Rad9 (RAD9A) (NM_001243224) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RAD9
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331971 representing NM_001243224. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCAGTCTTTCCTGTCTGTCTTCCGCTCACTGGCGATGCTGGAGAAGACGGTGGAAAAATGCTGCATC TCCCTGAATGGCCGGAGCAGCCGCCTGGTGGTCCAGCTGCATTGCAAGTTCGGGGTGCGGAAGACTCAC AACCTGTCCTTCCAGGACTGTGAGTCCCTGCAGGCCGTCTTCGACCCAGCCTCGTGCCCCCACATGCTC CGCGCCCCAGCACGGGTTCTGGGGGAGGCTGTTCTGCCCTTCTCTCCTGCACTGGCTGAAGTGACGCTG GGCATTGGCCGTGGCCGCAGGGTCATCCTGCGCAGCTACCACGAGGAGGAGGCAGACAGCACTGCCAAA GCCATGGTGACTGAGATGTGCCTTGGAGAGGAGGATTTCCAGCAGCTGCAGGCCCAGGAAGGGGTGGCC ATCACTTTCTGCCTCAAGGAATTCCGGGGGCTCCTGAGCTTTGCAGAGTCAGCAAACTTGAATCTTAGC ATTCATTTTGATGCTCCAGGCAGGCCCGCCATCTTCACCATCAAGGACTCTTTGCTGGACGGCCACTTT GTCTTGGCCACACTCTCAGACACCGACTCGCACTCCCAGGACCTGGGCTCCCCAGAGCGTCACCAGCCA GTGCCTCAGCTCCAGGCTCACAGCACACCCCACCCGGACGACTTTGCCAATGACGACATTGACTCTTAC ATGATCGCCATGGAAACCACTATAGGCAATGAGGGCTCGCGGGTGCTGCCCTCCATTTCCCTTTCACCT GGCCCCCAGCCCCCCAAGAGCCCCGGTCCCCACTCCGAGGAGGAAGATGAGGCTGAGCCCAGTACAGTG CCTGGGACTCCCCCACCCAAGAAGTTCCGCTCACTGTTCTTCGGCTCCATCCTGGCCCCTGTACGCTCC CCCCAGGGCCCCAGCCCTGTGCTGGCGGAAGACAGTGAGGGTGAAGGCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001243224
Insert Size	948 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001243224.1
RefSeq Size	1992 bp
RefSeq ORF	948 bp
Locus ID	5883
UniProt ID	Q99638
Protein Families	Druggable Genome, Stem cell - Pluripotency
MW	34.2 kDa
Gene Summary	This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] Transcript Variant: This variant (2) contains an alternate 5' terminal exon (with an in-frame AUG) compared to variant 1. This results in a a shorter isoform (2) with a distinct N-terminus compared to isoform 1.

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