ASAP1 (NM_001247996) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332121 representing NM_001247996. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGTTTCAAATTTAAAGAGGATTATGGCCCAGAAATGGATGCCGGACCAGATCTCTGTCTCGGAGTTC ATCGCCGAGACCACCGAGGACTACAACTCGCCCACCACGTCCAGCTTCACCACGCGGCTGCACAACTGC AGGAACACCGTCACGCTGCTGGAGGAGGCTCTAGACCAAGATAGAACAGCCCTTCAGAAAGTGAAGAAG TCTGTAAAAGCAATATATAATTCTGGTCAAGATCATGTACAAAATGAAGAAAACTATGCACAAGTTCTT GATAAGTTTGGGAGTAATTTTTTAAGTCGAGACAACCCCGACCTTGGCACCGCGTTTGTCAAGTTTTCT ACTCTTACAAAGGAACTGTCCACACTGCTGAAAAATCTGCTCCAGGGTTTGAGCCACAATGTGATCTTC ACCTTGGATTCTTTGTTAAAAGGAGACCTAAAGGGAGTCAAAGGAGATCTCAAGAAGCCATTTGACAAA GCCTGGAAAGATTATGAGACAAAGTTTACAAAAATTGAGAAAGAGAAAAGAGAGCACGCAAAACAACAT GGGATGATCCGCACAGAGATAACAGGAGCTGAGATTGCGGAAGAAATGGAGAAGGAAAGGCGCCTCTTT CAGCTCCAAATGTGTGAATATCTCATTAAAGTTAATGAAATCAAGACCAAAAAGGGTGTGGATCTGCTG CAGAATCTTATAAAGTATTACCATGCACAGTGCAATTTCTTTCAAGATGGCTTGAAAACAGCTGATAAG TTGAAACAGTACATTGAAAAACTGGCTGCTGATTTATATAATATAAAACAGACCCAGGATGAAGAAAAG AAACAGCTAACTGCACTCCGAGACTTAATAAAATCCTCTCTTCAACTGGATCAGAAAGAAGATTCTCAG AGCCGGCAAGGAGGATACAGCATGCATCAGCTCCAGGGCAATAAGGAATATGGCAGTGAAAAGAAGGGG TACCTGCTAAAGAAAAGTGACGGGATCCGGAAAGTATGGCAGAGGAGGAAGTGTTCAGTCAAGAATGGG ATTCTGACCATCTCACATGCCACATCTAACAGGCAACCAGCCAAGTTGAACCTTCTCACCTGCCAAGTA AAACCTAATGCCGAAGACAAAAAATCTTTTGACCTGATATCACATAATAGAACATATCACTTTCAGGCA GAAGATGAGCAGGATTATGTAGCATGGATATCAGTATTGACAAATAGCAAAGAAGAGGCCCTAACCATG GCCTTCCGTGGAGAGCAGAGTGCGGGAGAGAACAGCCTGGAAGACCTGACAAAAGCCATTATTGAGGAT GTCCAGCGGCTCCCAGGGAATGACATTTGCTGCGATTGTGGCTCATCAGAACCCACCTGGCTTTCAACC AACTTGGGTATTTTGACCTGTATAGAATGTTCTGGCATCCATAGGGAAATGGGGGTTCATATTTCTCGC ATTCAGTCTTTGGAACTAGACAAATTAGGAACTTCTGAACTCTTGCTGGCCAAGAATGTAGGAAACAAT AGTTTTAATGATATTATGGAAGCAAATTTACCCAGCCCCTCACCAAAACCCACCCCTTCAAGTGATATG ACTGTACGAAAAGAATATATCACTGCAAAGTATGTAGATCATAGGTTTTCAAGGAAGACCTGTTCAACT TCATCAGCTAAACTAAATGAATTGCTTGAGGCCATCAAATCCAGGGATTTACTTGCACTAATTCAAGTC TATGCAGAAGGGGTAGAGCTAATGGAACCACTGCTGGAACCTGGGCAGGAGCTTGGGGAGACAGCCCTT CACCTTGCCGTCCGAACTGCAGATCAGACATCTCTCCATTTGGTTGACTTCCTTGTACAAAACTGTGGG AACCTGGATAAGCAGACGGCCCTGGGAAACACAGTTCTACACTACTGTAGTATGTACAGTAAACCTGAG TGTTTGAAGCTTTTGCTCAGGAGCAAGCCCACTGTGGATATAGTTAACCAGGCTGGAGAAACTGCCCTA GACATAGCAAAGAGACTAAAAGCTACCCAGTGTGAAGATCTGCTTTCCCAGGCTAAATCTGGAAAGTTC