KIAA0586 (NM_001244191) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	JBTS23; SRTD14; Talpid3
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332068 representing NM_001244191. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGTGTCAGAAAGTGATTTTTCTAAAGACGTTGCAGTGCAAGTGTTGCCTTTGGATAAAATAGAAGAG AACAACAAGCAAAAAGCAAATGACATCTTCATTTCTCAGTATACAATGGGACAGAAAGATGCTCTAAGA ACAGTTTTAAAGCAAAAAGCTCAAAGCATGCCTGTTTTTAAGGAAGTAAAGGTACATCTGTTAGAAGAT GCAGGCATAGAGAAGGATGCTGTTACTCAGGAGACTAGAATTTCACCCAGTGGAATTGATTCAGCTACA ACCGTGGCTGCAGCAACTGCTGCTGCCATTGCAACCGCAGCTCCGTTGATAAAGGTGCAGAGTGATTTG GAAGCAAAAGTCAATTCTGTTACAGAATTACTTAGTAAATTACAGGAGACTGATAAACACCTGCAACGT GTTACAGAGCAGCAAACAAGCATTCAGAGGAAACAAGAGAAATTACATTGTCATGATCACGAAAAGCAA ATGAATGTGTTTATGGAGCAGCACATAAGGCATCTTGAAAAGTTACAACAACAACAAATAGATATTCAG ACTCATTTTATTAGTGCTGCACTCAAGACTAGTAGTTTTCAGCCTGTTAGTATGCCCTCCTCCAGAGCA GTGGAAAAGTATTCCGTAAAACCAGAACACCCTAATCTTGGTAGCTGTAATCCATCTTTATATAACACA TTTGCTTCCAAACAAGCACCTTTAAAAGAAGTTGAAGATACGAGTTTTGATAAACAGAAATCTCCTTTG GAGACACCAGCACCTCGCAGATTTGCTCCTGTACCTGTTTCAAGGGATGATGAACTATCAAAGAGGGAA AATCTTTTGGAAGAAAAAGAAAATATGGAAGTGTCGTGTCACAGAGGAAATGTAAGACTATTGGAACAA ATTTTGAATAATAATGATTCTTTGACAAGAAAAAGTGAATCATCAAACACCACCTCACTAACTAGGTCA AAAATAGGATGGACTCCTGAGAAAACAAACAGATTTCCTTCCTGTGAAGAGCTAGAAACAACTAAAGTG ACTATGCAGAAGTCTGATGATGTTCTTCATGACCTTGGCCAAAAAGAGAAAGAAACAAATAGCATGGTC CAGCCAAAAGAATCTCTGAGTATGTTGAAGCTTCCAGATCTTCCACAGAATTCTGTTAAGCTTCAAACA ACCAATACAACAAGATCTGTATTGAAAGATGCTGAGAAGATTTTGAGAGGAGTACAAAACAATAAAAAA GTACTTGAAGAAAACCTGGAAGCTATTATTCGTGCAAAAGATGGAGCTGCCATGTATTCGCTTATCAAT GCTTTATCTACCAACAGAGAGATGTCAGAGAAAATTAGGATCAGAAAGACAGTGGATGAATGGATTAAA ACTATTTCTGCAGAAATTCAGGATGAACTGTCAAGAACAGATTATGAACAAAAAAGATTTGATCAGAAG AATCAGAGAACCAAGAAAGGTCAGAATATGACTAAAGATATTAGAACCAACACACAAGATAAAACTGTC AACAAATCTGTAATTCCAAGAAAACATTCTCAAAAGCAAATAGAAGAGCATTTTAGAAATCTACCTATG AGGGGCATGCCTGCTTCAAGTTTACAGAAAGAGAGAAAGGAAGGGCTTTTGAAAGCAACCACAGTAATA CAAGATGAAGATTATATGTTACAAGTCTATGGAAAGCCAGTTTATCAGGGCCATCGAAGCACTCTTAAA AAAGGACCATATCTCAGATTTAATTCTCCATCTCCTAAGTCCAGACCACAGAGACCAAAAGTAATAGAA CGAGTTAAAGGCACTAAGGTAAAGTCAATAAGAACACAGACTGACTTCTATGCAACAAAACCTAAGAAG ATGGATTCTAAAATGAAACATTCTGTTCCTGTGTTACCTCATGGCGATCAGCAATATTTGTTCAGCCCA AGTAGAGAAATGCCTACTTTTTCAGGTACATTGGAAGGTCATCTGATTCCTATGGCAATTCTTTTAGGA CAAACCCAAAGTAATAGTGATACCATGCCACCTGCTGGAGTGATTGTCAGCAAGCCACACCCTGTAACT GTGACTACTTCTATTCCTCCATCATCTCGAAAAGTAGAAACTGGAGTAAAGAAACCTAACATAGCCATT GTAGAAATGAAGTCAGAAAAAAAGGATCCTCCTCAGCTTACTGTGCAGGTATTACCCAGTGTAGATATT GACAGCATTTCAAATAGTAGTGCTGATGTCCTTTCACCTCTGTCTAGCCCCAAAGAAGCATCTCTTCCT CCTGTGCAAACTTGGATAAAGACTCCAGAAATTATGAAGGTAGATGAAGAAGAGGTGAAGTTTCCAGGA ACTAACTTTGATGAAATAATCGATGTCATACAGGAAGAAGAAAAATGTGATGAAATTCCAGACTCTGAA