PASK (NM_001252122) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PASKIN; STK37
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332178 representing NM_001252122. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGGACGGGGGCTTAACAGCCTTTGAAGAGGACCAGAGATGCCTTTCCCAGAGCCTCCCCTTGCCA GTGTCAGCAGAGGGCCCAGCTGCACAGACCACTGCTGAGCCCAGCAGGTCGTTTTCCTCAGCCCACAGA CACCTGAGCAGAAGGAATGGGCTTTCCAGACTCTGCCAGAGCAGGACAGCGCTCTCTGAAGACAGATGG AGCTCCTATTGTCTATCATCACTGGCTGCCCAGAATATTTGTACAAGTAAACTGCACTGCCCTGCTGCC CCTGAGCACACGGACCCGTCCGAACCGCGGGGCAGTGTGTCCTGCTGCTCCCTGCTGCGGGGACTGTCC TCAGGGTGGTCCTCACCTCTGCTTCCGGCCCCTGTGTGCAACCCTAACAAGGCCATCTTCACGGTGGAT GCCAAGACCACAGAGATCCTGGTTGCTAACGACAAAGCTTGCGGGCTCCTGGGGTACAGCAGCCAGGAC CTGATTGGCCAGAAGCTCACGCAGTTCTTTCTGAGGTCAGATTCTGATGTGGTGGAGGCCCTCAGCGAG GAGCACATGGAGGCCGACGGCCACGCTGCGGTGGTGTTTGGCACGGTGGTGGACATCATCAGCCGTAGT GGGGAGAAGATTCCAGTGTCTGTGTGGATGAAGAGGATGCGGCAGGAGCGCCGCCTATGCTGCGTGGTG GTCCTGGAGCCCGTGGAGAGGGTCTCGACCTGGGTCGCTTTCCAGAGCGATCTCCCTCCTTCTGGCCAG CACATCCCAAAGAATCTCAAGATTCAGAGGTCTGTTGGAAGAGCCAGGGACGGTACCACCTTCCCTCTG AGCTTAAAGCTGAAATCCCAACCCAGCAGCGAGGAGGCGACCACCGGTGAGGCGGCCCCTGTGAGCGGC TACCGGGCATCTGTCTGGGTGTTCTGCACCATCAGTGGCCTCATCACCCTCCTGCCGGATGGGACCATC CACGGCATCAACCACAGCTTCGCGCTGACACTGTTTGGTTACGGAAAGACGGAGCTCCTGGGCAAGAAT ATCACTTTCCTGATTCCTGGTTTCTACAGCTACATGGACCTTGCGTACAACAGCTCATTACAGCTCCCA GACCTGGCCAGCTGCCTGGACGTCGGCAATGAGAGTGGGTGTGGGGAGAGAACCTTGGACCCGTGGCAG GGCCAGGACCCAGCTGAGGGGGGCCAGGATCCAAGGATTAATGTCGTGCTTGCTGGTGGCCACGTTGTG CCCCGAGATGAGATCCGGAAGCTGATGGAAAGCCAAGACATCTTCACCGGGACTCAGACTGAGCTGATT GCTGGAGGCCAGCTCCTTTCCTGCCTCTCACCTCAGCCTGCTCCAGGGGTGGACAATGTCCCAGAAGGA AGCCTGCCAGTGCACGGTGAACAGGCGCTGCCCAAGGACCAGCAAATCACTGCCTTGGGGAGAGAGGAA CCTGTGGCAATAGAGAGCCCCGGACAGGATCTTCTGGGAGAAAGCAGGTCTGAACCAGTGGATGTGAAG CCATTTGCTTCCTGCGAAGATTCTGAAGCTCCAGTCCCAGCTGAGGATGGGGGCAGTGATGCTGGCATG TGTGGCCTGTGTCAGAAGGCCCAGCTAGAGCGGATGGGAGTCAGTGGTCCCAGCGGTTCAGACCTTTGG GCTGGGGCTGCCGTGGCCAAGCCCCAGGCCAAGGGTCAGCTGGCGGGGGGCAGCCTCCTGATGCACTGC CCTTGCTATGGGAGTGAATGGGGCTTGTGGTGGCGAAGCCAGGACTTGGCCCCCAGCCCCTCTGGGATG GCAGGCCTCTCGTTTGGGACACCTACTCTAGATGAGCCGTGGCTGGGAGTGGAAAACGACCGAGAAGAG CTGCAGACCTGCTTGATTAAGGAGCAGCTGTCCCAGTTGAGCCTTGCAGGAGCCCTGGATGTCCCCCAC GCCGAACTCGTTCCGACAGAGTGCCAGGCTGTCACCGCTCCTGTGTCGTCCTGCGATCTGGGAGGCAGA GACCTGTGCGGTGGCTGCACGGGCAGCTCCTCAGCCTGCTATGCCTTGGCCACGGACCTCCCTGGGGGC CTGGAAGCAGTGGAGGCCCAGGAGGTTGATGTGAATTCGTTTTCCTGGAACCTCAAGGAACTCTTTTTC AGTGACCAGACAGACCAAACGTCATCAAATTGTTCCTGTGCTACGTCTGAACTCAGAGAGACACCCTCT