AATCCACACGTCCACGTAGAATATGAGTGGAATCTTCGACAGGAGGAGATAGATGAGAGCGATGATGAT CTGGATGACAAACCAAGCCCTATCAAGAAAGAGCGCTCACCCAGACCTCAGAGCTTCTGCCACTCCTCC AGCATCTCCCCCCAGGACAAGCTGGCACTGCCAGGATTCAGCACTCCAAGGGACAAACAGCGGCTCTCC TATGGAGCCTTCACCAACCAGATCTTCGTTTCCACAAGCACAGACTCGCCCACATCACCAACCACGGAG GCTCCCCCTCTGCCTCCTAGGAACGCCGGGAAAGGTCCAACTGGCCCACCTTCAACACTCCCTCTAAGC ACCCAGACCTCTAGTGGCAGCTCCACCCTATCCAAGAAGAGGCCTCCTCCCCCACCACCCGGACACAAG AGAACCCTATCCGACCCTCCCAGCCCACTACCTCATGGGCCCCCAAACAAAGGCGCAGTTCCTTGGGGT AACGATGGGGGTCCATCCTCTTCAAGTAAGACTACAAACAAGTTTGAGGGACTATCCCAGCAGTCGAGC ACCAGTTCTGCAAAGACTGCCCTTGGCCCAAGAGTTCTTCCTAAACTACCTCAGAAAGTGGCACTAAGG AAAACAGATCATCTCTCCCTAGACAAAGCCACCATCCCGCCCGAAATCTTTCAGAAATCATCACAGTTG GCAGAGTTGCCACAAAAGCCACCACCTGGAGACCTGCCCCCAAAGCCCACAGAACTGGCCCCCAAGCCC CAAATTGGAGATTTGCCGCCTAAGCCAGGAGAACTGCCCCCCAAACCACAGCTGGGGGACCTGCCACCC AAACCCCAACTCTCAGACTTACCTCCCAAACCACAGATGAAGGACCTGCCCCCCAAACCACAGCTGGGA GACCTGCTAGCAAAATCCCAGACTGGAGATGTCTCACCCAAGGCTCAGCAACCCTCTGAGGTCACACTG AAGTCACACCCATTGGATCTATCCCCAAATGTGCAGTCCAGAGACGCCATCCAAAAGCAAGCATCTGAA GACTCCAACGACCTCACGCCTACTCTGCCAGAGACGCCCGTACCACTGCCCAGAAAAATCAATACGGGG AAAAATAAAGTGAGGCGAGTGAAGACCATTTATGACTGCCAGGCAGACAACGATGACGAGCTCACATTC ATCGAGGGAGAAGTGATTATCGTCACAGGGGAAGAGGACCAGGAGTGGTGGATTGGCCACATCGAAGGA CAGCCTGAAAGGAAGGGGGTCTTTCCAGTGTCCTTTGTTCATATCCTGTCTGACTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001247996
Insert Size	3369 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001247996.1
RefSeq Size	6361 bp
RefSeq ORF	3369 bp
Locus ID	50807
UniProt ID	Q9ULH1
Protein Pathways	Endocytosis, Fc gamma R-mediated phagocytosis
MW	124.8 kDa
Gene Summary	This gene encodes an ADP-ribosylation factor (ARF) GTPase-activating protein. The GTPase-activating activity is stimulated by phosphatidylinositol 4,5-biphosphate (PIP2), and is greater towards ARF1 and ARF5, and lesser for ARF6. This gene maybe involved in regulation of membrane trafficking and cytoskeleton remodeling. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] Transcript Variant: This variant (2) contains an additional exon (with an in-frame AUG) in the 5' region compared to variant 1. This results in translation initiation from an alternate start site, and a shorter isoform (2) with a distinct N-terminus compared to isoform 1.

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