ThPok (ZBTB7B) (NM_001252406) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	c-KROX; CKROX; hcKROX; THPOK; ZBTB15; ZFP-67; ZFP67; ZNF857B
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332196 representing NM_001252406. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTTACAGCCTGGTCCTCATCCTCCCTCACCCCAAGCTGCTGCTCCTGGAGAAGCCTGGCCAGGCCCC TCTCAGGCTCCCTGGCAGAGCCTAGAGGAGAAGATGGGGAGCCCCGAGGATGACCTGATTGGGATTCCA TTCCCGGACCACAGCAGTGAGCTCCTGAGCTGCCTCAATGAGCAGCGCCAGCTGGGCCACCTATGTGAC CTCACCATCCGGACGCAGGGCCTTGAATACCGCACCCACAGGGCTGTGCTAGCTGCCTGTAGCCACTAC TTCAAGAAGCTTTTCACTGAGGGCGGTGGCGGAGCTGTCATGGGGGCCGGGGGTAGCGGGACGGCCACT GGGGGAGCAGGGGCCGGTGTGTGTGAGCTGGACTTTGTAGGGCCAGAGGCACTAGGCGCCCTCCTTGAA TTTGCCTATACAGCCACACTGACCACCAGCAGCGCCAACATGCCAGCTGTGCTCCAGGCTGCCCGCCTG CTGGAGATCCCGTGTGTCATCGCTGCTTGCATGGAGATTCTGCAGGGCAGTGGGCTAGAAGCTCCCAGC CCGGACGAGGATGACTGTGAGCGAGCCCGCCAGTATCTGGAGGCCTTTGCCACAGCCACGGCCTCTGGA GTTCCCAATGGTGAAGACAGTCCTCCACAGGTGCCCCTCCCACCACCTCCGCCACCGCCACCTCGGCCT GTTGCCCGCCGCAGCCGCAAGCCCCGGAAAGCTTTCCTGCAAACCAAGGGGGCCAGAGCAAACCACCTA GTCCCTGAGGTGCCCACAGTGCCCGCCCATCCCTTGACCTATGAGGAGGAGGAGGTGGCGGGCAGAGTG GGCAGCAGTGGGGGCAGTGGGCCGGGGGACAGCTACAGCCCTCCCACAGGAACTGCCTCCCCTCCTGAG GGTCCCCAGAGCTACGAACCCTATGAGGGTGAGGAAGAAGAAGAGGAGCTGGTATATCCCCCAGCCTAT GGGCTGGCGCAGGGTGGCGGGCCCCCGCTGTCCCCAGAGGAGCTGGGCTCAGATGAGGATGCCATCGAT CCTGACCTGATGGCCTACCTAAGCTCCCTGCACCAGGACAACCTGGCACCAGGCCTGGACAGCCAAGAC AAGCTGGTGCGCAAACGCCGCTCCCAGATGCCTCAGGAGTGCCCTGTCTGCCACAAGATCATCCATGGG GCAGGCAAACTGCCTCGCCACATGAGGACCCACACAGGCGAGAAGCCCTTTGCCTGCGAGGTCTGCGGT GTTCGATTCACCAGGAACGACAAGCTGAAGATCCACATGCGGAAGCACACGGGAGAGCGCCCCTACTCA TGCCCGCACTGCCCAGCCCGCTTCCTGCACAGCTACGACCTCAAGAACCACATGCACCTGCACACAGGG GACCGGCCCTATGAGTGCCACCTGTGCCACAAGGCTTTCGCCAAGGAGGACCACCTGCAGCGCCACCTC AAAGGCCAGAACTGCCTGGAGGTGCGCACCCGACGGCGCCGCAAGGACGATGCACCACCCCACTACCCA CCACCCTCTACCGCTGCTGCATCCCCCGCTGGCCTCGACCTCTCCAATGGCCACCTGGACACCTTCCGC CTCTCTCTAGCTCGATTCTGGGAGCAGTCAGCCCCCACTGGGCCCCCGGTCTCTACCCCAGGGCCCCCT GATGACGATGAGGAGGAAGGGGCACCCACCACACCCCAGGCTGAAGGTGCCATGGAGTCCTCTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252406
Insert Size	1722 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252406.2
RefSeq Size	4030 bp
RefSeq ORF	1722 bp
Locus ID	51043
UniProt ID	O15156
Protein Families	Transcription Factors
MW	61.6 kDa
Gene Summary	This gene encodes a zinc finger-containing transcription factor that acts as a key regulator of lineage commitment of immature T-cell precursors. It is necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. It also functions as a transcriptional repressor of type I collagen genes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (3) represents the longest transcript and encodes the longer isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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