CEP57 (NM_001243776) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MVA2; PIG8; TSP57
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332036 representing NM_001243776. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTTGACCAGAATTGATAACAGCTTTGCTGAGCCATCAAGGTCTAATGGAAGCATGGTTCGGCATTCT TCATCTCCATATGTAGTATATCCTTCGGATAAGCCTTTCCTTAATAGTGATCTACGACGCTCCCCAAGT AAGCCTACACTTGCCTATCCAGAAAGCAACAGCAGAGCCATATTTTCTGCTCTTAAGAATCTTCAAGAT AAGATTCGACGCTTGGAACTTGAGAGGATTCAGGCAGAAGAAAGTGTGAAAACCTTGTCTAGAGAAACA ATTGAATATAAGAAAGTACTGGATGAACAGATACAAGAAAGGGAGAATTCAAAGAATGAGGAATCAAAG CACAATCAAGAACTGACATCTCAGTTGTTAGCTGCAGAAAATAAATGCAATCTATTAGAAAAACAATTG GAATACATGCGAAATATGATAAAGCATGCCGAAATGGAGAGGACATCTGTCTTAGAGAAACAAGTTTCC CTAGAAAGAGAACGACAACATGATCAAACACATGTTCAGAGCCAACTTGAAAAATTGGATCTTCTTGAA CAGGAGTATAACAAACTTACCACAATGCAGGCCCTTGCAGAAAAAAAAATGCAAGAGTTGGAAGCAAAA CTCCATGAAGAAGAACAGGAAAGGAAACGCATGCAAGCTAAGGCAGCTGAGTTGCAGACTGGTCTAGAA ACAAATAGACTTATCTTTGAAGATAAGGCAACTCCGTGTGTTCCCAATGCAAGAAGAATTAAAAAAAAG AAGTCAAAACCACCAGAAAAGAAAAGTTCTAGGAACTATTTTGGTGCACAACCACATTATAGATTATGC TTGGGTGATATGCCATTTGTAGCTGGGAAGTCCACAAGCCCTAGCCATGCCGTGGTAGCCAATGTTCAG CTTGTCTTGCATCTAATGAAGCAACACAGTAAAGCTTTGTGCAATGATCGAGTCATCAACAGTATTCCT TTGGCAAAGCAAGTATCTTCACGAGGTGGTAAAAGTAAGAAGTTGTCAGTAACACCTCCCTCCTCCAAC GGTATTAATGAGGAGTTGTCAGAAGTCTTACAGACTTTACAGGATGAATTTGGGCAAATGAGCTTTGAT CACCAGCAGCTTGCAAAACTTATCCAGGAGTCGCCAACCGTTGAACTGAAAGACAAGTTGGAGTGTGAA TTGGAGGCATTAGTGGGAAGGATGGAAGCAAAAGCCAACCAAATAACTAAAGTTCGAAAATACCAAGCC CAGCTGGAGAAACAGAAGTTAGAGAAGCAGAAGAAGGAATTAAAAGCTACCAAAAAGACTCTTGATGAA GAAAGAAACAGCAGCAGCCGTTCTGGAATCACAGGGACCACAAATAAGAAAGATTTTATGAAACTGAGA CCTGGAGAAAAAAGGAGAAAAAATCTTCAGTTATTGAAGGACATGCAAAGCATACAGAATTCATTACAA AGCAGTAGTTTGTGTTGGGATTACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001243776
Insert Size	1476 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001243776.1
RefSeq Size	3236 bp
RefSeq ORF	1476 bp
Locus ID	9702
UniProt ID	Q86XR8
MW	56.5 kDa
Gene Summary	This gene encodes a cytoplasmic protein called Translokin. This protein localizes to the centrosome and has a function in microtubular stabilization. The N-terminal half of this protein is required for its centrosome localization and for its multimerization, and the C-terminal half is required for nucleating, bundling and anchoring microtubules to the centrosomes. This protein specifically interacts with fibroblast growth factor 2 (FGF2), sorting nexin 6, Ran-binding protein M and the kinesins KIF3A and KIF3B, and thus mediates the nuclear translocation and mitogenic activity of the FGF2. It also interacts with cyclin D1 and controls nucleocytoplasmic distribution of the cyclin D1 in quiescent cells. This protein is crucial for maintaining correct chromosomal number during cell division. Mutations in this gene cause mosaic variegated aneuploidy syndrome, a rare autosomal recessive disorder. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011] Transcript Variant: This variant (2) has an additional exon in the 5' region, compared to variant 1. The resulting isoform (b) has a shorter and different N-terminus, compared to isoform a.

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