTTTATTGCCCTCCTCATTCTCTTTATCCTCAGCACACTT GTAGTAGATTACATTGATGAAGGAAGGCTGGTGCTTGAGTTCAGCCTCCTGTCTTATGCTTTTGGCAAA TTTCCTACCGTTGTTTGGACCTGGTGGATCATGTTCCTGTCTACATTTTCAGTTCCCTATTTTCTGTTT CAACATTGGGCCACTGGCTATAGCAAGAGTTCTCATCCGCTGATCCGTTCTCTCTTCCATGGCTTTCTT TTCATGATCTTCCAGATTGGAGTTCTAGGTTTTGGACCAACATATGTTGTGTTAGCATATACACTGCCA CCAGCTTCCCGGTTCATCATTATATTCGAGCAGATTCGTTTTGTAATGAAGGCCCACTCATTTGTCAGA GAGAACGTGCCTCGGGTACTAAATTCAGCTAAGGAGAAATCAAGCACTGTTCCAATACCTACAGTCAAC CAGTATTTGTACTTCTTATTTGCTCCTACCCTTATCTACCGTGACAGCTATCCCAGGAATCCCACTGTA AGATGGGGTTATGTCGCTATGAAGTTTGCACAGGTCTTTGGTTGCTTTTTCTATGTGTACTACATCTTT GAAAGGCTTTGTGCCCCCTTGTTTCGGAATATCAAACAGGAGCCCTTCAGCGCTCGTGTTCTGGTCCTA TGTGTATTTAACTCCATCTTGCCAGGTGTGCTGATTCTCTTCCTTACTTTTTTTGCCTTTTTGCACTGC TGGCTCAATGCCTTTGCTGAGATGTTACGCTTTGGTGACAGGATGTTCTATAAGGATTGGTGGAACTCC ACGTCATACTCCAACTATTATAGAACCTGGAATGTGGTGGTCCATGACTGGCTATATTACTATGCTTAC AAGGACTTTCTCTGGTTTTTCTCCAAGAGATTCAAATCTGCTGCCATGTTAGCTGTCTTTGCTGTATCT GCTGTAGTACACGAATATGCCTTGGCTGTTTGCTTGAGCTTTTTCTATCCCGTGCTCTTCGTGCTCTTC ATGTTCTTTGGAATGGCTTTCAACTTCATTGTCAATGATAGTCGGAAAAAGCCGATTTGGAATGTTCTG ATGTGGACTTCTCTTTTCTTGGGCAATGGAGTCTTACTCTGCTTTTATTCTCAAGAATGGTATGCACGT CAGCACTGTCCTCTGAAAAATCCCACATTTTTGGATTATGTCCGGCCACGTTCCTGGACTTGTCGTTAC GTGTTTTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001252512
Insert Size	1458 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252512.1
RefSeq Size	6812 bp
RefSeq ORF	1458 bp
Locus ID	6646
UniProt ID	P35610
Protein Families	Transmembrane
Protein Pathways	Steroid biosynthesis
MW	57.2 kDa
Gene Summary	The protein encoded by this gene belongs to the acyltransferase family. It is located in the endoplasmic reticulum, and catalyzes the formation of fatty acid-cholesterol esters. This gene has been implicated in the formation of beta-amyloid and atherosclerotic plaques by controlling the equilibrium between free cholesterol and cytoplasmic cholesteryl esters. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (3) lacks an internal exon in the 5' region compared to variant 1. This results in translation initiation from an in-frame, downstream start codon, and an isoform (3) with a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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