RAG2 (NM_001243785) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RAG-2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332039 representing NM_001243785. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTCTCTGCAGATGGTAACAGTCAGTAATAACATAGCCTTAATTCAGCCAGGCTTCTCACTGATGAAT TTTGATGGACAAGTTTTCTTCTTTGGACAAAAAGGCTGGCCCAAAAGATCCTGCCCCACTGGAGTTTTC CATCTGGATGTAAAGCATAACCATGTCAAACTGAAGCCTACAATTTTCTCTAAGGATTCCTGCTACCTC CCTCCTCTTCGCTACCCAGCCACTTGCACATTCAAAGGCAGCTTGGAGTCTGAAAAGCATCAATACATC ATCCATGGAGGGAAAACACCAAACAATGAGGTTTCAGATAAGATTTATGTCATGTCTATTGTTTGCAAG AACAACAAAAAGGTTACTTTTCGCTGCACAGAGAAAGACTTGGTAGGAGATGTTCCTGAAGCCAGATAT GGTCATTCCATTAATGTGGTGTACAGCCGAGGGAAAAGTATGGGTGTTCTCTTTGGAGGACGCTCATAC ATGCCTTCTACCCACAGAACCACAGAAAAATGGAATAGTGTAGCTGACTGCCTGCCCTGTGTTTTCCTG GTGGATTTTGAATTTGGGTGTGCTACATCATACATTCTTCCAGAACTTCAGGATGGGCTATCTTTTCAT GTCTCTATTGCCAAAAATGACACCATCTATATTTTAGGAGGACATTCACTTGCCAATAATATCCGGCCT GCCAACCTGTACAGAATAAGGGTTGATCTTCCCCTGGGTAGCCCAGCTGTGAATTGCACAGTCTTGCCA GGAGGAATCTCTGTCTCCAGTGCAATCCTGACTCAAACTAACAATGATGAATTTGTTATTGTTGGTGGC TATCAGCTTGAAAATCAAAAAAGAATGATCTGCAACATCATCTCTTTAGAGGACAACAAGATAGAAATT CGTGAGATGGAGACCCCAGATTGGACCCCAGACATTAAGCACAGCAAGATATGGTTTGGAAGCAACATG GGAAATGGAACTGTTTTTCTTGGCATACCAGGAGACAATAAACAAGTTGTTTCAGAAGGATTCTATTTC TATATGTTGAAATGTGCTGAAGATGATACTAATGAAGAGCAGACAACATTCACAAACAGTCAAACATCA ACAGAAGATCCAGGGGATTCCACTCCCTTTGAAGACTCTGAAGAATTTTGTTTCAGTGCAGAAGCAAAT AGTTTTGATGGTGATGATGAATTTGACACCTATAATGAAGATGATGAAGAAGATGAGTCTGAGACAGGC TACTGGATTACATGCTGCCCTACTTGTGATGTGGATATCAACACTTGGGTACCATTCTATTCAACTGAG CTCAACAAACCCGCCATGATCTACTGCTCTCATGGGGATGGGCACTGGGTCCATGCTCAGTGCATGGAT CTGGCAGAACGCACACTCATCCATCTGTCAGCAGGAAGCAACAAGTATTACTGCAATGAGCATGTGGAG ATAGCAAGAGCTCTACACACTCCCCAAAGAGTCCTACCCTTAAAAAAGCCTCCAATGAAATCCCTCCGT AAAAAAGGTTCTGGAAAAATCTTGACTCCTGCCAAGAAATCCTTTCTTAGAAGGTTGTTTGATTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001243785
Insert Size	1584 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001243785.1
RefSeq Size	2652 bp
RefSeq ORF	1584 bp
Locus ID	5897
UniProt ID	P55895
Protein Families	Druggable Genome
Protein Pathways	Primary immunodeficiency
MW	59.2 kDa
Gene Summary	This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (3) includes an alternate exon in the 5' UTR compared to variant 1. Variants 1, 3 and 4 encode the same protein.

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