Myosin Phosphatase (PPP1R12A) (NM_001244992) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GUBS; M130; MBS; MYPT1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332112 representing NM_001244992. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAAGATGGCGGACGCGAAGCAGAAGCGGAACGAGCAGCTGAAACGCTGGATCGGCTCCGAGACGGAC CTCGAGCCTCCGGTGGTGAAGCGCCAGAAGACCAAGGTGAAGTTCGACGATGGCGCCGTCTTCCTGGCT GCTTGCTCCAGCGGCGACACGGACGAGGTCCTCAAGCTGCTGCACCGCGGCGCCGACATCAATTACGCC AATGTGGACGGACTCACTGCCCTGCACCAGGCTTGCATTGATGACAATGTTGATATGGTGAAGTTTCTG GTAGAAAATGGAGCAAATATTAATCAACCTGATAATGAAGGCTGGATACCACTACATGCAGCAGCTTCC TGTGGATATCTTGATATTGCAGAGTTTTTGATTGGTCAAGGAGCACATGTAGGGGCTGTCAACAGTGAA GGAGATACACCTTTAGATATTGCGGAGGAGGAGGCAATGGAAGAGCTACTTCAAAATGAAGTTAATCGG CAAGGGGTTGATATAGAAGCAGCTCGAAAGGAAGAAGAACGGATCATGCTTAGAGATGCCAGGCAGTGG CTAAATAGTGGTCATATAAATGATGTCCGGCATGCAAAATCTGGAGGTACAGCACTTCACGTTGCAGCT GCTAAAGGCTATACGGAAGTTTTAAAACTTTTAATACAGGCAGGCTATGATGTTAATATTAAAGACTAT GATGGCTGGACACCTCTTCATGCTGCAGCTCATTGGGGTAAAGAAGAAGCATGTCGAATTTTAGTGGAC AATCTGTGTGATATGGAGATGGTCAACAAAGTGGGCCAAACAGCCTTTGATGTAGCAGATGAAGACATT TTAGGATATTTAGAAGAGTTGCAAAAGAAACAAAATCTGCTCCATAGTGAAAAACGGGACAAGAAATCT CCACTAATTGAATCAACAGCAAATATGGACAATAATCAGTCACAGAAGACCTTTAAAAACAAAGAGACG TTGATTATTGAACCAGAGAAAAATGCATCCCGTATTGAATCTCTGGAACAAGAAAAGGTTGATGAAGAA GAAGAAGGAAAGAAGGATGAGTCTAGCTGCTCTAGTGAAGAAGATGAGGAAGATGACTCGGAATCAGAA GCTGAAACAGATAAGACAAAACCCCTGGCTTCTGTAACTAATGCCAACACTTCTAGTACACAAGCAGCT CCTGTAGCTGTTACAACACCTACTGTGTCATCAGGTCAAGCAACACCTACATCACCTATTAAAAAGTTT CCAACCACAGCTACAAAAATTTCTCCCAAAGAAGAAGAGAGAAAAGATGAGTCTCCTGCAACTTGGAGG TTAGGACTTAGAAAGACGGGCAGCTATGGTGCACTTGCTGAAATCACAGCATCTAAAGAGGGTCAGAAA GAAAAAGATACTGCAGGTGTTACACGTTCAGCTTCAAGTCCCAGACTTTCCTCCTCTTTGGATAATAAA GAAAAGGAGAAAGATAGTAAAGGAACTAGGCTTGCATATGTTGCACCTACAATACCAAGACGACTAGCC AGTACATCTGACATTGAAGAGAAAGAAAACAGAGATTCTTCAAGTTTGCGAACAAGTAGTTCATATACA AGGAGAAAATGGGAAGATGATCTTAAAAAAAATAGCTCAGTTAATGAAGGATCAACGTATCATAAAAGT ACCTCAAATCGTTTGTGGGCTGAGGATAGTACTGAGAAAGAAAAGGACAGTGTTCCTACGGCAGTGACC ATTCCTGTTGCTCCAACTGTTGTAAATGCTGCAGCTTCTACCACAACCCTGACTACAACTACTGCTGGC ACTGTCTCCTCCACAACAGAGGTCAGGGAGAGACGCAGATCATACCTCACTCCTGTTAGGGATGAAGAG TCTGAATCCCAAAGAAAAGCAAGATCTAGACAAGCAAGACAATCTAGAAGATCAACACAGGGAGTGACA TTAACTGATCTTCAAGAAGCTGAGAAAACAATAGGAAGAAGTCGTTCTACCCGAACCAGAGAACAAGAA AATGAAGAAAAAGAAAAAGAGGAAAAAGAGAAACAAGATAAAGAGAAACAAGAAGAAAAGAAGGAGTCA GAAACATCTAGAGAAGATGAATATAAACAAAAGTACTCCAGAACGTATGATGAGACTTACCAGCGTTAT AGGCCAGTATCAACTTCAAGTTCAACCACTCCATCCTCTTCACTTTCTACTATGAGCAGTTCACTGTAT GCTTCAAGTCAACTAAACAGGCCAAATAGTCTTGTAGGCATAACTTCTGCTTACTCCAGAGGAATAACA AAAGAAAATGAAAGAGAGGGAGAAAAAAGAGAAGAGGAGAAAGAAGGAGAAGATAAATCACAACCTAAA TCAATCAGAGAACGACGACGACCAAGAGAGAAAAGAAGATCTACAGGAGTTTCATTTTGGACACAAGAT AGTGATGAAAATGAACAAGAACAACAATCAGACACAGAAGAGGGATCCAATAAGAAAGAAACTCAGACG GATTCCATTTCTAGATATGAAACCAGTTCTACATCAGCTGGTGATCGATATGATTCCTTGCTGGGTCGC TCTGGATCATACAGTTACTTAGAAGAAAGAAAACCTTACAGCAGCAGGCTAGAAAAGGATGACTCAACT GACTTTAAAAAGCTTTATGAACAAATTCTAGCTGAAAATGAAAAGCTGAAGGCACAGCTACATGATACA AATATGGAACTAACAGATCTTAAATTACAGTTGGAAAAGGCCACCCAGAGACAAGAAAGATTTGCTGAT AGATCACTGTTGGAAATGGAAAAAAGGGAACGAAGAGCTCTAGAAAGAAGAATATCTGAAATGGAAGAA GAGCTCAAAATGTTACCAGACCTAAAAGCAGACAACCAGAGGCTAAAGGATGAAAATGGGGCCTTGATC AGAGTTATAAGCAAACTTTCCAAATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001244992
Insert Size	2925 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001244992.1
RefSeq Size	5311 bp
RefSeq ORF	2925 bp
Locus ID	4659
UniProt ID	O14974
Protein Families	Druggable Genome
Protein Pathways	Focal adhesion, Long-term potentiation, Regulation of actin cytoskeleton, Vascular smooth muscle contraction
MW	109.7 kDa
Gene Summary	Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009] Transcript Variant: This variant (5) lacks an exon in the coding region, compared to variant 1. The encoded isoform (d) is shorter than isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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