CCAATTCTGGAGTTTAACAGAAGTGTTAAAGCTGATTCTACAAAATATAATGGTCCTCCATTTCCGCCA GTTGCTTCTACTTTTCAGCCCACTGCTGATATTCTGGATAAAGTAATTGAGAGAAAAGAAACACTGGAA AATAGCTTAATTCAATGGGTAGAGCAAGAAATAATGTCAAGAATTATCTCTGGGCTCTTTCCAGTCCAG CAACAGATTGCACCTAGTATCAGTGTTTCAGTCAGTGAGACAAGTGAACCACTGACTTCTGACATTGTG GAAGGAACAAGCAGTGGCGCCCTCCAGCTTTTTGTTGATGCTGGTGTTCCTGTGAACTCAAATGTGATT AAACATTTTGTTAACGAAGCTCTTGCTGAGACCATTGCTGTCATGCTGGGTGACAGAGAAGCAAAGAAG CAAGGTCCTGTTGCTACAGGTGTTTCTGGGGATGCTTCAACAAATGAAACATATTTGCCGGCAAGAGTG TGCACCCCACTGCCTACCCCACAGCCTACGCCTCCTTGCTCACCTTCATCACCTGCTAAGGAGTGTGTT TTGGTAAAGACTCCAGATTCTTCTCCCTGTGATTCGGATCATGATATGGCTTTTCCTGTGAAAGAAATA TGTGCTGAAAAAGGAGATGATATGCCTGCCATCATGCTTGTTAATACTCCAACAGTTACCCCTACTACT ACACCTCCTCCAGCGGCGGCAGTTTTTACCCCAACTTTGTCAGATATTTCCATTGATAAATTGAAGGTA TCAAGCCCAGAGCTTCCCAAGCCATGGGGTGATGGAGACCTGCCACTGGAAGAAGAGAACCCTAACTCA CCTCAAGAAGAACTTCATCCAAGAGCTATTGTAATGTCTGTGGCTAAGGATGAAGAACCAGAGAGTATG GATTTCCCTGCTCAGCCTCCACCTCCAGAGCCAGTTCCCTTTATGCCATTTCCTGCCGGCACCAAGGCC CCTTCCCCCTCACAGATGCCAGGTTCTGATTCATCAACACTGGAGAGCACATTGAGTGTTACTGTCACT GAAACTGAAACTTTAGATAAACCCATCTCTGAAGGAGAGATTTTATTTAGCTGTGGTCAAAAATTGGCC CCCAAGATTTTAGAAGATATAGGACTGTACCTGACAAACCTTAATGATAGCTTATCCAGCACTCTGCAT GATGCCGTTGAAATGGAGGATGATCCTCCTAGTGAAGGGCAAGTGATTAGGATGTCCCATAAAAAATTT CATGCAGATGCAATTCTTTCTTTTGCTAAACAAAACCAGGAGTCAGCAGTTTCCCAGCAAGCAGTCTAT CATTCAGAGGACTTGGAAAACAGTGTGGGTGAACTTAGTGAAGGACAAAGACCCCAGCTAACAGCGGCA GCAGAGAACATCTTAATGGGACATTCTCTCTATATGCAGCCACCTGTCACTAATACACAGTCTTTGGAT CAACAATGTGATCCTAAACCATTATCTCGGCAATTTGACACAGTTTCAGGTAGTATTTATGAAGATTCA TGTGCTAGTCATGGTCCAATGAGTTTGGGAGAATTGGAGTTGGAGCCAAATTCTAAGCTGGTTCTTCCC ACAACACTTCTGACAGCACAAGAAAATGATGTTAATTTACCAGTAGCCGCTGAAGATTTTTCCCAGTAC CAACTAAAGCAAAATCAGGATGTTAAGCAAGTTGAACACAAACCATCACAAAGTTACCTACGTGTTAGA AATAAATCTGATATTGCACCTTCACAGCAACAAGTTTCACCAGGTGATATGGATCGGACACAAATTGAG CTTAATCCGTACCTCACATGTGTATTTTCAGGTGGGAAAGCAGTGCCACTCTCCGCTTCACAGATGCCC CCTGCCAAGATGTCAGTGATGCTGCCGTCAGTGAACCTCGAGGACTGCTCTCAGTCTCTGAGTCTCAGC ACAATGCAGGAGGACATGGAGTCTTCGGGGGCAGATACCTTCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001244191
Insert Size	4392 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001244191.1
RefSeq Size	5609 bp
RefSeq ORF	4392 bp
Locus ID	9786
UniProt ID	Q9BVV6
MW	161.8 kDa
Gene Summary	This gene encodes a conserved centrosomal protein that functions in ciliogenesis and responds to hedgehog signaling. Mutations in this gene causes Joubert syndrome 23. Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Aug 2016] Transcript Variant: This variant (3) lacks an alternate segment in the 5' coding region, uses a downstream start codon, and lacks several alternate in-frame exons, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1.

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