TCCTTGGCAGTGGGCTCCGATCCAGATGTAGGCAGTCTCCAGGAACAGGGGTCGTGTGTCCTGGATGAC AGGGAGCTGTTACTACTGACCGGCACCTGTGTTGACCTTGGCCAAGGCCGACGGTTCCGGGAGAGCTGT GTGGGACATGATCCAACAGAACCGCTTGAGGTTTGTTTGGTGTCCTCTGAGCATTATGCAGCAAGCGAC AGAGAAAGCCCAGGACACGTTCCTTCCACGTTGGATGCTGGCCCTGAGGACACGTGCCCATCAGCAGAG GAGCCAAGGCTGAACGTCCAGGTCACCTCCACGCCCGTGATCGTGATGCGCGGGGCTGCTGGCCTGCAG CGGGAGATCCAGGAGGGTGCCTACTCCGGGAGCTGCTACCATCGAGATGGCTTACGGCTGAGTATACAG TTTGAGGTGAGGCGGGTGGAGCTCCAGGGCCCCACACCTCTGTTCTGCTGCTGGCTGGTGAAAGACCTC CTCCACAGCCAACGCGACTCAGCCGCCAGGACCCGCCTGTTCCTTGCCAGCCTGCCCGGCTCCACCCAC TCTACCGCTGCTGAGCTCACCGGACCCAGCCTGGTGGAAGTGCTCAGAGCCAGACCCTGGTTTGAGGAG CCCCCCAAGGCTGTGGAACTGGAGGGGTTGGCGGCCTGTGAGGGCGAGTACTCCCAAAAGTACAGTACC ATGAGCCCGCTGGGCAGTGGGGCCTTCGGCTTCGTGTGGACTGCTGTGGACAAGGAAAAAAACAAGGAG GTGGTGGTGAAGTTTATTAAGAAGGAGAAGGTCTTGGAGGATTGTTGGATTGAGGATCCCAAACTTGGG AAAGTTACTTTAGAGATCGCAATTCTATCCAGGGTGGAGCACGCCAATATCATCAAGGTATTGGATATA TTTGAAAACCAAGGGTTCTTCCAGCTTGTGATGGAGAAGCACGGCTCCGGCCTAGACCTCTTCGCTTTC ATCGACCGCCACCCCAGGCTGGATGAGCCCCTGGCGAGCTACATCTTCCGACAACTAGTGTCAGCAGTG GGATACCTGCGCTTGAAGGACATCATCCACCGTGACATCAAGGATGAGAACATCGTGATCGCCGAGGAC TTCACAATCAAGCTGATAGACTTTGGCTCGGCCGCCTACTTGGAAAGGGGAAAATTATTTTATACTTTT TGTGGGACCATCGAGTACTGTGCACCGGAAGTTCTCATGGGGAATCCCTACAGAGGGCCGGAGCTGGAG ATGTGGTCTCTGGGAGTCACTCTGTACACGCTGGTCTTTGAGGAGAACCCCTTCTGTGAGCTGGAGGAG ACCGTGGAGGCTGCCATACACCCGCCATACCTGGTGTCCAAAGAACTCATGAGCCTTGTGTCTGGGCTG CTGCAGCCAGTCCCTGAGAGACGCACCACCTTGGAGAAGCTGGTGACAGACCCGTGGGTAACACAGCCT GTGAATCTTGCTGACTATACATGGGAAGAGGTGTTTCGAGTAAACAAGCCAGAAAGTGGAGTTCTGTCC GCTGCGAGCCTGGAGATGGGGAACAGGAGCCTGAGTGATGTGGCCCAGGCTCAGGAGCTTTGTGGGGGC CCCGTTCCAGGCGAGGCTCCTAATGGCCAAGGCTGTTTGCATCCCGGGGATCCCCGTCTGCTGACCAGC TAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252122
Insert Size	3867 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252122.1
RefSeq Size	4483 bp
RefSeq ORF	3867 bp
Locus ID	23178
UniProt ID	Q96RG2
Protein Families	Druggable Genome, Protein Kinase, Stem cell - Pluripotency
MW	139.3 kDa
Gene Summary	This gene encodes a member of the serine/threonine kinase family that contains two PAS domains. Expression of this gene is regulated by glucose, and the encoded protein plays a role in the regulation of insulin gene expression. Downregulation of this gene may play a role in type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (4) uses two alternate splice sites in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (3) is shorter than isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.