Pescadillo (PES1) (NM_001243225) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NOP7; PES
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331972 representing NM_001243225. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGGAGGCCTTGAGAAGAAGAAGTATGAACGAGGCTCGGCCACCAACTACATCACCCGGAACAAAGCC CGGAAGAAGCTCCAGCTGAGCTTGGCTGACTTTAGGCGGCTGTGCATTCTGAAGGGCATTTATCCCCAT GAACCCAAACACAAGAAGAAGGTTAACAAGGGTTCTACAGCAGCCCGAACGTTTTACCTTATCAAAGAC ATCAGGTTTCTCCTCCACGAACCCATTGTCAACAAGTTCCGTGAATACAAGGTGTTCGTCCGGAAGCTC CGGAAGGCTTATGGGAAGAGCGAGTGGAACACTGTAGAGCGTTTAAAGGACAATAAGCCCAACTACAAA CTCGACCACATCATCAAGGAACGGTATCCCACGTTCATCGATGCCCTGCGGGACCTGGACGATGCCCTC TCCATGTGCTTCCTGTTTTCCACCTTCCCGCGGACTGGCAAGTGCCACGTGCAGACCATTCAGCTGTGC CGCCGGCTCACTGTGGAGTTCATGCACTACATTATCGCTGCCCGTGCCCTGCGCAAGGTCTTCCTGTCC ATCAAAGGCATTTACTACCAGGCCGAGGTACTGGGGCAGCCCATCGTGTGGATCACTCCCTATGCCTTC TCCCATGACCACCCGACAGACGTGGACTACAGGGTCATGGCCACCTTCACCGAGTTCTACACCACGCTG CTGGGCTTTGTCAACTTCCGCCTTTACCAGTTGCTCAACCTCCACTATCCCCCGAAGCTCGAGGGTCAG GCCCAAGCAGAGGCAAAGGCCGGTGAGGGCACCTACGCGTTGGACTCCGAGAGTTGTATGGAGAAACTG GCAGCCCTCAGTGCCAGCCTGGCCCGCGTGGTGGTGCCTGCCACAGAGGAGGAGGCCGAGGTGGATGAG TTTCCCACCGATGGGGAGGAAGACCGCAGGAAGGAGCTGGAGGCGCAGGAGAAGCACAAGAAGCTTTTT GAGGGCCTGAAGTTCTTCCTGAACCGAGAGGTGCCCCGTGAGGCCCTGGCCTTCATCATCAGGAGTTTT GGTGGGGAAGTGTCCTGGGACAAATCTTTGTGCATTGGGGCCACCTATGACGTCACAGACTCCCGCATC ACCCATCAGATTGTCGACCGGCCTGGGCAGCAGACCTCAGTCATTGGCAGGTGCTACGTGCAGCCCCAG TGGGTGTTTGACTCAGTGAACGCCAGGCTCCTTCTCCCCGTGGCAGAGTACTTCTCTGGGGTGCAGCTG CCCCCACACCTTTCACCCTTTGTGACCGAGAAGGAAGGAGATTACGTTCCACCTGAGAAGCTGAAGCTG CTGGCTCTGCAGCGGGGAGAGGACCCAGGAAACCTGAATGAGTCAGAAGAGGAGGAGGAAGAGGACGAC AACAACGAAGGTGATGGTGATGAAGAGGGAGAAAATGAGGAGGAGGAGGAAGATGCAGAGGCTGGTTCA GAAAAGGAGGAAGAGGCCCGGCTGGCAGCCCTGGAAGAGCAGAGGATGGAGGGGAAGAAGCCCAGGGTG ATGGCAGGCACCTTGAAGCTGGAGGATAAGCAGCGGCTGGCCCAGGAGGAGGAGAGTGAGGCCAAGCGC CTGGCCATTATGATGATGAAGAAGCGGGAGAAGTACCTGTACCAGAAGATCATGTTTGGCAAGAGGCGA AAAATCCGAGAGGCCAACAAGCTGGCGGAGAAGCGGAAAGCCCACGATGAGGCGGTGAGGTCTGAGAAG AAGGCCAAGAAGGCAAGGCCGGAGTGA
Restriction Sites	SgfI-RsrII
ACCN	NM_001243225
Insert Size	1752 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001243225.1
RefSeq Size	2297 bp
RefSeq ORF	1752 bp
Locus ID	23481
UniProt ID	O00541
MW	67.5 kDa
Gene Summary	This gene encodes a nuclear protein that contains a breast cancer associated gene 1 (BRCA1) C-terminal interaction domain. The encoded protein interacts with BOP1 and WDR12 to form the PeBoW complex, which plays a critical role in cell proliferation via pre-rRNA processing and 60S ribosomal subunit maturation. Expression of this gene may play an important role in breast cancer proliferation and tumorigenicity. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Pseudogenes of this gene are located on the long arm of chromosome 4 and the short arm of chromosome 9. [provided by RefSeq, Aug 2011] Transcript Variant: This variant (3) uses an alternate splice site in the coding region but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

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